Microbiology Final Exam Flashcard
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G eneral characteristics of fungi |
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Chitin in cell wall, ergosterol in cell membrane, reproduction by means of spores, no chlorophyll, heterotrophic and not susceptible to antibacterial agents. |
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Explain the purpose of cyclohexiamide in fungal media |
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Cyclohexiamide is an antibiotic for inhibiting protein synthesis of saprobic fungi on sabouraud dextrose agar |
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Germ tube |
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(botany) a slender tubular outgrowth from a spore in germination. |
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KOH wet prep |
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KOH dissolves human cellular elements and debris, which allows for easier visualization of fungal elements |
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Calcoflour white prep |
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Calcoflour binds to chitin in fungal cell wall, fluorescence occurs at 400nm, fungal elements fluoresce blue-white |
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In vitro hair penetration test |
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some fungi will penetrate the hair shaft, Trichophyton mentagrophytes is positive, Trichophyton rubrum is negative |
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Tease mount |
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take mold from agar plate, and put it on a slide with a drop of lactophenol blue, used to identify morphologic structures of mold |
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Slide culture |
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cut a small block of sterile agar, place on a sterile slide in a huminidity chamber, inoculate the four corners of the agar with fungi and apply a coverslip to the top of the agar. Ater incubation remove coverslip and palce on a new slide with a drop of lactopenal blue, observe for morphologic characteristics. |
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Blastoconidia |
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asexual reproduction of yeast, budding yeast cells from weakened out-pouching of cell wall |
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Conidiophore |
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specialized hyphal structure that serves as a stalk on which conidia are formed |
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Mycelium |
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mould characteristic, growing hyphae that become intertwined to form a loose network that can penetrate the substrate to obtain necessary nutrients |
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Psuedohypha |
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yeast buds elongate, fail to dissociate, and form subsequent buds, looks like a link of sausage, cell wall constrictions rather than septations |
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Rhizoid |
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root-like structures that appear and anchor the organism to the agar surface, used in identification of zygomycetes |
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Saprophyte |
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living on dead or decayed organic mater, characteristic of most of the fungal organisms that cause human disease |
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Septa |
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cross walls in hyphae |
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Sporangiophore |
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supporting structure at which each sporangium is formed at the tip of, usually connected to one another by septate hyphae |
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Spore |
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A minute, typically one-celled, reproductive unit capable of giving rise to a new individual without sexual fusion |
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Dematiaceous |
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presence of pigmentation within the hyphae of molds |
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Hyaline |
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absence of pigmentation within the hyphae of molds |
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Dimorphism in fungi |
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can grow as yeast at body temp and as mould at room temp |
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Clinical significance of dimorphism- |
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allows organism to survive and infect humans inside their bodies as opposed to subcutaneous |
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Dimorphic fungi |
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Histoplasma capsulatum, Blastomyces dermatitidis, Coccidioides immitis, paracoccidiodes brasiliensis, Sporothrix schenckii, and Penicillium marneffei |
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Mycoses |
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fungal infection that causes disease |
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Mycotoxicoses |
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toxic secondary disease caused by toxin from fungi |
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Mycetoma |
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usually on hands and feet, chronic infection characterized by swollen tumor-like lesions that yield granular pus through draining sinuses, the granules are masses of compact mycelia |
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Tinea |
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infections of the skin or nails caused by dermatophyte fungi, outer ring is active, progressing infection, with central healing within the ring |
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Chromoblastomycosis |
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subcutaneous mycoses in which there is a localized cutaneous and subcutaneous infection where the tissue turns into rounded sclerotic bodies, called copper pennies |
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Phaehyphomycosis |
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term to describe any infection caused by a dematiacious organism, can be subcutaneous, localized, or systemic infections |
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How do you tell a yeast from a mould? |
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Yeast are single vegatative cells called blastoconidia, while moulds are filamentous fungi that can have mycelium, hyphae and rhizoids |
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Conidia |
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asexual reproduction that occurs on the side or end of hyphae or conidiophore |
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Macroconidium |
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larger of 2 types of conidia in a fungus that produces 2 sizes of conidia |
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Microconidium |
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smaller of 2 types of conidia in a fungus that produces 2 sizes of conidia |
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Annellide |
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a cell that produces & extrudes conidia; tapers & lengthens with each condidum |
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Arthroconidium |
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an asexual spore formed by the breaking of hyphae at the point of septation |
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Philalide |
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a cell that produces & extrudes conidia; does not taper or lengthen with each condidium |
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Sporandiospore |
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asexual spore produces within a sporangium |
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Sproangium |
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a closed sac-like structure in which asexual spores are formed by cleavage |
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Zygomycota |
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sexual reproduction occurs with the production of zygospores |
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Type of Zygomycota |
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Mucor, Rhizopus, Absidia |
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Ascomycota |
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sexual spores produced within a sac-like structure knowns as an ascus |
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Types of Ascomycota |
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Microsporum, Trichophyton, Pseudallescheria |
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Basidiomycota |
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sexual spore formed on a structure known as a basidium |
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Types of Basidiomycota |
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Filobasidiella neoformans (Cryptococcus neoformans) |
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Fungi Imperfecti |
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no known sexual mode of reproduction |
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Superficial mycoses |
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confined to the outermost layer of skin or hair |
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Cutaneous mycoses |
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affect the keratinized layer of skin, hair or nails |
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Subcutaneous mycoses |
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involve deeper skin layers, including muscle & connective tissue |
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Systemic mycoses |
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affect internal organs or deep tissues of the body |
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Types of specimens (8) |
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Hair Skin Nails Blood & bone marrow CSF Abscess fluids & wound exudates Respiratory specimens Urogenital & fecal specimens |
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Ways to perform direct microscopic examination (4) |
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KOH preparations KOH with calcofluor white India Ink Tissue stains |
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Antibiotics used on media |
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Chloramphenicol (bacteria) Cyclohexamide (saprobic fungi) |
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Incubation for fungal media |
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25-30 C for 4 weeks |