Protozoa – Microbiology Test Questions – Flashcards
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| Pneumocystis carinii ID methods |
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| PCP test on sputum (formalin) sample, histology stain |
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| Pneumocystis carinii Diagnosis |
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| fungi characteristics |
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| Pneumocystis carinii special characteristics |
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| causes fulminant lung infections in AIDS patients, fungi, Sporozoa subclass Coccidia |
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| Isospora belli ID methods |
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| MAF + with stool sample |
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| Isospora belli diagnosis |
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| 2 sporocysts in oocysts, each sporocyst contains four sporozoites |
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| Isospora belli special characteristics |
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| : intestinal parasite, nonpathogenic (usually), Sporozoa subclass Coccidia |
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| Microsporidia (Encephalitozoon sp., Enterocytozoon sp., and Microsporidium sp.) ID methods |
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| PCR to ID, trichrome stain on stool |
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| Microsporidia (Encephalitozoon sp., Enterocytozoon sp., and Microsporidium sp.) diagnosis |
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| 1-2 uM, tiny |
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| Microsporidia (Encephalitozoon sp., Enterocytozoon sp., and Microsporidium sp.) special characteristics |
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| can infect tissue, Sporozoa subclass Coccidia |
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| Cryptosporidium parvum ID Methods |
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| MAF+, EIA (with Giardia) |
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| Cryptosporidium parvum diagnosis |
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| 4 sporozoites free |
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| Cryptosporidium parvum special characteristics |
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| AIDs, Sporozoa subclass Coccidia |
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| Giardia lamblia ID Methods |
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| trichrome stain, Rapid EIA (with crypto) |
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| Giardia lamblia diagnosis |
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| cyst: 4 nuclei without flagella, dominant and protective stage troph: looks like eyes with 4 flagella, 2 nuclei, and trophs are larger they are reproducing and feeding (active), nucleus contains karisome |
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| Giardia lamblia special characteristics |
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| fatty diarrhea, suction disk that adheres to intestine and blocks absorption, transmitted via F/O route by contaminated water, 1 of the most common intestinal parasite, flagella |
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| Trichomonas vaginalis ID methods |
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| urethra swab, urine, vaginal swab for wet prep |
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| Trichomonas vaginalis diagnosis |
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| no cyst stage, nucleus undulating membrane, flagella and motile |
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| Trichomonas vaginalis special characteristics |
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| can be seen in urine, STD, flagella |
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| Balantidium Coli ID methods |
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| concentrated wet prep with iodine, not seen on trichrome |
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| Balantidium Coli diagnosis |
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| cyst, cilia and macronucleus on troph |
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| Balantidium Coli special characteristics |
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| normal flora can be pathogenic and cause dysentery, cyst from contaminated water, ciliate |
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| Entamoeba histolytica ID methods |
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| trichrome smear from stool, PVA tube |
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| Entamoeba histolytica diagnosis |
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| trophozoite with ingested rbcs, pseudopods for motility, 15-20 uM, beaded chromatin, 1-4 nuclei in cyst, vacuole, and chromatid bodies |
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| Entamoeba histolytica special characteristics |
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| pathogenic can cause bloody diarrhea, intestinal amoeba |
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| Entamoeba hartmanii ID methods |
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| trichrome stain |
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| Entamoeba hartmanii diagnosis |
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| no rbcs, smaller 8-10 uM, 1 nucleus, solid dense chromatin |
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| Entamoeba hartmanii special characteristics |
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| nonpathogenic, intestinal amoeba |
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| Entamoeba coli ID methods |
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| trichrome |
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| Entamoeba coli diagnosis |
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| bigger 20-25 uM, 1 nucleus in troph, 1-8 nuclei in cyst, karisome is off to the side, karisome is inside nucleus, condensed chromatin |
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| Entamoeba coli special characteristics |
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| nonpathogenic, intestinal amoeba |
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| Endolimax nana ID methods |
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| trichrome |
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| Endolimax nana diagnosis |
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| cysts are larger than trophs; trophs are 6-12 uM and has 1 nucleus, 1-4 nuclei in cysts, karisome clumped and irregular, no chromatin, fine cytoplasm |
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| Endolimax nana special characteristics |
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| intestinal amoeba |
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| Iodamoeba butschlii ID methods |
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| Trichrome, direct with iodine |
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| Iodamoeba butschlii diagnosis |
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| : large glycogen vacuole (stains with iodine), 8-10uM, vacuole pushed nucleus to the side, 1 nucleus in both cyst and troph, no chromatin |
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| Iodamoeba butschlii special characteristics |
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| vacuole stains with iodine, intestinal amoeba |
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| Blastocystis hominis ID methods |
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| trichrome |
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| Blastocystis hominis diagnosis |
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| large vacuole is the central body pushed nucleus to periphery, 6-40uM variation in size, 4 nuclei in cyst |
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| Blastocystis hominis special characteristics |
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| vacuole is 90% of organism, variation in size, intestinal amoeba |
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| Acanthamoeba spp ID methods |
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| : grow in culture (?), eye scraping, wet prep with LM |
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| Acanthamoeba spp diagnosis |
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| brain abscess |
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| Acanthamoeba spp special characteristics |
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| infect contacts cleaned in water (not solution), keratitis, tissue amoeba |
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| Acanthomba in brain tissue |
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| Acanthoamoba in corneal scrapping |
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| Balantidium Coli |
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| B. coli troph |
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| Blastocystis hominis |
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| Crytospordium paruvum |
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| E. nana |
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| E. hartmarni |
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| E. histolytica cyst |
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| E. histolytica troph |
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| G. lamblia cyst |
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| G. Lamblia trichrome |
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| G. lamblia troph |
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| Iodamoeba butshlii iodine |
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| iodamoeba butshlii trichrome |
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| isopora belli |
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| Microsporidia |
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| Naegleria fowleri |
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| Pneumocystitis carinii |
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| Trichomonas vaginalis troph |
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| Trichomonas vaginalis wet mount |
