NHA CPT studyguide

Order of Draw: Blood Culture
1st, YELLOW , Sodium polyethenal sulfonate, 8 inversions, Microbiology

Order of Draw: Coagulation
2nd, LIGHT BLUE, Sodium citrate , 3-4 inversions, Chemistry, Common tests: PT, PTT, APTT, and INR

3rd, RED, no additives, no inversions, Chemistry, common tests: CMP, BMP, Hepatitis panel, therapeutic drug level

4th, GREEN, heparin, 8 inversions, STAT Chemistry, Common tests: Amonia, plasma, stat whole blood, BMP-Basic Metabolic Panel, CMP-Comprehensive metabolic panel, troponin

Whole Blood (hematology cell counting)
5th, LAVENDER, EDTA, 8 inversions, Hematology, Common Tests: CBC, lead, ESR, Retic

6th, GREY, Sodium fluoride/ Potassium oxalate, 8 inversions, Chemistry, Common tests: glucose, alcohol, latic acid

The THREE phases of Hemostasis
1. Vasoconstriction 2. Platelet formation 3. Clotting

Blood Thinners
Warfarin (coumadin) , Asparin

Pulmonary System
Blood is oxygenated and returned to the heart to be sent to systemic system. (note: pulmonary arteries)

Oxygenated blood from Left side of the heart to body muscular walls, and to the nerve supply

Systemic system
Blood through whole body to deliver and remove oxygen and waste

membrane- bound packets of cytoplasm (*note: no dna, or nucleus)

Thixotropic Gel
Inert, synthetic substance makes a barrier between plasma and formed elements

Tiny blood vessels (smallest) thin pourus walls, carries oxygen to tissues

Thin walls, Deoxygenated blood to the right side of the heart, lack of nerve supply

Blood Pressure
Normal BP= 120/80 mm/hg, High BP= greater than or equal to 140/90 mm/Hg, Low BP= < 120/80 mm/Hg

Tube Sizes
Adult: 3-10mL, Pediatric: 2-4mL

Blood Smears
Feathered Edge

Number of Leukocytes (white blood cells)
5 Types

Formed Elements
Cellular elements such as RBC. 45% of a blood sample is formed elements

Inactivates thrombin and thromboplastin

Whole Blood
Blood collected with an anticoagulant used for hematology tests

Binds Calcium

Order of Cappilary Draw

Excess of iron in the blood

Destruction of Red Blood Cells (RBCs)

Process by which the body stops blood flow from a leaking wound

Chain of Infection
1. Causitive agent 2. Resevoir 3. Portal of opening 4. Mode of transmission 5. Portal of entry 6. Succeptable host

Sodium fluoride and Potassium oxalate
Binds Calcium

Baby Lancet Size/ Depth
0.65mm per 1kg

Fluid portion of blood with anticoagulants 55%

Fluid portion of blood with out clotting factors

Chilled Specimen
ABG- Aterial Blood Gases

Sodium Citrate
Binds Calcium

Bruise, clotted blood underneath the skin

% of blood volume in the body that is RBC

Increased ratio of formed elements (such as RBC) to plasma

Light Sensitive

Chain of Custody
*purpose and procedure explained to pt
*pt consent form
*pt picture identification

Bilirubin, Vitamins C, K, and E
Protect from light

Immediately 1 hour to report

2-4 hours to report

Polymer gel/ clot activator

Carry blood that is high in oxygen

Blood Borne Pathogens
Pathogenic microorganisms that are present in the blood

Objects that can penetrate the skin such as needles, scalpels, broken glass, broken capillary tubes, or lancets.

The yellow fluid component of blood

White blood cell

Iron containing protien pigment present in red blood cells, it functions primarily to transport oxygen from the lungs to the tissue of the body


Red Blood Cell

Disease producing agents

Cessation of bleeding , formation of a clot (hemostasis)

Sharps Container
Fill only 80% of capacity to prevent accidental needle puncture

Housekeeping rule of thumb (Cheapest)
One cup of Bleach = 10 cups of water

70% alcohol

Standard precautions policy states that if there is a possibility of coming into contact with a patient’s blood, GLOVES ARE NECESSARY

Gauge of needle
The size of the needle at smallest is 27 and 16 at the largest

Tourniquet is placed
3-4 inches above the skin and should never be left on longer than one minute

A properly labeled tube has
Patient’s complete name,. id, number date and time sample obtained with initials

Limit of Sticks on a patient
If you miss twice trying for a blood draw you may not try again

Arterial Puncture
Done by physician only

Capillary Puncture
Puncture skin by lancet

Puncture to vein with the purpose of obtaining blood

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