Microbiology Final Exam Flashcard

question
G eneral characteristics of fungi
answer
Chitin in cell wall, ergosterol in cell membrane, reproduction by means of spores, no chlorophyll, heterotrophic and not susceptible to antibacterial agents.
question
Explain the purpose of cyclohexiamide in fungal media
answer
Cyclohexiamide is an antibiotic for inhibiting protein synthesis of saprobic fungi on sabouraud dextrose agar
question
Germ tube
answer

(botany) a slender tubular outgrowth from a spore in germination.


question
KOH wet prep
answer
KOH dissolves human cellular elements and debris, which allows for easier visualization of fungal elements
question
Calcoflour white prep
answer
Calcoflour binds to chitin in fungal cell wall, fluorescence occurs at 400nm, fungal elements fluoresce blue-white
question
In vitro hair penetration test
answer
some fungi will penetrate the hair shaft, Trichophyton mentagrophytes is positive, Trichophyton rubrum is negative
question
Tease mount
answer
take mold from agar plate, and put it on a slide with a drop of lactophenol blue, used to identify morphologic structures of mold
question
Slide culture
answer
cut a small block of sterile agar, place on a sterile slide in a huminidity chamber, inoculate the four corners of the agar with fungi and apply a coverslip to the top of the agar. Ater incubation remove coverslip and palce on a new slide with a drop of lactopenal blue, observe for morphologic characteristics.
question
Blastoconidia
answer
asexual reproduction of yeast, budding yeast cells from weakened out-pouching of cell wall
question
Conidiophore
answer
specialized hyphal structure that serves as a stalk on which conidia are formed
question
Mycelium
answer
mould characteristic, growing hyphae that become intertwined to form a loose network that can penetrate the substrate to obtain necessary nutrients
question
Psuedohypha
answer
yeast buds elongate, fail to dissociate, and form subsequent buds, looks like a link of sausage, cell wall constrictions rather than septations
question
Rhizoid
answer
root-like structures that appear and anchor the organism to the agar surface, used in identification of zygomycetes
question
Saprophyte
answer
living on dead or decayed organic mater, characteristic of most of the fungal organisms that cause human disease
question
Septa
answer
cross walls in hyphae
question
Sporangiophore
answer
supporting structure at which each sporangium is formed at the tip of, usually connected to one another by septate hyphae
question
Spore
answer
A minute, typically one-celled, reproductive unit capable of giving rise to a new individual without sexual fusion
question
Dematiaceous
answer
presence of pigmentation within the hyphae of molds
question
Hyaline
answer
absence of pigmentation within the hyphae of molds
question
Dimorphism in fungi
answer
can grow as yeast at body temp and as mould at room temp
question
Clinical significance of dimorphism-
answer
allows organism to survive and infect humans inside their bodies as opposed to subcutaneous
question
Dimorphic fungi
answer
Histoplasma capsulatum, Blastomyces dermatitidis, Coccidioides immitis, paracoccidiodes brasiliensis, Sporothrix schenckii, and Penicillium marneffei
question
Mycoses
answer
fungal infection that causes disease
question
Mycotoxicoses
answer
toxic secondary disease caused by toxin from fungi
question
Mycetoma
answer
