Micro II-A – Flashcards
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Acid-fast bacteria are identified by what staining technique |
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Ziehl-Neelsen |
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Spirochetes contain which morphological trait |
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axial filaments (endoflagella) |
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Corynebacterium diptheriae |
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Gram + Rod or Club shape humans the only resevoir |
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Diptheria method of disease |
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diptheria toxin A-B toxin A- to cytosol B- att. to vesicle membrane |
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How Diptheria A-B toxin functions |
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ADP-ribosylation of EF-2 Inactivates EF-2 Inhibiting Protein Synthesis |
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Domains of diptheria toxin |
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A- catalytic domain B- binding domain B- transport domain |
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Bordetella pertussis |
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Gram - rod aerobic |
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Pertussis action |
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adhesins att. to ciliated epi cells pertusis toxin has 5 segments -2,5 binding -1 ADP-ribosylation |
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pertussis ADP-ribosylation |
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modifies adenylate cyclase increased cAMP inhibits neutrophile production |
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Streptococci and Staphylcocci |
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round gram + |
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Which bacteria is positive for the catalase test |
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Staphylcocci |
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Colonization of streptococci colonization of staphylcocci |
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string of pearls cluster of grapes |
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Which bacteria is positive for the coagulase test |
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Staphylcoccus aureus |
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Toxin of staphylcocci which can spread to whole body, via bloodstream |
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exfolatin toxin |
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How is toxic shock syndrome caused |
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super-antigen staph aureus toxin Activates many many T/B cells Increases IL-2 + TNF-a = SHOCK |
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perioral erythema is characterized by what? |
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a localized rash |
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How does staph cause food poisoning |
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Although the bact is killed by heat Toxin is heat labile, and survives |
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Besides having toxin, what is a virulence factor of staph aureus |
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Protein A coat -binds Fc receptor of IgG -prevents Ab mediated host response |
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The three groups of streptococcus are what, and are based on what |
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alpha, beta, gamma based on hemolysis |
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What is the main pathogenic group of streptococci |
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beta hemolysins create a clearing for colonization also A-group (for lancefield glyco-classification) |
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Besides division based on hemolysis, what is another category of streptococci |
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Cell wall polysaccharides "Lancefield Groups" A-T (A most virulent) |
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necrotizing fascitis is characterized by: (bact, sympto) |
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Invasive Group A Streptococci Muscle/Fat destruction along facial plane |
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Streptococci virulence |
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M-protein has antiphag behavior binds serum B-globulin factor H this DEGRADES c3b -M has many serotypes |
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Most GI infections are characterized by what bacteria |
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Gram - Enterobacteriaceae |
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E.coli O157:H7 refers to what? |
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O- surface antigen O(LPS) H- flagella antigen H |
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Disease of shigella |
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Dysentry Bloody (+Mucus) Diarrhea |
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What is similar between salmonella and e.coli |
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they share ~90% of same DNA makeup |
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Watery diahrrea characteristics |
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Colonization of tract production of toxin |
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Colonization Factors I + II -associated with bacteria -function |
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diarrhea causing e.coli encoded on plasmid enhance pili adherence to glycoproteins |
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ETEC and Cholera toxin characteristics |
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exotoxins which cause diarrhea 2 types: -Heat labile (LT) -Heat stable (ST) 5xB and 1xA subunits |
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ETEC and Cholera Toxin mode |
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The A-subunit ADP-ribosylates alpha subunit of G-protein G-protein stims adenylate cyclase cAMP produced activates Protein Kinase Pi's membrane, release of water/electro. |
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Shigella produces ___ diarrhea by utilizing ____ |
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Bloody Invasins (adhesins) |
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How shigella's invasins work |
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Bind to host integrins Taken up by Phag, but break open vesicle Multiply and spread Mucosal cells die, inflamm. response |
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Shigella and EHEC toxin and mode |
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A-B B subunit recognizes host endocytosed A cleaves N-glycosidic bond of 28s rRNA Deactivation = no protein synthesis |
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Typhoid is caused by ____ and characterized by ____ |
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salmonella exists in blood, liver, monocytes |
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Salmonella invasion method |
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triggers actin rearrangement causes pseudopods to engulf bact. proliferation and breaking-open ("inv") |
