Chemistry Internship Study Questions Section 2 – Flashcards

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question
Why should GRAY top and PURPLE top tubes be AVOIDED when doing electrolyte testing?
answer
They interfere with Calcium and also Contain Potassium
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Which electrolytes are most affected by hemolysis?
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Potassium & Phosphate
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Fill in the chart. For "highest" and "lowest" levels, list only the most common conditions:
A
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Extra
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Fill in the chart. For "highest" and "lowest" levels, list only the most common conditions:
B
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Intra
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Fill in the chart. For "highest" and "lowest" levels, list only the most common conditions:
C
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Extra
question
Fill in the chart. For "highest" and "lowest" levels, list only the most common conditions:
D
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Cation
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Fill in the chart. For "highest" and "lowest" levels, list only the most common conditions:
E
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Cation
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Fill in the chart. For "highest" and "lowest" levels, list only the most common conditions:
F
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Anion
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Fill in the chart. For "highest" and "lowest" levels, list only the most common conditions:
G
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Dehydration, Cushing's, Diabetes insipidus
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Fill in the chart. For "highest" and "lowest" levels, list only the most common conditions:
H
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Hemolysis, Tissue trauma, Renal failure, Burns, Diabetes
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Fill in the chart. For "highest" and "lowest" levels, list only the most common conditions:
I
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Dehydration, Metabolic acidosis
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Fill in the chart. For "highest" and "lowest" levels, list only the most common conditions:
J
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Addison's Disease, Diuretics, Diarrhea, Kidney Disease, Heart Failure
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Fill in the chart. For "highest" and "lowest" levels, list only the most common conditions:
K
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Alkalosis, Insulin Excess
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Fill in the chart. For "highest" and "lowest" levels, list only the most common conditions:
L
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Vomiting, Renal Failure
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Fill in the chart. For "highest" and "lowest" levels, list only the most common conditions:
M
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135-145 mEq/L
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Fill in the chart. For "highest" and "lowest" levels, list only the most common conditions:
N
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3.5-5.0 mEq/L
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Fill in the chart. For "highest" and "lowest" levels, list only the most common conditions:
O
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100-109
(Mayo Clinic >/= 18 years:98-107, <18 years: 102-112)
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Fill in the chart. For "highest" and "lowest" levels, list only the most common conditions:
P
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<120
>155
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Fill in the chart. For "highest" and "lowest" levels, list only the most common conditions:
Q
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<3
>6
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Fill in the chart. For "highest" and "lowest" levels, list only the most common conditions:
R (UAMS neonates)
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<90 mmol/L
>120 mmol/L
question
Define osmolality
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Number of particles present per unit of solution;
Proportional to concentration
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What are accepted normal ranges for osmolality for plasma?
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280-300 m Osm/kg
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What are accepted normal ranges for osmolality for urine?
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300-900 m Osm/kg
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How will an increased osmolality affect each of the following colligative properties:

Freezing Point
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Decreased value
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How will an increased osmolality affect each of the following colligative properties:

Vapor Pressure
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Decreased value
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How will an increased osmolality affect each of the following colligative properties:

Boiling Point
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Increased value
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How will an increased osmolality affect each of the following colligative properties:

