proteins E – Flashcards
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| What is the process of destroying the native conformation of a protein by chemical or physical means? |
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| Denaturation |
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| What is denaturation and is it reversible |
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| Pocess of destroying the native conformation of a protein by chemical or physical means and can or cannot be reversible |
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| Name an irreversible denaturing agent |
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| Heat-disrupts hydrogen bonding and precipitation may take place |
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| Name two ways to denature a protein tha is reversible? |
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| 6M aqueous urea; alcohols-70% ethanol disrupts hydrogen bonding |
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| What ions form water-insoluble salts with SH groups when denaturing a protein? |
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| Heavy metal ions Pb2+, Hg2+, and Cd2+ |
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| Which reducing agents form reversible denaturizing reactions with proteins? |
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| 2-mercaptoethanol cleaes disulfide bonds by reducing s-s groups to s-H groups |
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| Do detergents denature proteins reversibly? |
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| Yes, surfact-active agents sodium dedecylulfate, sds distupt hydrogen bonding |
