Microbiology CH 4,5,6 – Flashcards
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| Bacterial cells could have any of the following appendages "except" |
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| Cilia |
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| Spirochetes have a twisting and flexing locomotion due to appendages called? |
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| Periplasmic flagella (axial filaments) |
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| The short, numerous appendages used by some bacterial cells for adhering to surfaces are called |
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| Fimbriae |
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| The transfer of genes during bacterial conjugation involves rigid, tubular appendages called? |
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| Sex pili |
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| The term that refers to the presence of flagella all over the cell surface is? |
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| peritrichous |
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| The outcome of the Graim stain is based on differences in the cell's |
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| Cell Wall |
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| The most immediate result of destruction of a cell's ribosome would be |
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| Protein synthesis would stop |
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| A bacterial cell exhibiting chemotaxis probably has |
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| Flagella |
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| Which structure protects bacteria from being phagocytized |
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| Capsule |
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| Peptidoglycan is a unique macromolecule found in bacterial |
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| Cell Walls |
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| All of the following structures contribute to the ability of pathogenic bacteria to cause disease EXCEPT |
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| Capsule |
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| A prokaryotic cell wall that has primarily peptidoglycan with small amounts of teichoic acid and lipoteichoic acid is |
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| Gram Positive |
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| A bacterial genus that has a waxy mycolic acid in the cell walls is |
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| Mycobacterium |
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| All of the following pertain to endotoxins EXCEPT |
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| Found in acid fast bacterial cell walls |
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| The difference in cell wall structure of Mycobacterium and Nocardia compared to the typical gram positive bacterial cell wall structure is? |
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| Predominance of unique, waxy, lipids |
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| Lipopolysaccharide is an important cell wall component of? |
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| Gram negative bacteria |
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| The site for most ATP synthesis in prokaryotic cells is the |
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| Cell membrane |
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| The function of bacterial endospores is |
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| Protection of genetic material during harsh conditions |
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| Chemical analysis of a bacterial cell structure detects calcium dipicolinic acid. what is the identity of this structure? |
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| Endospore |
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| Endospores are? |
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| Metabolically inactive Resistant to heat & chemical destruction Resistant to destruction by radiation Living Structures (all are correct) |
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| Bacterial endospores are not produced by |
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| Staphylococcus |
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| Which term is NOT used to describe bacterial cell shapes |
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| tetrad |
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| Serological analysis for bacterial identification typically involves using |
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| Specific antibodies to the bacterial cell antigens |
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| An important indicator of evolutionary relatedness is to determine |
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| Nitrogen base sequence of rRNA |
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| Which is mismatched? |
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| a) Gracilicutes - gram - cell walls b) Firmicutes - gram + cell walls c) Tenericutes - waxy acid fast cell walls d) Mendosicutes - archaea cell walls e) none of the above **The Answer is "C" |
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| The presence of thylakoids, phycocyanin, gas inclusions and systs would be associated with |
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| Cyanobacteria |
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| Archaea do not have the typical peptidoglycan structure found in bacteria cell walls, T/F |
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| False |
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| Bacteria in the genus Mycoplasma and bacteria called L-Forms lack cell walls, T/F |
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| True |
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| Gram negative bacteria do not have peptidoglycan in their cell walls, T/F |
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| False |
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| Hot carbol fushsin is the primary dye in the acid fast stain, T/F |
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| True |
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| The region between the bacterial cell membrane and the cell wall is called the outer membrane, T/F |
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| True |
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| The prokaryotic cell membrane is a site for many enzymes and metabolic reactions, T/F |
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| True |
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| Endospores of certain bacterial species can enter tissues in the human body, germinate, and cause an infectious disease, T/F |
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| True |
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| Iodine is the decolorizer in the Gram stain, T/F |
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| False |
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| If you observe rod shaped, pink cells on a slide that had just been gram stained, you can assume that their cell walls contain endotoxin, T/F |
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| False |
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| The term diplococci refers to an irregular cluster of spherical bacterial cells, T/F |
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| False |
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| Viruses have all of the following EXCEPT |
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| a) definite shape b) metabolism c) genes d) ability to infect host cells e) ultramicroscopic size **Answer is "B" Metabolism |
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| Host cells of viruses include |
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| a) human and other animals b) plants and fungi c) bacteria d) protozoa and algae e) all of the above **Answer is "E" all of the above |
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| The core of every virus particle always contains |
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| either DNA or RNA |
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| Classification of viruses into families involves determining all of the following characteristics EXCEPT |
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| Biochemical reactions |
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| Which of the following represents a virus family name? |
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| Herpesviridae |
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| Viruse capsids are made from subunits called |
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| Capsomeres |
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| Helical and icosahedral are terms used to describe the shapes of a virus |
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| Capsid |
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| All of the following pertain to virus envelopes EXCEPT |
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| Located between the capsid and nucleic acid |
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| Which of the following is NOT associated with every virus |