Osmotic Pressure
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Increased value
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What is the formula from class that is used to calculate osmolality?
answer
2(Na) + Glucose/20 + BUN/3
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Calculate an osmolality from the following data (use the formula from class):
BUN 20 mg/dL
Glucose 100 mg/dL
Sodium 140 mEq/L
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292 mOsm/kg
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What are the most common conditions responsible for hyperosmolality of serum?
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Diabetes mellitus, Dehydration, Renal Failure, and Hyperproteinemia
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What is the formula for delta osmolality?
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Measured-Calculated
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What is the normal range for delta osmolality?
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0-10
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What 3 major substances are most commonly associated with an elevated delta osmolality?
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Methanol, isopropanol, ethylene glycol. Increased lipids, protein, or glucose
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What is the most common cause of a delta osmolality <0?
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Error
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What is meant by "anion gap"?
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Estimate of unmeasured electrolyte
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Why is an "anion gap" measured?
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Used to assess acid-base disorders and lab QC
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Give the formula for the anion gap
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(Na + K) - (Cl + HCO3)
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What is the normal range for the "anion gap"?
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12-20 mmol/L
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How will the "anion gap" be affected in the following conditions? (increased, decreased, or unaffected)
Salicylate Poisoning
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Increased
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How will the "anion gap" be affected in the following conditions? (increased, decreased, or unaffected)
Ketosis
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Increased
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How will the "anion gap" be affected in the following conditions? (increased, decreased, or unaffected)
Dehydration
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Increased
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How will the "anion gap" be affected in the following conditions? (increased, decreased, or unaffected)
Ethylene Glycol Poisoning
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Increased
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What is the most likely cause of an AG<5?
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Lab Error OR Significant Increase in Na, K, Cl, or HCO3
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What is the difference between a direct and indirect ISE analysis?
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Direct - NO Sample Dilution required
Indirect - Requires Sample Dilution
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What are the main sources of interference in the indirect ISE test?
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Increased Lipids & Proteins
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What is the clinical significance of an elevated sweat chloride test?
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Cystic Fibrosis
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What is the name of the procedure/technique used to collect sweat for analysis?
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Pilocarpine iontophoresis
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Which 2 analytes, when markedly decreased in serum, can cause tetany?
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Calcium & Magnesium
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Why are oxalate & EDTA unacceptable anticoagulants for calcium analysis?
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Both chelate Calcium, causing falsely Decreased results
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Why is the determination of ionized Calcium important?
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This form is NOT bound to protein and is a better reflection of Calcium levels in patients that may have protein abnormalities or liver disease
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What effect does each of the following have on serum Calcium levels:
PTH
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Increased
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What effect does each of the following have on serum Calcium levels:
Calcitonin
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Decreased
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What effect does each of the following have on serum Calcium levels:
Vitamin D
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Increased
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What is the most common cause of elevated plasma Calcium?
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Hyperparathyroidism
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What common plasma cell disease is also associated with elevated Calcium?
answer
Multiple Myeloma (MM)
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How does the Albumin concentration influence the level of Calcium?
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Most Calcium is Albumin bound so when there are Increased levels of Albumin there will likely be Increased levels of Calcium
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Where is most body Iron found?
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Blood / RBCs (Hemoglobin)
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What is the carrier protein for Iron?
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Transferrin
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What is the storage form of Iron?
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Ferritin
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Where is the storage form of Iron stored?
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Liver & Bone Marrow
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What are the most common causes of Iron Deficiency Anemia (IDA)?
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Chronic Blood Loss, Pregnancy, Childhood, & Dietary Insufficiency
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Describe the typical RBC morphology in Iron Deficiency Anemia (IDA)
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Microcytic, Hypochromic
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What is the purpose of the TIBC test?
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Indirect measure of Transferrin. Used to differentiate causes of Iron deficiency.
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How is TIBC calculated / What is the formula for TIBC?
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TIBC = Transferrin * 1.4
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What is %Tf Saturation?
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Measures the degree to which the binding sites are saturated with Iron
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What is the formula for %Tf Saturation?
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(Fe/TIBC)x100
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Under what conditions (test results) can the TIBC & %Tf Saturation NOT be calculated?
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Fe <5 OR Transferrin <70
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Complete the chart below. Indicate whether the following conditions are typically associated with Increased, Normal, or Decreased test results:
A
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Decreased
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Complete the chart below. Indicate whether the following conditions are typically associated with Increased, Normal, or Decreased test results:
B
[image]
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Increased
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Complete the chart below. Indicate whether the following conditions are typically associated with Increased, Normal, or Decreased test results:
C
[image]
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Decreased
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Complete the chart below. Indicate whether the following conditions are typically associated with Increased, Normal, or Decreased test results:
D
[image]
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Decreased
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Complete the chart below. Indicate whether the following conditions are typically associated with Increased, Normal, or Decreased test results:
E
[image]
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Decreased
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Complete the chart below. Indicate whether the following conditions are typically associated with Increased, Normal, or Decreased test results:
F
[image]
answer
Decreased
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Complete the chart below. Indicate whether the following conditions are typically associated with Increased, Normal, or Decreased test results:
G
[image]
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Decreased
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Complete the chart below. Indicate whether the following conditions are typically associated with Increased, Normal, or Decreased test results:
H
[image]
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Normal / Increased
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Complete the chart below. Indicate whether the following conditions are typically associated with Increased, Normal, or Decreased test results:
I
[image]
answer
Increased
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Complete the chart below. Indicate whether the following conditions are typically associated with Increased, Normal, or Decreased test results:
J
[image]
answer
Normal / Decreased
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Complete the chart below. Indicate whether the following conditions are typically associated with Increased, Normal, or Decreased test results:
K
[image]
answer
Increased
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Complete the chart below. Indicate whether the following conditions are typically associated with Increased, Normal, or Decreased test results:
L
[image]
answer
Increased
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