Chapter 4 – Microbiology Answers – Flashcards

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Bacteria used as insecticide
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Bacillus thuringensis
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Bacteria used in warfare causing 1. anthrax and 2. food poisening
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Bacillus anthracis and Clostridium botulinum
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5 features of prokaryotes
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1. one circular chromosome
2. no histones
3. no organelles
4. peptidoglycan cell walls
5. binary fission
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Bacillus
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rod shaped
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coccus
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spherical
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spirillum
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wave shaped
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vibrio
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curved rod
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spirochete
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corkscrew
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strepto
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chain
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staphyl
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cluster
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8 cell
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sarcinae
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2 examples of pleomorphic bacteria
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1. Rhizobium
2. Corynebacterium
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adavtages of having small size (3)
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1. accumulate nutrients quickly
2. metabolize quickly
3. grow quickly
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largest bacteria known
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Epulopiscium sp.
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What is glycocalyx made of?
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polysaccarhides
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2 functions of glycocalyx
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1. source of food under adverse conditions
2. absorbs water, prevents dehydration
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biofilm is associated with this type of glycocalyx
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slime layer
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why does a capsule increase pathogencity of bactuera?
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white blood cells cannot grab them, prevents phagocytosis
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3 functions of cell wall
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rigidity, shape, prevents osmotic lysis
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Tetrapeptide side chaings hang from...
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NAM
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Peptide cross bridges do what
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link layers of PG together
maintain integrety of cell wall
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# layers of PG in Gram + cell wall
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10-12
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Teichoic acids roles and composition
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present in gram +
regulate movement of cations

alcohol/glycerol and phosphate
provide antigenic variation
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antigentic variation
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ability of distinguish between bacterial cells
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Gram negative cell wall
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thin layer of PG
no teichoic acids
outer membrane
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composition of outer membrane in gram neg
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LPS (lipopolysarcharide)
-made of Lipid A and O polysaccharide
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Lipid A
O polysaccharide
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endotoxin
antigen, used for distinguishing bacteria
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example of a bacteria whose antigen identifies it
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E Coli O157:H7
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how penecillan works
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kills PG in gram + bacteria, specifically by breaking peptide cross bridges
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acid fast cell wall composition
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waxy lipid (mycolic acid) bound to peptidoglycan
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2 examples of acid fast cell wall bacteria
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1. Mycobacterium - causes TB
2. Nocardia
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mycoplasma bacteria
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-lack cell walls
-there are sterols in plasma membrane help protect from lysis
-smallest of all bacteria, most contaminating in labs
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archea cell wall composition
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pseudomurein or pseudoPG
lacks NAM and D-amino acids
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Plasma membrane composition
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phopholipid bilayer and proteins
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fluid mosaic model
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lipids and proteins are free to move around, about as viscous as olive oil
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functions of plasma membrane
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-selective permiability
-enzymes for ATP production (lack mitochondria)
-photosynthetic pigments
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damage to plasma membrane caused by
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1. alcohol
2. detergent
3. polymyxin antibiotics
casue leakage,death
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simple diffusion
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passive transport, usually gaseous molecules
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facilitated diffusion
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passive, larger molecules ex. glycerol
goes through transporter protein
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osmolerity in cell
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0.85%, ex. saline
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classical active transport
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-uses ATP or PMF
-substance not altered across membrane
-uses transporter proteins
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group translocation
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molecular is chemically altered, this prevents it from leaving cell
ex. glucose is phosphorolated
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what bacteria does not have flagella?
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cocci
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flagellin proteins in the filament are
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H proteins, recognized by immune system
ex. E Coli
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monotrichous
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1 flagellum at 1 end
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ampthitrichous
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1 flagellum at each end
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lophotrichous
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many flagellum at one end
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peritrichous
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entire cell wall has flagella
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chemotaxis and phototaxis
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movement towards or away from a chemical or light
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what bacteria causes stomach ulcers
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Helicobacter pylaorii
strong flagella can swim through mucous
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what bacteria causes stomach ulcers?
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Helicobacter pylaorii
strong flagella can swim through mucous
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mode of movement by bacteria in ocean
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gaseous vesicles
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what type of cells have pili and fimbriae?
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gram negative
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function of pili
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gliding/twitching movement
DNA transfer
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Fimbriae
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used to attach to things, eg inside of small intestine
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structures found in cytoplasm
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DNA and ribosomes
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bacterial chromosome (nucleoid) characteristics (4)
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1. no nuclear membrane
2. no histones
3. circular DNA
4. Haploid DNA (only 1 copy of each gene)
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plasmids description
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circular, double stranded DNA, extra-chromosomal
autonomis from nucleoid
not required by cell
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benefits of plasmids
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can contain genes for:
-antibiotic resistance
-toxic metal resistance
-production of enzymes
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transfer of plasmids
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takes place through pili
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function of ribosomes
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make proteins
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somposition of bacterial ribosome
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small subunit = 30S
large subunit = 50S
complete ribosome = 70S
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human ribosome complete unit
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80S
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antibiotic effect on ribosomes
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antibiotics can be targeted towards bacterial ribosomes without harming human ribosomes
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content and use of metachromatic granule
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phosphate, used to make ATP
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presence of metachromatic granule indicates what species of bacterium?
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Corynebacterium diphtheriae
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who produces endospores?
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gram +
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when are endospores produced?
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when a bacterium is stressed because of lack of C or N
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how long can endospores survive?
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100k+ years
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the process of endospore formation is known as
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sporulation
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endospores are made of what?
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DPA dipicolnic acid
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