Bacteriology/Mycology – Flashcards

289 test answers

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question
What is the standard incubation temperature for bacteria?
answer
37 C
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At what temperature are plasmids inactivated?
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60 C
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What temperature kills most bacteria?
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80-90 C
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Do Koch's postulates apply to all diseases?
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No
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What is the term for the asymptomatic state of a disease, for example TB?
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Carrier State
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What is necessary to see bacteria properly in light microscopy?
answer
Oil immersion
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Is Staphylococcus aureus Gr - or +?
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Gr +
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In vet med, most cocci are _____.
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Gr +
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What is the shape of S. aureus?
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Cocci in bunches
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What is the shape of Streptococci?
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Cocci in chains
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What is the shape of Pseudomonas?
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Rod
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What is the shape of E. coli?
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Rod
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What is the shape of Campylobacter jejuni?
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Curved rod
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What are 3 examples of branching bacteria?
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Actinomyces, Nocardia, Dermatophilus
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What is the shape of Leptospira?
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Spiral
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What is the shape of Borrelia?
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Spiral
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What is an example of a cocco-bacillary bacteria?
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Pasturella
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What shape is Corynebacterium?
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Pleiomorphic
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What is the shape of Mycoplasma?
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No particular shape
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Give an example of a large, capsulated rod.
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Bacillus anthracis
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What is the #1 bacteria used in biological warfare?
answer
Bacillus anthracis
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What bacteria causes plague?
answer
Yersinia pestis
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Is Francisella tularensis Gr+ or -?
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Gr -
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Is Streptococcus Gr+ or -?
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Gr+
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Is S. aureus penicillin R or S?
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R
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Is Streptococcus penicillin R or S?
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S
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R
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Resistant
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S
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Susceptible
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Is Pseudomonas Gr+ or -?
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Gr -
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Is E. coli Gr+ or -?
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Gr -
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Why can Pseudomonas sometimes be confused with E. coli?
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Both are Gr- rods
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How can Pseudomonas be differentiated from E. coli?
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Pseudomonas is more uniform/E. coli is less uniform
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Is Campylobacter Gr+ or -?
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Gr -
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Is Actinomyces Gr + or -?
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+
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Is Nocardia Gr + or -?
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Gr+
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Is Dermatophilus Gr+ or -?
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Gr+
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What skin disease-causing organism may appear like railroad track?
answer
Dermatophilus
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Is Dermatophilus penicillin R or S?
answer
S
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Is Dermatophilus tetracycline R or S?
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S
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What bacteria causes Lyme disease in humans and dogs?
answer
Borrelia burgdorferi
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Is Lyme disease zoonotic?
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Yes
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What bacteria causes leptospirosis in humans & animals?
answer
Leptospira
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Is Leptospirosis zoonotic?
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Yes
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Is Pasturella Gr + or -?
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Gr -
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What organism causes fowl cholera?
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Pasturella
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Is Corynebacterium Gr + or -?
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Gr+
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What disease does Corynebacterium renale cause in cattle?
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Pyelonephritis
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What Gr + pleiomorphic bacterium resembles Chinese characters?
answer
Corynebacterium
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Is Corynebacterium R or S to penicillin?
answer
100% S
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Is Mycoplasma penicillin R or S?
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R
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Does Mycoplasma have a cell wall?
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No
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What determines whether an organism is Gr + or -?
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Cell wall/Outer envelope
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What is it called when only the ends of the cell stain well?
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Bipolar staining
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What is an example of a bipolar organism?
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Pasturella
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What is the term for bacteria that can not be stained well with Gram stain?
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Acid fast bacteria
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What can be used for bacteria that stain poorly with Gram stain?
answer
Giemsa stain or Silver stain
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What is a sign of dermatophilus infection?
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Superficial skin infection without abscesses
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Give an example of an acid-fast bacteria.
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Mycobacterium
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Can Gr stain be used for Mycobacteria?
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No
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With what bacteria will old cells stain Gr - even though the bacteria is Gr +?
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Clostridium
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What color is the primary stain in acid-fast staining?
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Pink/Red
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What color is the counter stain after decolorization in acid-fast staining?
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Blue
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Can Gr stain be used for Leptospira?
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No
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What stain is used for Leptospira?
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Silver Stain
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How does Leptospira appear when stained with Silver stain?
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Black spirals
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What is an alternative to Gr, Giemsa, & Silver staining?
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Negative Staining (India Ink)
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Give an example of a capsulated bacteria (besides Bacillus).
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Streptococcus pneumoniae, Pasturella
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What bacteria exhibit bipolar staining?
