Which of the following is in the correct order for one cycle of polymerase chain reaction (PCR)?

Denature DNA; anneal primers; extend primers. More test answers on https://studyhippo.com/chapter-20-campbell-biology/

What information is critical to the success of polymerase chain reaction (PCR) itself?

The DNA sequence of the ends of the DNA to be amplified must be known. More test answers on https://studyhippo.com/chapter-20-campbell-biology/

Give two ways in which the polymerase chain reaction differs from the process of transcription. (2)

Transcription uses RNA polymerase; and one template strand whereas PCR uses both strands More test answers on https://studyhippo.com/biology-unit-5-chapter-16-dna-technology/

What does Figure 15-1 represent? a. polymerase chain reaction being used to copy DNA b. gel electrophoresis being used to sequence DNA c. an enzyme being used to cut DNA d. hybridization being used to manipulate DNA

an enzyme being used to cut DNA More test answers on https://studyhippo.com/living-environment-chapter-15/

Which of the following enzymes is key to the automation of PCR (polymerase chain reactions)?

taq polymerase More test answers on https://studyhippo.com/bio-203-ch-20-practice-test-questions/

(Refer to exam q) The polymerase chain reaction is a process which can be carried out in a laboratory to replicate DNA. The diagram shows the main stages involved in the polymerase chain reaction. Explain why DNA is heated to 95 °C. (1)

To separate the two strands More test answers on https://studyhippo.com/biology-unit-5-chapter-16-dna-technology/

Which of the following is mismatched? A. bioinformatics – the study of a genomic and proteomic information using computer analysis B. polymerase chain reaction – process that separates DNA fragments according to size C. genomics – the study of the genomes of humans and other organisms D. proteomics – the study of species’ proteins

polymerase chain reaction – process that separates DNA fragments according to size More test answers on https://studyhippo.com/biotechnology-and-genomics/

1. DNA/PCR polymerase chain reaction-based technique 2. high-complexity stool culture 3.proteomic-based mass spectronomy

The 3 methods of stool testing are: More test answers on https://studyhippo.com/kresser-institute-week-6-gut-stool-testing/

To carry out a polymerase chain reaction (PCR), you must have DNA polymerase and

the nucleotides to synthesize new DNA strands. More test answers on https://studyhippo.com/biotechnology-and-genomics/

The function of the polymerase chain reaction in genetic engineering is to

make multiple copies of a specific segment of DNA. More test answers on https://studyhippo.com/biotechnology-and-genomics/

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Polymerase Chain Reaction Flashcards, test questions and answers

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What is Polymerase Chain Reaction?

The Polymerase Chain Reaction (PCR) is a revolutionary technique used in molecular biology that allows researchers to rapidly and efficiently amplify specific regions of DNA. PCR has revolutionized the study of genetics and other fields by allowing scientists to quickly replicate and study small segments of DNA. This process can be used for a variety of purposes, including medical diagnostics, gene cloning, genetic engineering, forensics, evolutionary studies, and studying ancient organisms.At its core, PCR is a three-step process in which two primers are used to identify the target gene sequence in a sample of DNA. The primers bind to complementary areas on either side of the targeted sequence and act as a starting point for replication. Next, an enzyme called Taq polymerase is added which begins synthesizing new copies of the target sequence from the two primers as templates. Finally, heat is applied which causes the newly created strands of DNA to separate from each other so that they can be replicated again with more Taq polymerase added back into the mix each cycle. After several cycles have been completed many millions or billions copies of this particular segment are generated which can then be utilized for further analysis or manipulation. PCR has been instrumental in advancing our knowledge in many areas such as identifying disease causing-genes at early stages or even prenatal diagnosis; forensic identification; creating diagnostic tests based on gene expression; and improving crop yields through genetic engineering techniques such as marker-assisted selection (MAS). In addition it also allows us to quickly detect pathogens such as viruses or bacteria without having to wait days for their growth cultures in traditional laboratories which would take far too long especially when dealing with serious illnesses like HIV/AIDS where time may be critical for life saving treatments. Overall PCR has dramatically impacted both basic research and clinical practice by providing efficient means for amplifying small amounts of DNA into large quantities that can then be analyzed quickly thereby allowing us to gain insight into complex biological processes with greater accuracy than ever before possible.