usually on hands and feet, chronic infection characterized by swollen tumor-like lesions that yield granular pus through draining sinuses, the granules are masses of compact mycelia
question
Tinea
answer
infections of the skin or nails caused by dermatophyte fungi, outer ring is active, progressing infection, with central healing within the ring
question
Chromoblastomycosis
answer
subcutaneous mycoses in which there is a localized cutaneous and subcutaneous infection where the tissue turns into rounded sclerotic bodies, called copper pennies
question
Phaehyphomycosis
answer
term to describe any infection caused by a dematiacious organism, can be subcutaneous, localized, or systemic infections
question
How do you tell a yeast from a mould?
answer
Yeast are single vegatative cells called blastoconidia, while moulds are filamentous fungi that can have mycelium, hyphae and rhizoids
question
Conidia
answer
asexual reproduction that occurs on the side or end of hyphae or conidiophore
question
Macroconidium
answer
larger of 2 types of conidia in a fungus that produces 2 sizes of conidia
question
Microconidium
answer
smaller of 2 types of conidia in a fungus that produces 2 sizes of conidia
question
Annellide
answer
a cell that produces & extrudes conidia; tapers & lengthens with each condidum
question
Arthroconidium
answer
an asexual spore formed by the breaking of hyphae at the point of septation
question
Philalide
answer
a cell that produces & extrudes conidia; does not taper or lengthen with each condidium
question
Sporandiospore
answer
asexual spore produces within a sporangium
question
Sproangium
answer
a closed sac-like structure in which asexual spores are formed by cleavage
question
Zygomycota
answer
sexual reproduction occurs with the production of zygospores
question
Type of Zygomycota
answer
Mucor, Rhizopus, Absidia
question
Ascomycota
answer
sexual spores produced within a sac-like structure knowns as an ascus
question
Types of Ascomycota
answer
Microsporum, Trichophyton, Pseudallescheria
question
Basidiomycota
answer
sexual spore formed on a structure known as a basidium
question
Types of Basidiomycota
answer
Filobasidiella neoformans (Cryptococcus neoformans)
question
Fungi Imperfecti
answer
no known sexual mode of reproduction
question
Superficial mycoses
answer
confined to the outermost layer of skin or hair
question
Cutaneous mycoses
answer
affect the keratinized layer of skin, hair or nails
question
Subcutaneous mycoses
answer
involve deeper skin layers, including muscle & connective tissue
question
Systemic mycoses
answer
affect internal organs or deep tissues of the body
question
Types of specimens (8)
answer
Hair
Skin
Nails
Blood & bone marrow
CSF
Abscess fluids & wound exudates
Respiratory specimens
Urogenital & fecal specimens
question
Ways to perform direct microscopic examination (4)
answer
KOH preparations
KOH with calcofluor white
India Ink
Tissue stains
question
Antibiotics used on media
answer
Chloramphenicol (bacteria)
Cyclohexamide (saprobic fungi)
question
Incubation for fungal media
answer
25-30 C for 4 weeks
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question
G eneral characteristics of fungi
answer
Chitin in cell wall, ergosterol in cell membrane, reproduction by means of spores, no chlorophyll, heterotrophic and not susceptible to antibacterial agents.
question
Explain the purpose of cyclohexiamide in fungal media
answer
Cyclohexiamide is an antibiotic for inhibiting protein synthesis of saprobic fungi on sabouraud dextrose agar
question
Germ tube
answer