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Pasturella multocida & Yersinia pestis
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What antibiotic can be used to treat Pasturella?
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Tetracycline
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What are some spore-forming bacteria?
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Bacillus; Clostridia
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What are some outer envelope components?
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Capsule, Flagella, Pili/Fimbriae, Outer membrane/LPS, Periplasmic space/Gr-, Cell wall, inner membrane
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What type of bacteria contain LPS?
answer
Gr -
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What type of bacteria have a thick peptidoglycan layer?
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Gr +
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Why do Gr + bacteria retain crystal violet?
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Thick peptidoglycan layer
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What type of bacteria have a thin peptidoglycan layer?
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Gr -
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What type of bacteria have a periplasmic space?
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Gr -
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LPS
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Lipopolysaccharide
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What are some outer envelope structures of E. coli?
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Pili/Fimbriae & Flagella
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How do E. coli attach to mucosa?
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Fimbriae
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What are some bacteria with flagella?
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E. coli, Salmonella
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Does Gram-stain show flagella?
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No
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Bacterial Spores
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Highly R thick-walled oval or spherical bodies
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Is B. anthracis aerobic or anaerobic?
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Aerobic
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What bacteria have terminal spores that are drumstick or tennis racket like in appearance?
answer
Clostridium tetani
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Are Clostridia aerobic or anaerobic?
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Anaerobic
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What is the doubling time of E. coli?
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20min
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What is the doubling time of Mycobacterium tuberculosis?
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24 hr
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Viable, but non-culturable bacteria can be present in the _____ phase.
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Decline
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Many pathogens need ____ for nutrition.
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Blood
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Give an example of a bacteria that requires blood.
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Pasturella
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Does E. coli require blood to grow?
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No
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What are the 4 phases of the bacterial growth curve?
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Lag, Log/Exponential Growth, Stationary, Death/Log Decline
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At what phase of bacterial growth are virulence factors expressed?
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Exponential Growth/Log Phase
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Are most pathogens aerobic, microaerophilic, or anaerobic?
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Aerobic
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Give an example of a microaerophilic bacteria.
answer
Campylobacter jejuni
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Give 2 examples of anaerobic bacteria.
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Fusobacterium, Clostridium
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Give an example of a thermophilic bacterium.
answer
C. jejuni
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What temperature do thermophilic bacteria grow at?
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45 C
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What temperature do psychrophilic bacteria grow at?
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4 C
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Give 2 examples of psychrophilic bacteria.
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Listeria, Yersinia enterocolitica
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What % O2 is normal? What % is microaerophilic?
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20%; 5%
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What is required to cultivate microaerophilic bacteria & anaerobes?
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Special pouches or jars
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What is characteristic of an anaerobic chamber?
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Completely O2-free
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What is another term for freeze drying?
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Lyophilization
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What are 3 methods of preserving bacteria?
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Freeze-drying, freezing at -70C, freezing in liquid Nitrogen (-190C)
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What are 2 methods of bacterial inactivation?
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Thermal/Sterilization, Chemical/Killing by Formalin ~0.5% or beta-propiolactone (vaccines)
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What are 3 preliminary tests for diagnosis of bacterial dz?
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Oxidase, Catalase, Agglutination
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Samples should be collected before _____.
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Antibiotic treatment
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Are dry swabs suitable for sample collection & transport?
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No
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What type of swabs keep specimens moist, leaving bacteria viable, yet not multiplying?
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Transport Swab
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What temperature should samples be held at when transport swabs are used?
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4C
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What is used for sample collection from uterus?
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Guarded Swab
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What type of swab allows aseptic sample collection from the site of infection?
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Guarded Swab
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Direct Gram's smear was taken from a deep wound w/ foul smelling dark discharge. Routine culture on blood & MacConkey agar was (-) for growth. What are the bacteria?
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Anaerobes - Most likely clostridia
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What is used to treat Clostridium?
answer
Penicillin
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What are 2 routinely used culture media?
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Blood & MacConkey Agar
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What bacteria show mucoid colonies on BA & lactose fermenting colonies on Mac agar?
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Klebsiella
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Give an example of a bacteria that can cause otitis externa in dogs, & genital tract infections in horses.
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Klebsiella
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Describe MacConkey agar.
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Selective for Gr -, differential for lactose fermentation
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MA
answer
MacConkey Agar
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What Gr + cocci in bunches show double zone hemolysis on BA?
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S. aureus
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What test is done to differentiate Staphylococcus from Streptococcus?
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Catalase Test
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Describe the Catalase Test. What bacteria will bubble?
