Microbiology Final Exam Flashcard

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G eneral characteristics of fungi
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Chitin in cell wall, ergosterol in cell membrane, reproduction by means of spores, no chlorophyll, heterotrophic and not susceptible to antibacterial agents.
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Explain the purpose of cyclohexiamide in fungal media
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Cyclohexiamide is an antibiotic for inhibiting protein synthesis of saprobic fungi on sabouraud dextrose agar
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Germ tube
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(botany) a slender tubular outgrowth from a spore in germination.


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KOH wet prep
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KOH dissolves human cellular elements and debris, which allows for easier visualization of fungal elements
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Calcoflour white prep
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Calcoflour binds to chitin in fungal cell wall, fluorescence occurs at 400nm, fungal elements fluoresce blue-white
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In vitro hair penetration test
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some fungi will penetrate the hair shaft, Trichophyton mentagrophytes is positive, Trichophyton rubrum is negative
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Tease mount
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take mold from agar plate, and put it on a slide with a drop of lactophenol blue, used to identify morphologic structures of mold
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Slide culture
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cut a small block of sterile agar, place on a sterile slide in a huminidity chamber, inoculate the four corners of the agar with fungi and apply a coverslip to the top of the agar. Ater incubation remove coverslip and palce on a new slide with a drop of lactopenal blue, observe for morphologic characteristics.
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Blastoconidia
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asexual reproduction of yeast, budding yeast cells from weakened out-pouching of cell wall
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Conidiophore
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specialized hyphal structure that serves as a stalk on which conidia are formed
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Mycelium
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mould characteristic, growing hyphae that become intertwined to form a loose network that can penetrate the substrate to obtain necessary nutrients
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Psuedohypha
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yeast buds elongate, fail to dissociate, and form subsequent buds, looks like a link of sausage, cell wall constrictions rather than septations
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Rhizoid
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root-like structures that appear and anchor the organism to the agar surface, used in identification of zygomycetes
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Saprophyte
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living on dead or decayed organic mater, characteristic of most of the fungal organisms that cause human disease
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Septa
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cross walls in hyphae
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Sporangiophore
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supporting structure at which each sporangium is formed at the tip of, usually connected to one another by septate hyphae
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Spore
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A minute, typically one-celled, reproductive unit capable of giving rise to a new individual without sexual fusion
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Dematiaceous
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presence of pigmentation within the hyphae of molds
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Hyaline
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absence of pigmentation within the hyphae of molds
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Dimorphism in fungi
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can grow as yeast at body temp and as mould at room temp
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Clinical significance of dimorphism-
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allows organism to survive and infect humans inside their bodies as opposed to subcutaneous
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Dimorphic fungi
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Histoplasma capsulatum, Blastomyces dermatitidis, Coccidioides immitis, paracoccidiodes brasiliensis, Sporothrix schenckii, and Penicillium marneffei
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Mycoses
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fungal infection that causes disease
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Mycotoxicoses
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toxic secondary disease caused by toxin from fungi
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Mycetoma
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usually on hands and feet, chronic infection characterized by swollen tumor-like lesions that yield granular pus through draining sinuses, the granules are masses of compact mycelia
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Tinea
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infections of the skin or nails caused by dermatophyte fungi, outer ring is active, progressing infection, with central healing within the ring
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Chromoblastomycosis
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subcutaneous mycoses in which there is a localized cutaneous and subcutaneous infection where the tissue turns into rounded sclerotic bodies, called copper pennies
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Phaehyphomycosis
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term to describe any infection caused by a dematiacious organism, can be subcutaneous, localized, or systemic infections
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How do you tell a yeast from a mould?
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Yeast are single vegatative cells called blastoconidia, while moulds are filamentous fungi that can have mycelium, hyphae and rhizoids
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Conidia
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asexual reproduction that occurs on the side or end of hyphae or conidiophore
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Macroconidium
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larger of 2 types of conidia in a fungus that produces 2 sizes of conidia
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Microconidium
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smaller of 2 types of conidia in a fungus that produces 2 sizes of conidia
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Annellide
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a cell that produces & extrudes conidia; tapers & lengthens with each condidum
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Arthroconidium
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an asexual spore formed by the breaking of hyphae at the point of septation
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Philalide
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a cell that produces & extrudes conidia; does not taper or lengthen with each condidium
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Sporandiospore
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asexual spore produces within a sporangium
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Sproangium
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a closed sac-like structure in which asexual spores are formed by cleavage
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Zygomycota
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sexual reproduction occurs with the production of zygospores
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Type of Zygomycota
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Mucor, Rhizopus, Absidia
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Ascomycota
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sexual spores produced within a sac-like structure knowns as an ascus
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Types of Ascomycota
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Microsporum, Trichophyton, Pseudallescheria
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Basidiomycota
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sexual spore formed on a structure known as a basidium
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Types of Basidiomycota
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Filobasidiella neoformans (Cryptococcus neoformans)
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Fungi Imperfecti
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no known sexual mode of reproduction
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Superficial mycoses
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confined to the outermost layer of skin or hair
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Cutaneous mycoses
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affect the keratinized layer of skin, hair or nails
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Subcutaneous mycoses
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involve deeper skin layers, including muscle & connective tissue
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Systemic mycoses
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affect internal organs or deep tissues of the body
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Types of specimens (8)
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Hair
Skin
Nails
Blood & bone marrow
CSF
Abscess fluids & wound exudates
Respiratory specimens
Urogenital & fecal specimens
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Ways to perform direct microscopic examination (4)
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KOH preparations
KOH with calcofluor white
India Ink
Tissue stains
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Antibiotics used on media
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Chloramphenicol (bacteria)
Cyclohexamide (saprobic fungi)
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Incubation for fungal media
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25-30 C for 4 weeks
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