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| Envelope |
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| These structures are used by bacteriophages to attach to host cell receptors |
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| Tail fibers |
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| Which is incorrect about prophages |
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| Cause lysis of host cells |
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| T-Even phages |
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| Infect Escherichia coli cells |
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| The correct sequence of events in viral multiplication is |
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| Adsorption, penetration, replication, assembly, maturation, release |
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| The event that occurs in bacteriophage multiplication that does not occur in animal virus replication is |
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| Injection of the viral nucleic acid into the host cell |
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| Viruses acquire envelopes around their nucleocapsids during |
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| Release |
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| which of the following will NOT support viral cultivation |
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| Blood agar |
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| In general, most DNA viruses multiply in the host cell's _____, while most RNA viruses multiply in the host cell's _____ |
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| nucleus, cytoplasm |
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| Host range is limited by |
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| Type of host cell receptors on cell membrane |
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| The virus-induced, specific damage to the host cell that can be seen in a light microscope is called |
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| Plaques |
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| Visible, clear, well-defined patches in a monolayer of virus-infected cells in a culture are called |
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| Plaques |
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| Viral growth in bird embryos can cause discrete, opaque spots in the embryonic membranes called |
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| Pocks |
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| viruses that cause infection resulting in alternating periods of activity with symptoms and inactivity without symptoms are called |
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| latent |
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| Oncogenic viruses include all of the following EXCEPT |
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| measles virus |
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| Which of the following is a type of cytopathic effect? |
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| a) inclusions in the nucleus b) multinucleated giant cell c) inclusions in the cytoplasm d) cells round up e) all of the above **Answer is "E" all of the above |
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| Infectious protein particles are called |
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| Prions |
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| Infectious naked strands of RNA are called |
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| viroids |
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| Creutzfeld-Jacob disease is |
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| A spongiform encephalopathy of humans |
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| A naked virus does not have an envelope, T/F |
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| True |
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| viruses are ultramicroscopic because they range in size from 2?m to 450?m, T/F |
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| False |
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| Spikes are glycoproteins of the virus capsid, T/F |
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| False |
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| Prophages can be activated into viral replication and entering the lytic cycle, t/F |
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| True |
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| a specific animal virus has the ability to attach to and enter almost any animal host cell, T/F |
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| False |
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| Each virus is assigned to genus status based on its host, target tissue, and type of disease it causes, T/F |
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| True |
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| Bacteriophages do not undergo adsorption to specific host cell receptors prior to penetration, T/F |
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| False |
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| when a virus enters a host cell, the viral genes redirect the genetic and metabolic activities of the host cell, T/F |
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| True |
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| Viral spikes are inserted into the host cell membrane before budding or exocytosis, T/F |
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| False |
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| Viruses are used to produce vaccines for prevention of certain viral infections, T/F |
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| True |
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| One of the principal capsid shapes is a 20-sided figure with 12 evenly spaced corners referred to as an _______ capsid |
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| Icosahedral |
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| A _______ is the protein shell around the nucleic acid core of a virus |
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| Capsid |
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| Viruses that infect bacteria are specifically called ________ |
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| Phage |
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| Diagnosis of viral infections sometimes involves analyzing the patients blood for specific _________ that the immune system produced against the virus. |
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| Antibodies |
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| Two noncellular agents, smaller than viruses, are the infectious proteins called ________, and the infectious RNA strands called _______ |
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| prion, viroids |
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| During lysogeny, an inactive prophage state occurs when the viral DNA is inserted into the bacterial ____ ___________ |
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| Host chromosome |
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| A common method for cultivating viruses in the lab is to use in vitro systems called _______ cultures |
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| Cell |
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| If bacteria living in salty seawater were displaced to a freshwater environment, the cell structure that would prevent the cell from rupturing is |
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| Cell Walls |
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| The periplasmic space is? |
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| An important reaction site for substances entering and leaving the cell |
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| The bacterial chromosome |
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| a) is located in the cell membrane b) contains all the cell's plasmids c) is part of the nucleoid d) forms a single linear strand of DNA e) all choices are correct **Answer is "E" all choices are correct |
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| Which is mismatched |
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| a) ribosomes - protein synthesis b) inclusions - excess cell nutrients & materials c) plasmids - genes essential for growth and metabolism d) nucleoid - hereditary material e) cytoplasm - dense, gelatinous solution |
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| Magnetosomes are? |
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| Composed of magnetic iron oxide particles |
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| Which is mismatched? |
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| a) chlamydias-lack ability to independently complete grown & metabolism b) Green sulfur bacteria - photosynthetic anaerobes that use sulfur in metabolism c) Cyanobacteria - filamentous, gliding, thermophilic, photosynthetic bacteria d) Myxobacteria - gliding, fruiting, slime bacteria e) none are correct |
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| If during the gram stain procedure, the bacterial cell were viewed immediately after crystal violet was applied, gram positive cells would be purple but gram negative cells would be colorless, T/F |
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| False |