proteins E

question

What is the process of destroying the native conformation of a protein by chemical or physical means?
answer

Denaturation
question

What is denaturation and is it reversible
answer

Pocess of destroying the native conformation of a protein by chemical or physical means and can or cannot be reversible
question

Name an irreversible denaturing agent
answer

Heat-disrupts hydrogen bonding and precipitation may take place
question

Name two ways to denature a protein tha is reversible?
answer

6M aqueous urea; alcohols-70% ethanol disrupts hydrogen bonding
question

What ions form water-insoluble salts with SH groups when denaturing a protein?
answer

Heavy metal ions Pb2+, Hg2+, and Cd2+
question

Which reducing agents form reversible denaturizing reactions with proteins?
answer

2-mercaptoethanol cleaes disulfide bonds by reducing s-s groups to s-H groups
question

Do detergents denature proteins reversibly?
answer

Yes, surfact-active agents sodium dedecylulfate, sds distupt hydrogen bonding

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