Ashitaba as an Alternative to Commercial Hand Sanitizer Essay Example

the arm were selected. A swab head moistened with sterile water was rubbed three times in a rotating motion on each surface. The swab heads were then placed into tubes and shaken for a total of 50 cycles lasting 10 seconds each. A rinse solution diluted at a ratio of 1:10 was prepared and poured onto soy agar plates in portions of both 1.0 ml and 0.1 ml (triplicate). After incubating for 1-2 days at ICC temperature, the number of colonies per ml in the rinse solution was calculated using a specific formula to determine the microbial load on the skin surface.

The second marked area on the skin was wetted and rubbed with the hand sanitizer for 15 seconds. Meanwhile, for comparison, soapless lathering for 60 seconds followed by rinsing with sterile water for another 15 seconds took place on the first area. Some steps were repeated to establish COIF/CM values for this second washed region.

Additionally, bacterial cultures were treated with extracts from Assistant leaves to assess their antibacterial activity after seven days. The plates were examined based on zone diameter inhibition measurements. Finally, hand sanitizer containing these extracts was created and tested specifically on a designated part of the skin.
I compared the amount and composition of microorganisms on treated and untreated skin surfaces as part of my science investigatory project. I will upload the remaining chapters once I have completed my study.