Enzyme Lab Essay Example

caused exothermic. In the body hydrogen peroxide or H2O2 is separated and oxygen and water are released into the body. Thus, the reaction gives the body hydrogen peroxide or H2O2 and oxygen, two essential needs for the human body, from something that could possibly poison or kill your body. Although there were excellent reactions, there were several conditions that slowed down and stopped the action of the enzyme catalase. One condition was when the liver was boiled.

The liver hardened and it did not react to the hydrogen peroxide or H2O2.Also, when reused hydrogen peroxide or H2O2 was used, there was not a reaction. Catalase has an optimal temperature and pH for that of a human cell. The optimal temperature is average room temperature, which is about 25°C.

With the class averages, it had the highest rate of reaction with a 3. 38. Also, the optimal pH was a neutral one of 7. By comparing this to the class data, ph 7 had a rate of reaction over 4, giving it the highest rate of reaction of all the pH levels tested. These pH and temperature levels are good because in the human body, there is a low pH and a constant body temperature.When the temperature is changed, either lower or higher, the enzyme activity is greatly affected.

When the liver was boiled, there was a little to no reaction. Also, with different pH levels, catalase acts very differently. The difference between the acidic pH levels and base-like pH levels were very different. In the lab, we were able to determine which tissues had catalase present. All of the tissues appeared to have catalase present in them.

Although is

seemed that all the tissues contained catalase, they did not have the same amount or quantity of it.The tissue with the lowest rate of reaction, a rate of . 52, was the piece of apple. The tissue with the highest average rate of reaction was liver. Liver had an average rate of reaction of 3. Because the body creates so many deadly poisons, catalase needs to be present in order to speed up the rate of reaction.

The liver and the hydrogen peroxide or H2O2 reacted through hydrolysis. The hydrogen peroxide or H2O2 was separated by taking out the water that bonded it. The gas that was produced was O2 or oxygen. One can tell that O2 or oxygen was produced because it the only gas the supported burning.When the class did the demo, the splint stayed lit in the test tube, proving that oxygen was present. The liquid that was left behind was H2O better known as water.

When hydrolysis took out the oxygen in the bond, the only thing that was left was H2O2. The group data compared to the class averages weren’t that different. To start with, the used hydrogen peroxide or H2O2 with the new liver were off. The rate of reaction that the group gave was a 1 while the class average was .

This might have happened by not pouring all of the used hydrogen peroxide or H2O2 into the new test tube with the new liver.Also, the warm liver and hydrogen peroxide or H2O2 varied by a 2. 33 difference. This may have occurred by not having a constant temperature of 25°C. Another big difference that we had was with the

hydrogen peroxide/ H2O2, liver, and acid pH3.

Our group data showed that we had given the rate of reaction a 3 while the group data’s average was a 1. 63. I think that when we performed this part of the lab, we may have not fully used 2 mL of H2O2 because we getting close to the end of the lab and we decided to use as much of it as we could of without having to open another bottle.