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Add a drop of 3% H2O2 to a few colonies on a slide. Staph will bubble.
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Is pseudomonas penicillin S or R?
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R
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What can be used to treat Pseudomonas?
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Gentamycin
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What is the most common cause of wound inf. in N Am?
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Pseudomonas
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Is pseudomonas Oxidase (+) or (-)?
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(+)
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What media will Pseudomonas grow on?
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BA & MA
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What color is a positive oxidase rxn?
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Purple
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What is an example of a commercial bacteria ID sys?
answer
API (Analytical Products Inc) Strip
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For what bacteria is it necessary to use a selective or enriched broth medium to isolate the organism?
answer
Salmonella
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For Salmonella isolation, feces is first inoculated into ______ or ______ & incubated. The ingredients in this media suppress ______ & ____ other than Salmonella. After ___ hr, subculture is done on ___ & ____.
answer
Rappaport or Selenite broth; E. coli & enterics; 18; BA & MA
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Is Salmonella zoonotic?
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Yes
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For isolation & ID of bacteria, always use _____.
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Pure Culture
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After isolation & ID of bacteria, what is done?
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Antibiotic sensitivity testing
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What is one method of antibiotic sensitivity testing?
answer
Disk Diffusion (Kirby-Bauer) Method
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What medium is used for disk diffusion antibiotic sensitivity testing?
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Mueller Hinton agar
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Diagnosis of _____ is done using bacterial agglutination for Ab's.
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Brucellosis
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Latex particle/slide aggln is done for typing of ____
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Streptococci; S. aureus, E coli
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FloAb is used for ____.
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Canine Brucellosis
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ELISA tests are used for antibodies, e.g. _____, or toxins, e.g. _____.
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Lyme dz; Clostridium perfringens
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Serotyping of bacteria is done for ____
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E coli
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DHT is used for ___ & ___.
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Tuberculosis, Glanders
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What are 2 diseases that have a carrier state?
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TB, Brucellosis
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Is TB zoonotic?
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Yes
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What is another name for DTH test?
answer
Mallein Test
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DTH testing is done to diagnose glanders in ____, & TB in ___.
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Horses; Cattle
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What organism causes glanders?
answer
Pseudomonas
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What are 3 bacterial pathogenesis factors?
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Host susceptibility, Bacterial infectivity, Virulence factors
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Give some examples of virulence factors.
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Pili, non-fimbrial adhesins, capsule, toxins
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What organism causes shipping fever in cattle?
answer
Mannheimia haemolytica
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Does Mannheimia haemolytica affect dogs?
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No
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Mannheimia haemolytica is specific to ____.
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Cattle
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E. coli strains that cause diarrhea in neonatal calves do not cause diarrhea in older cattle, b/c _____.
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The older animals have no receptors in the intestine for attachment of E. coli
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E. coli strains which cause Hamburger dz belong to specific _____, & produce _____, which damages ____.
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Serotypes, Shiga toxin, Blood vessels
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What type of toxin is Shiga toxin?
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Enterotoxin
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Capsules help bacteria escape from ____.
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Phagocytosis
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Pathogenic E. coli strains have ____ for attachment to ____, & ability to produce _____.
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Pili, intestinal mucosa, enterotoxin
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Do pathogenic E. coli release enterotoxin first, or bind to intestinal mucosa?
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First bind to mucosa, then release enterotoxin
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An organism that causes dz
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Pathogen
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Establishment of pathogen in host
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Infection
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Give an example of an obligate pathogen.
answer
Bacillus anthracis
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Give an example of a primary pathogen.
answer
Chlamydophila felis
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What organism causes conjunctivitis in cats?
answer
Chlamydophila felis
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Give an example of a secondary pathogen.
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Staph
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Give an example of an opportunistic pathogen
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Staph, E. coli --> UTI
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Give an example of an endogenous inf
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Gingivitis in dogs from commensals
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Give an example of exogenous inf
answer
Bacillus anthracis
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C. tetani produce ____ toxin
answer
Exotoxin
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Give 2 examples of contagious dz
answer
Strangles in horses, Pneumonic plague
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What organism causes pneumonic plague?
answer
Yersinia pestis
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What organism causes Strangles?
answer
Streptococcus equi
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The degree of pathogenicity bacteria may lose or gain
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Virulence
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The process of diminishing virulence
answer
Attenuation
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Is Tetanus an inf?
answer
No - it is an intoxication
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Are clostridium tetani & botulinum contagious?
