proteins E

Flashcard maker : Henry Lowe
What is the process of destroying the native conformation of a protein by chemical or physical means?
Denaturation
What is denaturation and is it reversible
Pocess of destroying the native conformation of a protein by chemical or physical means and can or cannot be reversible
Name an irreversible denaturing agent
Heat-disrupts hydrogen bonding and precipitation may take place
Name two ways to denature a protein tha is reversible?
6M aqueous urea; alcohols-70% ethanol disrupts hydrogen bonding
What ions form water-insoluble salts with SH groups when denaturing a protein?
Heavy metal ions Pb2+, Hg2+, and Cd2+
Which reducing agents form reversible denaturizing reactions with proteins?
2-mercaptoethanol cleaes disulfide bonds by reducing s-s groups to s-H groups
Do detergents denature proteins reversibly?
Yes, surfact-active agents sodium dedecylulfate, sds distupt hydrogen bonding

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