Micro II-A – Flashcards

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Acid-fast bacteria are identified by what staining technique
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Ziehl-Neelsen
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Spirochetes contain which morphological trait
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axial filaments (endoflagella)
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Corynebacterium diptheriae
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Gram +
Rod or Club shape
humans the only resevoir
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Diptheria method of disease
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diptheria toxin
A-B toxin
A- to cytosol
B- att. to vesicle membrane
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How Diptheria A-B toxin functions
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ADP-ribosylation of EF-2
Inactivates EF-2
Inhibiting Protein Synthesis
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Domains of diptheria toxin
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A- catalytic domain
B- binding domain
B- transport domain
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Bordetella pertussis
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Gram -
rod
aerobic
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Pertussis action
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adhesins att. to ciliated epi cells
pertusis toxin has 5 segments
-2,5 binding
-1 ADP-ribosylation
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pertussis ADP-ribosylation
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modifies adenylate cyclase
increased cAMP
inhibits neutrophile production
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Streptococci and Staphylcocci
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round
gram +
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Which bacteria is positive for the catalase test
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Staphylcocci
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Colonization of streptococci
colonization of staphylcocci
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string of pearls
cluster of grapes
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Which bacteria is positive for the coagulase test
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Staphylcoccus aureus
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Toxin of staphylcocci which can spread to whole body, via bloodstream
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exfolatin toxin
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How is toxic shock syndrome caused
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super-antigen
staph aureus toxin
Activates many many T/B cells
Increases IL-2 + TNF-a = SHOCK
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perioral erythema is characterized by what?
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a localized rash
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How does staph cause food poisoning
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Although the bact is killed by heat
Toxin is heat labile, and survives
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Besides having toxin, what is a virulence factor of staph aureus
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Protein A coat
-binds Fc receptor of IgG
-prevents Ab mediated host response
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The three groups of streptococcus are what, and are based on what
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alpha, beta, gamma
based on hemolysis
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What is the main pathogenic group of streptococci
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beta

hemolysins create a clearing for colonization

also A-group (for lancefield glyco-classification)

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Besides division based on hemolysis, what is another category of streptococci
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Cell wall polysaccharides
"Lancefield Groups" A-T
(A most virulent)
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necrotizing fascitis is characterized by: (bact, sympto)
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Invasive Group A Streptococci
Muscle/Fat destruction along facial plane
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Streptococci virulence
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M-protein has antiphag behavior
binds serum B-globulin factor H
this DEGRADES c3b
-M has many serotypes
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Most GI infections are characterized by what bacteria
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Gram -
Enterobacteriaceae
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E.coli O157:H7 refers to what?
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O- surface antigen O(LPS)
H- flagella antigen H
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Disease of shigella
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Dysentry
Bloody (+Mucus) Diarrhea
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What is similar between salmonella and e.coli
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they share ~90% of same DNA makeup
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Watery diahrrea characteristics
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Colonization of tract
production of toxin
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Colonization Factors I + II
-associated with bacteria
-function
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diarrhea causing e.coli
encoded on plasmid
enhance pili adherence to glycoproteins
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ETEC and Cholera toxin characteristics
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exotoxins which cause diarrhea
2 types:
-Heat labile (LT)
-Heat stable (ST)
5xB and 1xA subunits
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ETEC and Cholera Toxin mode
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The A-subunit ADP-ribosylates alpha subunit of G-protein
G-protein stims adenylate cyclase
cAMP produced
activates Protein Kinase
Pi's membrane, release of water/electro.
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Shigella produces ___ diarrhea by utilizing ____
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Bloody
Invasins (adhesins)
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How shigella's invasins work
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Bind to host integrins
Taken up by Phag, but break open vesicle
Multiply and spread
Mucosal cells die, inflamm. response
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Shigella and EHEC toxin and mode
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A-B
B subunit recognizes host
endocytosed
A cleaves N-glycosidic bond of 28s rRNA
Deactivation = no protein synthesis
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Typhoid is caused by ____
and characterized by ____
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salmonella
exists in blood, liver, monocytes
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Salmonella invasion method
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triggers actin rearrangement
causes pseudopods to engulf bact.
proliferation and breaking-open ("inv")
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Neisseria gonorrhoeae
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Gram -
Diploic (paired, like coffee bean)
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Bacteria characterized by having LOS (lipo-oligo-saccharide)
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Neisseria gonorrhoeae
-triggers inflammation
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Neisseria gonorrhoeae method
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tight adherence to mucosal cells
(like endocervical columnar epi)
PII/Opa directs this tight jct.
P1 protects bact. from PMNs
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Neisseria gonorrhoe avoidance of immune system
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serotypes- multiple Ag pili
phase variance- not all of its surface components turned on
IgA Protease- enzyme inacts. Ab
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Treponema pallidum
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Syphilis
spirochete
Needs darkfield or staining to see
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3 stages of syphilis
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1: lesion, chancre
2: mucosal membranes, blood
3: rare, musculoskeletal and organs
(only non-contagious stage)
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syphilis method
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surface coated with:
-IgG heavy chain
-MHC-I
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Chlamydiae trachomatis
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obligate intracellular pathogen
very small
small genome
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Chlamydia life cycle
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Small elementary body (EB)
-spore-like, infectious

