Exam 3 Biochem-Lecture 28 – Flashcards

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Desaturation of Fatty Acids requires combination of

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  • desaturase enzyme
  • cytochrome b5
  • NADPH-cytochrome b5 reductase
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Triacylglycerols are synthesized from:
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  • Fatty Acyl CoAs
  • Glycerol 3-Phosphate
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The first step  of Synthesis of Triacylglycerols is formation of  ......?
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phosphatidic  acid.

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Fatty acyl CoAs are formed ....?
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outside mitochondria
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What is the transport molecule for fatty acids into the mitochondrial matrix???
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Carnitine
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FAS are oxidized sequentially how many carbons at a time???
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FAS are oxidized sequentially 2 carbons at a time.
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Sequential steps of  Fatty Acid b-Oxidation are:

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  1. Dehydrogenation
  2. Hydration
  3. Oxidation
  4. Cleavage
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Fatty Acid b-Oxidation is Oxidized while attached as:

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as a thioester to 4-phosphopantetheine of CoA
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Overall yield of  Fatty Acid b-Oxidation is

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106 ATPS.

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b-oxidation oxidizes saturated fatty acids with even or odd number???
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b-oxidation oxidizes saturated fatty acids with even number
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In Fatty Acid Oxidation, Odd-chains produces
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propinolyl CoA
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•In Fatty acid Oxidation, Unsaturated fatty acids require ....?
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additional enzymes
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In Fatty Acid Oxidation, a- oxidation is necessary for
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  • for metabolism  of branched chain fatty acid
  • uses fatty acid a hydroxylase
  • occurs in peroxisomes
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Ketone Bodies
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  • an alternate fuel for cells
  • Water soluble lipid based energy
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Ketone Bodies formation occurs
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 in the mitochondrial matrix
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 b-hydroxy-b-methylglutaryl CoA (HMG-CoA) is intermediate in
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 b-hydroxy-b-methylglutaryl CoA (HMG-CoA) is intermediate in acetoacetate synthesis from acetyl-CoA.
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In Ketone Body synthesis, 

2 acetyl-CoA molecules are condensed

to form ...............by b-ketothiolase.

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In Ketone Body synthesis,   

2 acetyl-CoA molecules are condensed

to form acetoacetyl-CoA by b-ketothiolase.

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In Ketone Bodies synthesis, Acetoacetyl-CoA is condensed with a second acetyl-CoA to form HMG-CoA by what????

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In Ketone Bodies synthesis, Acetoacetyl-CoA is condensed with a second acetyl-CoA to form HMG-CoA by HMG-CoA synthase.

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In Ketone Bodies synthesis, what is condensed with a second acetyl-CoA to form HMG-CoA by HMG-CoA synthase???

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In Ketone Bodies synthesis, Acetoacetyl-CoA is condensed with a second acetyl-CoA to form HMG-CoA by HMG-CoA synthase.

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In Ketone Bodies synthesis, Acetoacetyl-CoA is condensed with what to form HMG-CoA by HMG-CoA synthase.???

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In Ketone Bodies synthesis, Acetoacetyl-CoA is condensed with a second acetyl-CoA to form HMG-CoA by HMG-CoA synthase.

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In Ketone Bodies synthesis, Acetoacetyl-CoA is condensed with a second acetyl-CoA to form what by HMG-CoA synthase???

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In Ketone Bodies synthesis, Acetoacetyl-CoA is condensed with a second acetyl-CoA to form HMG-CoA by HMG-CoA synthase.

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HMG-CoA is cleaved by HMG-CoA lyase

to yield

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  • acetoacetic acid 
  •  acetyl-CoA.
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Acetoacetic acid is reduced to d-b-hydroxy-

butyrate by b-hyrdoxybutyrate dehydrogenase to yield hydroxybutyrate at the expense of how many NADH??.

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Acetoacetic acid is reduced to d-b-hydroxy-

butyrate by b-hyrdoxybutyrate dehydrogenase to yield hydroxybutyrate at the expense of 1 NADH.

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What is reduced to d-b-hydroxy-

butyrate by b-hyrdoxybutyrate dehydrogenase to yield hydroxybutyrate at the expense of 1 NADH???

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Acetoacetic acid is reduced to d-b-hydroxy-

butyrate by b-hyrdoxybutyrate dehydrogenase to yield hydroxybutyrate at the expense of 1 NADH.

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Acetoacetic acid is reduced to what by b-hyrdoxybutyrate dehydrogenase to yield hydroxybutyrate at the expense of 1 NADH???
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Acetoacetic acid is reduced to d-b-hydroxy-

butyrate by b-hyrdoxybutyrate dehydrogenase to yield hydroxybutyrate at the expense of 1 NADH.

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Acetoacetic acid spontaneously forms
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  • acetone
  • + CO2.
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Absence of HMG-CoA lyase results in HMG-CoA being used for ???
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cholesterol synthesis
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Ketone Bodies can be used by
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Brain

Muscle

Other tissues

BUT NOT LIVER(Liver can not use ketone bodies)

 

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