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Neisseria gonorrhoeae |
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Gram - Diploic (paired, like coffee bean) |
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Bacteria characterized by having LOS (lipo-oligo-saccharide) |
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Neisseria gonorrhoeae -triggers inflammation |
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Neisseria gonorrhoeae method |
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tight adherence to mucosal cells (like endocervical columnar epi) PII/Opa directs this tight jct. P1 protects bact. from PMNs |
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Neisseria gonorrhoe avoidance of immune system |
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serotypes- multiple Ag pili phase variance- not all of its surface components turned on IgA Protease- enzyme inacts. Ab |
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Treponema pallidum |
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Syphilis spirochete Needs darkfield or staining to see |
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3 stages of syphilis |
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1: lesion, chancre 2: mucosal membranes, blood 3: rare, musculoskeletal and organs (only non-contagious stage) |
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syphilis method |
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surface coated with: -IgG heavy chain -MHC-I |
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Chlamydiae trachomatis |
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obligate intracellular pathogen very small small genome |
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Chlamydia life cycle |
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Small elementary body (EB) -spore-like, infectious Reticulate Body(RB) -exists only in cell, proliferates |
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Treatment of chlamydia |
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azithro, doxy, erythro -pregnant: ONLY azithro |
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Helicobacter pylori |
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Gram - Rod, spiral shaped |
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Helicobacter pylori disease and location of colonization |
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Gastritis, duodenal ulcers The acidic antrum of stomach |
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Helicobacter pylori virulence |
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Urease on surface, makes NH3: neutralizes LPS inhibits glycosylation of mucus -lipid A has low Pi, not seen as Ag -LPS stims. pepsinogen, weakens walls |
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People with Helicobacter pylori might have high levels of: |
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IgG antibody -because bact. causes high IL-8 -induces immune response |
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Borrelia burgdorferi |
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Lyme disease Spirochete axial filaments |
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Borrelia burgdorferi mode of motility |
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axial filaments (in periplasmic space) -corkscrew movement |
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Initially lyme disease seen as... |
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Juvenile rheumatoid arthritis |
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Mycobacterium tuberculosis |
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acid-fast stain mycolic acid (B-hydroxy F.A.) |
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Potential carriers for Tb |
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HIV/Aids Compromised immunity |
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Why is Tb easily spread? |
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Pulmonary lesions easily ejected Mycolic acid makes it resistant to drying when in air/dust |
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Main cause of lung problems of Tb |
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Inflammatory response releases TNF-a |
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How Tb skin test works |
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delayed hypersensitivity injection with Tb protein fragments (if body infected, then its Ab would elicit response) |
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Treatment of Tb |
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6 months (rifamp, isoniazid) 1st 2 months (pyrazinamide) |
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Name 6 common Virulence Factors |
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Adherence/colonization Invasion of Host Cell/Tissues Avoiding defenses Toxins Hydrolytic enzymes Tissue damage (via immune response) |
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General adherence is mediated by ___ Tightened adherence by ____ |
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pili/fimbriae afimbrial adhesins |
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Name two examples of afimbrial adhesins |
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Gonorhea: PII e.coli: Colonization Factors I, II |
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Intra/Extra-cellular facultative bacteria often involved with which type of virulence |
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Invasive |
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Examples of avoiding defenses (virulence) |
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Inhibit complement Antiphag activity Ag variation Superantigen |
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Bacteria inhibiting complement |
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S.aureus: hides surface Ag in capsule meningococci: coated by protein A (IgA) S.pyogenes: degrades c5A salmonella/e.coli: blocks MAC (LPS doesn't allow access) |
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Example of endotoxin |
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Gram - LPS (A-in O-out) Induces IL's and TNF-a |
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A-B toxins and ADP-ribosylation |
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cholera: g-protein, increased camp diptheria- EF2, inactivates |
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Where many virulence genes are located |
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Plasmids and Phages |
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A bacteria with chromosomally located virulence genes |
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Cholera (for cholera toxin) |
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Pathogenicity Island |
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group of virulent genes on chromosome distinct structural and functional units |
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Common position of pathogenicity islands |
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at a tRNA locus (source for phage introduction) |
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Possible explanation for similarity between cholera toxin + ETEC toxin |
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horizontal gene transfer |