(botany) a slender tubular outgrowth from a spore in germination.


question
KOH wet prep
answer
KOH dissolves human cellular elements and debris, which allows for easier visualization of fungal elements
question
Calcoflour white prep
answer
Calcoflour binds to chitin in fungal cell wall, fluorescence occurs at 400nm, fungal elements fluoresce blue-white
question
In vitro hair penetration test
answer
some fungi will penetrate the hair shaft, Trichophyton mentagrophytes is positive, Trichophyton rubrum is negative
question
Tease mount
answer
take mold from agar plate, and put it on a slide with a drop of lactophenol blue, used to identify morphologic structures of mold
question
Slide culture
answer
cut a small block of sterile agar, place on a sterile slide in a huminidity chamber, inoculate the four corners of the agar with fungi and apply a coverslip to the top of the agar. Ater incubation remove coverslip and palce on a new slide with a drop of lactopenal blue, observe for morphologic characteristics.
question
Blastoconidia
answer
asexual reproduction of yeast, budding yeast cells from weakened out-pouching of cell wall
question
Conidiophore
answer
specialized hyphal structure that serves as a stalk on which conidia are formed
question
Mycelium
answer
mould characteristic, growing hyphae that become intertwined to form a loose network that can penetrate the substrate to obtain necessary nutrients
question
Psuedohypha
answer
yeast buds elongate, fail to dissociate, and form subsequent buds, looks like a link of sausage, cell wall constrictions rather than septations
question
Rhizoid
answer
root-like structures that appear and anchor the organism to the agar surface, used in identification of zygomycetes
question
Saprophyte
answer
living on dead or decayed organic mater, characteristic of most of the fungal organisms that cause human disease
question
Septa
answer
cross walls in hyphae
question
Sporangiophore
answer
supporting structure at which each sporangium is formed at the tip of, usually connected to one another by septate hyphae
question
Spore
answer
A minute, typically one-celled, reproductive unit capable of giving rise to a new individual without sexual fusion
question
Dematiaceous
answer
presence of pigmentation within the hyphae of molds
question
Hyaline
answer
absence of pigmentation within the hyphae of molds
question
Dimorphism in fungi
answer
can grow as yeast at body temp and as mould at room temp
question
Clinical significance of dimorphism-
answer
allows organism to survive and infect humans inside their bodies as opposed to subcutaneous
question
Dimorphic fungi
answer
Histoplasma capsulatum, Blastomyces dermatitidis, Coccidioides immitis, paracoccidiodes brasiliensis, Sporothrix schenckii, and Penicillium marneffei
question
Mycoses
answer
fungal infection that causes disease
question
Mycotoxicoses
answer
toxic secondary disease caused by toxin from fungi
question
Mycetoma
answer
usually on hands and feet, chronic infection characterized by swollen tumor-like lesions that yield granular pus through draining sinuses, the granules are masses of compact mycelia
question
Tinea
answer
infections of the skin or nails caused by dermatophyte fungi, outer ring is active, progressing infection, with central healing within the ring
question
Chromoblastomycosis
answer
subcutaneous mycoses in which there is a localized cutaneous and subcutaneous infection where the tissue turns into rounded sclerotic bodies, called copper pennies
question
Phaehyphomycosis
answer
term to describe any infection caused by a dematiacious organism, can be subcutaneous, localized, or systemic infections
question
How do you tell a yeast from a mould?
answer
Yeast are single vegatative cells called blastoconidia, while moulds are filamentous fungi that can have mycelium, hyphae and rhizoids
question
Conidia
answer
asexual reproduction that occurs on the side or end of hyphae or conidiophore
question
Macroconidium
answer
larger of 2 types of conidia in a fungus that produces 2 sizes of conidia
question
Microconidium
answer
smaller of 2 types of conidia in a fungus that produces 2 sizes of conidia
question
Annellide
answer
a cell that produces & extrudes conidia; tapers & lengthens with each condidum
question
Arthroconidium
answer
an asexual spore formed by the breaking of hyphae at the point of septation
question
Philalide
answer
a cell that produces & extrudes conidia; does not taper or lengthen with each condidium
question
Sporandiospore
answer
asexual spore produces within a sporangium
question
Sproangium
answer
a closed sac-like structure in which asexual spores are formed by cleavage
question
Zygomycota
answer
sexual reproduction occurs with the production of zygospores
question
Type of Zygomycota
answer
Mucor, Rhizopus, Absidia
question
Ascomycota
answer
sexual spores produced within a sac-like structure knowns as an ascus
question
Types of Ascomycota
answer
Microsporum, Trichophyton, Pseudallescheria
question
Basidiomycota
answer
sexual spore formed on a structure known as a basidium
question
Types of Basidiomycota
answer
Filobasidiella neoformans (Cryptococcus neoformans)
question
Fungi Imperfecti
answer
no known sexual mode of reproduction
question
Superficial mycoses
answer
confined to the outermost layer of skin or hair
question
Cutaneous mycoses
answer
affect the keratinized layer of skin, hair or nails
question
Subcutaneous mycoses
answer
involve deeper skin layers, including muscle & connective tissue
question
Systemic mycoses
answer
affect internal organs or deep tissues of the body
question
Types of specimens (8)
answer
Hair
Skin
Nails
Blood & bone marrow
CSF
Abscess fluids & wound exudates
Respiratory specimens
Urogenital & fecal specimens
question
Ways to perform direct microscopic examination (4)
answer
KOH preparations
KOH with calcofluor white
India Ink
Tissue stains
question
Antibiotics used on media
answer
Chloramphenicol (bacteria)
Cyclohexamide (saprobic fungi)
question
Incubation for fungal media
answer
25-30 C for 4 weeks
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