Thin Layer Chromatography: Analyzing Analgesics Essay Example
Thin Layer Chromatography, also known as TLC, serves as a means of both separating and identifying substances.
Chromatography comes in diverse variations, but a common feature is that there is a fixed and a moving element. In TLC, the silica gel on the tray constitutes the stationary phase, while the mixture under separation makes up the mobile phase. The lab will utilize TLC to assess the polarity of specific mixtures and to enhance comprehension of TLC processes.
To perform TLC, take a TLC plate and use a pencil to mark where you will place the drops. The mark and the spot where the drop is placed should be about 1.0cm from the bottom of the plate. Avoid touching the plate as it can affect the reading. The TLC plates used were thin sheets of plastic co
...ated with silica gel.
Using a capillary tube, a drop each of Ibuprofen, Acetaminophen, Caffeine, and Aspirin were placed either between or on the marks of TLC plates. The same method was used to place a sample of an unknown compound on the third plate. After this, the TLC plates were set aside and a small beaker was used to measure out 7.0 mL of 70% Ethyl Acetate and 3.0 mL of 30% Petroleum Ether, which were covered with a watch glass. The remaining solvent was placed into three jars, ensuring that the level of solvent does not exceed the pencil mark on the TLC plates and is enough to cover the bottom of the jars.
Three jars were used in this experiment. Two of them contained plates with known compounds, while the remaining jar held a plate with an unknown
compound. The plates were positioned at a 45-degree angle inside each jar, and the jars' caps were loosely secured. Throughout the process, it was crucial not to disrupt or move the jars. The solvent was carefully observed as it gradually ascended the TCL plates. Once it reached about a centimeter from the top, the plates were removed from the jars.
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