Bacteriology Lab Midterm – Flashcards
Unlock all answers in this set
Unlock answersGive the Gram reaction and morphology [image] |
| Gram-positive cocci (sarcina - tetrads) |
Give the Gram reaction and morphology [image] |
| Gram positive bacilli |
Gram reaction and morphology [image] |
| Gram negative coccobacilli |
Gram reaction and morphology [image] |
| Gram negative bacillus |
Name the type of hemolysis for A [image] |
| beta hemolysis |
| Name the type of hemolysis for B [image] |
| alpha hemolysis |
| Name the type of hemolysis for C [image] |
| gamma hemolysis |
| True or False This catalase test most likely identifies Streptococcus pyogenes [image] |
| False All Streptococcus species are catalse negative |
| What reagent was added to cause this reaction with catalase[image] |
| hydrogen peroxide (H2O2) |
| For this bile solubility test, name the reagent added and the most likely organism we studied. [image] |
| sodium deoxycholate Streptococcus pneumoniae |
| Which number would identify Staphylococcus epidermidis from Staphylococcus aureus in this coagulase test[image] |
The correct answer is #2. Staphylococcus epidermidis is coagulase negative |
#1 is the correct coagulase test for Staphylococcus aureus True or False |
| True Staphylococcus aureus is coagulase positive |
| Which letter is indole negative[image] |
| B |
| Which letter is indole positive[image] |
| A |
| Look at the two MIO tubes labeled #1. The second number always indicates the addition of Kovac's reagent. Interpret tubes #1. [image] |
Indole positive Non-motile |
| Look at the two MIO tubes labeled #2. The second number always indicates the addition of Kovac's reagent. Interpret these tubes. [image] |
| Indole negative Motile Ornithine positive |
Look at the two MIO tubes labeled #3. The second tube indicates the addition of Kovac's reagent. Interpret the results [image] |
Indole positive Motile Ornithine positive |
Which tube is positive for bile esculin? [image] |
| the right tube shows the positive reaction for bile esculin |
| For this oxidase test, which letter would most appropriately match the reaction of E. coli[image] |
B E. coli and most other enterics are oxidase negative |
| Which letter shows an oxidase positive reaction?[image] |
| A |
| Which tube is positive or demonstrates survival in 6.5% NaCl[image] |
| the left tube. Turbidity in salt = positive reaction |
| The tube the arrow points to indicates what hippurate reaction? What Strep organism is associated with this reaction[image] |
| The right tube shows a positive hippurate reaction Streptococcus agalactiae |
| True or False This CAMP test is positive[image] |
| True |
| What two organisms are involved in this reaction[image] |
| Staphylococcus aureus and Streptococcus agalactiae |
| This organism shows what type of reaction for DNase[image] |
| positive |
| On this Mannitol Salt Agar, the most likely organism present is:___________ [image] |
| Staphylococcus aureus |
True or False Staphylococcus saprophyticus demonstrates the following reactivity with Novobiocin [image] |
False Staphylococcus saprophyticus is resistant to Novobiocin, but S. aureus and S. epidermidis are susceptible. |
True or False Streptococcus pyogenes demonstrates the right reaction for PYR [image] |
| 1) True 2) p-dimethylaminoacinnamaldehyde |
| The most likely identity of this organism is: _______ It is Bacitracin ______________ [image] |
Streptococcus pyogenes susceptible |
| The identity of an organism that grows in 6.5% NaCl, Catalase =, Bile Esculin +, and PYR + is: |
| Enterococcus spp. |
| A P disk was placed on this plate. 1) What is the other name for this antibiotic2) What is the most likely organism from Streptococcus[image] |
| 1) Optochin 2) Streptococcus pneumoniae or pneumococcus |
| Interpret the second tube: Red slant/red butt [image] |
No fermentation. Peptone catabolized aerobically and anaerobically with alkaline products. Alk/Alk |
Interpret the third tube: red slant and yellow butt [image] |
Glucose fermentation with acid production. Proteins catabolized aerobically (in the slant) with alkaline products Alk/A |
| Interpret the fourth tube: yellow slant and yellow butt [image] |
| Glucose and lactose and/or sucrose fermentation with acid accumulation in slant and butt. Gas production A/(A) |
| Interpret the fifth tube [image] |
| Glucose and lactose and/or sucrose fermentation with acid accumulation in slant and butt. Gas production and sulfur reduction. A/(A)/+ |
| Interpret the sixth tube [image] |
| Glucose fermentation with acid production. Protein catabolized aerobically (in the slant) with alkaline products. Gas production and sulfur reduction occurred. Alk/(A)/+ |
| Name three organisms with this IMViC reaction: ++-- |
E. coli Edwardsiella tarda Morganella |
| Name three organisms with this IMViC reaction: - - + + |
| Enterobacter aerogenes Enterobacter cloacae Klebsiella pneumoniae |
| True or False This organism requires hematin for growth. The most likely organism is[image] |
| True. This organism requires hematin (aka X factor, hemin) for growth. It's most likely Haemophilus ducreyi |
| What three organisms demonstrate this pattern of growth for X and V factors[image] |
| Haemophilus influenzae, Haemophilus influenzae (aegypticus), and Haemophilus haemolyticus |
| What does this ?-lactamase result indicate[image] |
| The organism produces ?-lactamase making it resistant to ?-lactam antibiotics |
| Interpret these results: [image] |
| The organism is able to do both oxidation and fermentation (or fermentation only). |
| Interpret these results: [image] |
| The organism is able to oxidize sugars |
| Interpret these results: [image] |
| The organism is asaccharolytic or nonsaccharolytic. Doesn't perform sugar metabolism |
| What reagent was added to cause the reaction in the top tube for the phenylalanine deaminase test [image] |
| ferric chloride (FeCl3) |
| What Neisseria species does this[image] |
| Neisseria gonorrhoeae |
What Neisseria species does this? [image] |
| Neisseria lactamica |
What Neisseria species does this? [image] |
| Neiseeria meningitidis |
| Which tube demonstrates citrate positive reaction[image] |
| The tube with the deep dark blue-colored agar |
| What is the name of this phenomenon and what organisms are involved[image] |
satellitism Haemophilus make up the tiny colonies because they require the V factor produced from S. aureus |
Which test tube is positive for methyl-red? [image] |
| Letter B |
| Voges-Proskauer Test Results |
| [image] |