answer
No
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Give some examples of bacterial entry
answer
Inhalation, Ingestion, Skin/mucosa abrasion, Urogenital tract, Placenta to fetus, Umbilicus
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Give an example of an inhaled bacteria
answer
Bordetella bronchiseptica
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What does Bordetella bronchiseptica cause in dogs? In cats?
answer
Kennel cough, Feline pneumonia
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Give an example of an ingested bacteria.
answer
Salmonella
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Give an example of a bacteria in contaminated water.
answer
Leptospira
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Give an example of a bacteria that enters through the urogenital tract.
answer
Brucella
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Give an example of a bacteria transmitted from placenta to fetus.
answer
Brucella
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Give an example of a bacteria that is transmitted via umbilicus.
answer
E. coli
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Spread by contact, food, water, etc.
answer
Horizontal Transmission
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Transmission from mother to offspring during pregnancy.
answer
Vertical Transmission
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Give an example of a species-specific pathogen.
answer
Streptococcus equi
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Give an example of an organ-specific pathogen.
answer
Brucella abortus/canis (genital organs)
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What structure helps with bacterial attachment and colonization?
answer
Pili
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What factors help bacteria escape the immune system?
answer
Capsules, Toxins
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Name 2 intracellular bacteria that invade host cells.
answer
Mycobacterium, Salmonella
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Name a bacteria with antigenic variation.
answer
Mycoplasma
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Name 2 bacteria that cause apoptosis.
answer
Salmonella, Listeria
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How are virulence factors acquired?
answer
Mutation, Transformation, Transduction, Conjugation
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Bacteriophage enters and codes for virulence factor.
answer
Transduction
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Give an example of a toxin that is transferred via transduction.
answer
Botulism toxin
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What structure, transferred by conjugation, codes for antimicrobial resistance factor & virulence factors?
answer
Plasmid
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Plasmids are transferred by _____.
answer
Conjugation
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Are exotoxins produced by Gr + or - bacteria?
answer
Both
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Examples of exotoxins
answer
Tetanus neurotoxin, Cholera toxin, E coli enterotoxin, Botulism neurotoxin
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Examples of endotoxins
answer
LPS --> Endotoxemia
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Are endotoxins produced by Gr + or - bacteria?
answer
Gr -
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____ are secreted by the bacteria; ____ is a part of the cell wall.
answer
Exotoxins; Endotoxin
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Penicillin ___ bacteria.
answer
Lyses
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What type of toxin is botulism toxin?
answer
Exotoxin
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What toxin causes flaccid paralysis of mm?
answer
Botulism toxin
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What toxin causes spastic contraction of mm & lockjaw?
answer
Tetanus neurotoxin
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What type of toxin is tetanus neurotoxin?
answer
Exotoxin
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Are fluoroquinolones NS or BS?
answer
BS
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What type of drugs should be used to target bacteria?
answer
Specific, NS
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What drug is used to treat S. equi?
answer
Penicillin
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How do penicillins stop infections?
answer
Destroy cell wall
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Give an example of a bactericidal antibiotic.
answer
Penicillin
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Kills bacteria
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Bactericidal
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Inhibits bacterial growth
answer
Bacteriostatic
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Give an example of a bacteriostatic antibiotic.
answer
Tetracycline
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Give 2 examples of NS drug.
answer
Bacitracin, Penicillin G
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Give 2 examples of BS drugs.
answer
Tetracycline, Doxycycline
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What is one chemical grouping of antibiotics?
answer
Beta lactams
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Does bacitracin target Gr + or - bacteria?
answer
Gr -
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What is the aim of antibacterial drugs?
answer
Maintain effective [ ] @ inf site
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Dosage of antibiotics is _____
answer
3-5x MIC
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MIC
answer
Minimal Inhibitory Concentration
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Routes of antibacterial drugs:
answer
PO, IV, IM, SC, Local (eye, ear)
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What drug can be fatal to rabbits, chinchillas, and guinea pigs?
answer
Penicillin & Cephalosporins
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Is Streptococcus equi Penicillin R or S?
answer
S
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Antibacterial drugs should reach _____ @ _____.
answer
Active concentration, inf. site
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What drug is good for urinary, lung, & skin inf?
answer
Baytril/Enrofloxacin
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What drug should not be given PO? Why not?
answer
Penicillin G; destroyed in stomach
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Long-acting drugs such as Liquamycin-LA are administered _____
answer
IM in cattle
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What bacteria causes pink eye in cattle?
answer
Moraxella bovis
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What antibiotics act by inhibition of cell wall synthesis?
answer
Penicillins, Cephalosporins
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What antibiotics act by damage to cell membrane function?