Reticulate Body(RB)
-exists only in cell, proliferates
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Treatment of chlamydia
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azithro, doxy, erythro
-pregnant: ONLY azithro
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Helicobacter pylori
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Gram -
Rod, spiral shaped
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Helicobacter pylori disease and location of colonization
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Gastritis, duodenal ulcers
The acidic antrum of stomach
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Helicobacter pylori virulence
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Urease on surface, makes NH3: neutralizes
LPS inhibits glycosylation of mucus
-lipid A has low Pi, not seen as Ag
-LPS stims. pepsinogen, weakens walls
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People with Helicobacter pylori might have high levels of:
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IgG antibody
-because bact. causes high IL-8
-induces immune response
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Borrelia burgdorferi
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Lyme disease
Spirochete
axial filaments
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Borrelia burgdorferi mode of motility
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axial filaments (in periplasmic space)
-corkscrew movement
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Initially lyme disease seen as...
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Juvenile rheumatoid arthritis
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Mycobacterium tuberculosis
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acid-fast stain
mycolic acid (B-hydroxy F.A.)
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Potential carriers for Tb
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HIV/Aids
Compromised immunity
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Why is Tb easily spread?
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Pulmonary lesions easily ejected
Mycolic acid makes it resistant to drying when in air/dust
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Main cause of lung problems of Tb
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Inflammatory response releases TNF-a
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How Tb skin test works
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delayed hypersensitivity
injection with Tb protein fragments
(if body infected, then its Ab would elicit response)
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Treatment of Tb
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6 months (rifamp, isoniazid)
1st 2 months (pyrazinamide)
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Name 6 common Virulence Factors
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Adherence/colonization
Invasion of Host Cell/Tissues
Avoiding defenses
Toxins
Hydrolytic enzymes
Tissue damage (via immune response)
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General adherence is mediated by ___
Tightened adherence by ____
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pili/fimbriae
afimbrial adhesins
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Name two examples of afimbrial adhesins
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Gonorhea: PII
e.coli: Colonization Factors I, II
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Intra/Extra-cellular facultative bacteria often involved with which type of virulence
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Invasive
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Examples of avoiding defenses (virulence)
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Inhibit complement
Antiphag activity
Ag variation
Superantigen
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Bacteria inhibiting complement
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S.aureus:  hides surface Ag in capsule

meningococci:   coated by protein A (IgA)

S.pyogenes:   degrades c5A

salmonella/e.coli:   blocks MAC (LPS doesn't allow access)

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Example of endotoxin
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Gram -
LPS (A-in O-out)
Induces IL's and TNF-a
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A-B toxins and ADP-ribosylation
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cholera: g-protein, increased camp
diptheria- EF2, inactivates
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Where many virulence genes are located
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Plasmids and Phages
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A bacteria with chromosomally located virulence genes
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Cholera (for cholera toxin)
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Pathogenicity Island
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group of virulent genes on chromosome
distinct structural and functional units
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Common position of pathogenicity islands
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at a tRNA locus
(source for phage introduction)
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Possible explanation for similarity between cholera toxin + ETEC toxin
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horizontal gene transfer
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