MCB 100 Chapter 5 – Flashcards

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Metabolism
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Sum of biochemical reactions within the cells of an organism
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Catabolism
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Breaks down molecules and releases energy
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Anabolism
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Synthesizes molecules and uses energy
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Precursor metabolites
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Produced in catabolic reactions and used to synthesize all organic compounds
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Reduction Reactions
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Electrons are added to reaction - +2H or -O
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Oxidized molecule
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Molecule that donates and electron; if hydrogen – reaction is called dehydrogenation.
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Photoautotrophy
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Energy from light, carbon from CO2
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Chemoautotrophy
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Energy from inorganic redox reaction, carbon from CO2
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Important elections carrier molecules
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NAD+, NADP+, FAD
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Phosphorylation
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Addition of phosphate to a molecule, used to form ATP
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Ways cells make ATP
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1. H+ -ATPase
2. Substrate-level phosphorylation
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Substrate-level phosphorylation
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Requires sacrifice of a chemical bond that has higher energy than the high energy bond of ATP
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Ways cells use ATP
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Substrate activation (used to attach amino acids to tRNA molecules)
power cellular motion (flagella rotation, muscle fibers contract)
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Chemoheterotrophs
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Make energy via redox reactions
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Catalysts
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Increase rates of chemical reaction and are not permanently changed in the process
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Enzymes
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Organic catalysts, named for their substrates (molecules on which they act) – most are proteins
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Simple proteins
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Chain of amino acids
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Conjugated Enzyme
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Protein plus additional organic or inorganic molecules that are closely associated with the protein
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Apoenzymes
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Portions of enzymes that require one or more cofactors (or coenzymes if organic)
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Cofactor
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Small molecule that binds to an enzyme and helps to produce the catalytic activity
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Activation energy
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Amount of energy required to initiate a chemical reaction
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Active sites
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Places of the enzyme that substrates fit into in order to catalyze the reaction
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ATP
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3 phosphate groups on adenosine, energy molecule of cells, energy released when it loses a phosphate to form ADP + free phosphate
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Competitive inhibitors
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Block active sites and block enzyme activity
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Glycolysis
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Splitting of a glucose molecule in a three stage, 10 step process – end result being two molecules of pyruvic acid and +2 ATP, +2 NADH molecules, costs 2 ATPs to start
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Kreb’s Cycle
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Series of reactions that result in the complete oxidation of (2) pyruvate to (6) CO2
8 molecules NAD+ reduced to NADH
2 ATPs are produced by substrate level phosphorylation
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