answer
Polymyxins
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What antibiotics act by inhibition of nucleic acid synthesis or function?
answer
Sulfonamides, Quinolones, Enrofloxacin
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What antibiotics act by inhibition of protein synthesis (ribosomes)?
answer
Tetracyclines, Aminoglycosides (Gentamicin)
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What drug is used only in creams (not systemically)
answer
Polymyxins
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What do drug resistant S. aureus produce? What does it do?
answer
Beta-lactamase, destroy B-lactam ring of penicillin, Inactivate drug
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What is a common nosocomial bacterial inf?
answer
S. aureus
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Give examples of Beta lactam drugs.
answer
Penicillins, Cephalosporins, Ampicillin, Amoxicillin, Clavamox, Ceftiofur, Ceftazidime
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Give an example of an Aminoglycoside.
answer
Gentamicin
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Give an example of a Macrolide.
answer
Erythromycin
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Give an example of a Sulfonamide
answer
Trimethoprim
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Give an example of a fluoroquinolone
answer
Enrofloxacin
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Beta lactam drugs are ____.
answer
Bactericidal
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What drug is used to treat Mycoplasma?
answer
Tetracycline
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What drug is used to treat pseudomonas?
answer
Aminoglycosides (Gentamicin), Fluoroquinolones
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What drug is used to treat Campy?
answer
Macrolides
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Is penicillin G NS or BS?
answer
NS
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Is Ampicillin NS or BS?
answer
BS
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What are some potentiated penicillins?
answer
Ampicillin + Sulbactam, Amoxicillin + Clavulanic Acid
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What bacteria is Penicillin G effective against?
answer
Corynebacterium, most Streptococcus
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Is Erysipelothrix penicillin S or R?
answer
S
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Avoid creation of drug-R bacteria by using ____ antibiotics.
answer
NS
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Ampicillin-sulbactam counteracts _______.
answer
Beta lactamase enzyme of bacteria
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Sulbactam inactivates _____, while ampicillin acts on ____.
answer
B-lactamase, bacteria
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B-lactamase enzymes inactivate _____, including ____.
answer
Beta lactam drugs, Amoxicillin
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Which is resistant to B-lactamase? Amoxicillin or Amoxicillin-Clavulanic Acid?
answer
Amoxicillin-Clavulanic Acid
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BS or NS? Clavamox
answer
BS
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Does Clavamox target anaerobes?
answer
Yes
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Is Clavamox active against Pseudomonas?
answer
No
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Is Clavamox active against Mycoplasma?
answer
No
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What is the structure of tetracyclines?
answer
4 Ring structure
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Is enrofloxacin active against anaerobes?
answer
No
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Name 2 antibacterial drugs that are active against anaerobes.
answer
Clindamycin, Metronidazole
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What bacteria do not require susceptibility testing?
answer
Corynebacterium, Erysipelothrix, Bacillus
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Give an example of a synergistic antibiotic.
answer
Sulfa + Trimethoprim
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question
What is one application of sulfa + trimithoprim?
answer
UTI
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question
What is one sporicidal chemical?
answer
Formaldehyde
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What can be used to kill anthrax? For how long?
answer
Formalin 5% spray, 4hrs
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Does Formalin kill spores?
answer
Yes
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question
Natural habitat is soil
answer
Geophilic
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Animals are the source
answer
Zoophilic
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question
Do Malassezia form mycelia?
answer
No
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Ring worm fungi
answer
Dermatomycoses
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Do antibodies protect against fungi?
answer
Rarely
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question
What is a BS antifungal drug?
answer
Ketoconazole ("Nizoral")
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question
What drug is used to treat Candida inf?
answer
Nystatin
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Is nystatin BS or NS?
answer
NS
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What drug is given for ringworm inf? How is it administered?
answer
Griseofulvin, PO
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Is nystatin effective against ringworm?
answer
No
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What is another name for ringworm?
answer
Dermatomycosis
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How is ringworm diagnosed?
answer
Wood's Lamp, KOH Wet mount
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Is ringworm zoonotic?
answer
Yes
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What fungus fluoresces under Wood's lamp?
answer
Ringworm
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How are samples collected for ringworm Dx?
answer
Hair collection, Basal portion/Hair root req'd
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What is the #1 ringworm causing fungus in dogs & cats?
answer
M. canis
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What is the #2 ringworm causing fungus in dogs?
answer
T. mentagrophytes
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Give an example of a systemic anti-fungal drug,
answer
Griseofulvin, Ketoconazole
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question
Give an example of an anti-dermatophyte drug.
answer
Griseofulvin
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