Enzymes of Glycolysis – Flashcards

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question
What is the first enzyme used in Glycolysis?
answer
Hexokinase
question
What does Hexokinase do?
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Takes an ATP and makes an ADP.  Adds a phosphate group to the 6th carbon of glucose, creating Glucose 6-phosphate
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What is the second enzyme in Glycolysis?
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Phosphoglucose Isomerase
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What does phosphoglucose isomerase do?
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Re-arranges molecules from the 1st and 2nd carbon groups, creating Fructose 6-phosphate
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What is the third enzyme in Gycolysis?
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Phosphofructokinase
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What does phosphofructosekinase do?
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Takes an ATP and makes an ADP.  Adds a phosphate group to the first carbon group creating Fructose 1,6-biphosphate.
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What is the fourth enzyme in Glycolysis?
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Aldolase
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What does Aldolase do?
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Splits the fructose 1,6 biphosphate into 2 compounds.  Dihydroxyacetone Phosphate and Glycereldahyde 3-phosphate.
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What is the fifth enzyme in Glycolysis?
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Triose Phosphate Isomerase
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What does Triose Phosphate Isomerase do?
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Rearranges molecules in Dihydroxyacetone Phosphate into Glyceraldehyde 3-phosphate
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What is the 6th enzyme in Glycolysis?
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Glyceraldehyde 3-phosphate dehydrogenase
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What does Glyceraldehyde 3-phosphate dehydrogenase do?
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Takes a Phosphate group and an NAD to reduce the NAD to NADH taking 1 hydrogen and convert the Glyceraldehyde 3-phosphate to 1,3 biphosphoglycerate, adding a phosphate group where the hydrogen was removed.
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What is the 7th enzyme in Glycolysis?
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Phosphoglycerate Kinase
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What does Phosphoglycerate Kinase do?
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Takes 2 ADP, makes 2 ATP.  Removes the 1 phosphate and makes 1,3 biphosphoglycerate into 3-phosphoglycerate.
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What is the 8th enzyme in Glycolysis?
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Phosphoglycerate Mutase
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What does phosphoglycerate Mutase do?
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Re-arranges the phosphate group from 3 to 2 making 3-phosphoglycerate into 2-phosphoglycerate
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What is the 9th enzyme in Glycolysis?
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Enolase
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What does Enolase do?
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Catalyzes a reaction that removes the -OH on C-3 and the -H on C-2 making water.  A double bond forms between C-2 and C-3.  Makes phosphoenolpyruvate.
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What is the 10th enzyme in Glycolysis?
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Pyruvate Kinase
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What does Pyruvate Kinase do?
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Takes 2 ADP and makes 2 ATP.  Changes Phosphoenolpyruvate to Pyruvate
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Substrates

Glucose
Glucose-6-P
Fructose-6-P
Fructose-1,6-diP
Dihydroxyacetone-P
Glyceraldehyde-P
1,3-Biphosphoglycerate
3-Phosphoglycerate
2-Phosphoglycerate (to)
Phosphoenolpyruvate [PEP]
Pyruvate

question
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answer

Enzymes

Hexokinase
Phosphoglucose isomerase
Phosphofructokinase (PFK)
Aldase A
Triose phosphate isomerase
Glyceraldehyde-3-phosphate dehydrogenase
Phosphoglycerate mutase
Enolase
Pyruvate kinase

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