z-Test #1 – Chemistry Flashcard

Describe Southern Blotting
1. Gel electrophoresis of DNA fragments
2. NaOH denature and blot into nitrocellulose filter
3.Incubate the nitrocellulose bound DNA with 32P labeled DNA or RNA of specific sequences complimentary to DNA
4. Wash unbound probe away
Sucrose
Hetero: aGlucose 1->2 bFructose
Lactose
Hetero: bGalactose 1->4 bGlucose
Cellulose
Homo: bGlucose 1->4 bGlucose
Chitin
Homo: bNAcetylGlucose 1->4 bNAcetylGlucose
Starch
Homo, Branched: aGlucose 1->4 aGlucose
(a-Amylose)
Glycogen
Homo, Branched: aGlucose 1->4 aGlucose 1->6 aGlucose (Amylopectin)
Uronic Acids
sugar in which the terminal carbon’s hydroxyl function has been oxidized to a carboxylic acid
Aldaric Acid
Oxidation of both the terminal hydroxyl group and the aldehyde yields an aldaric acid
Palindromic DNA
For example, the sequence ACCTAGGT is palindromic because its complement is TGGATCCA, which is equal to the original sequence in reverse complement.
Hyaluronic Acid
Gluronic Acid 1->4 NAcetylglucosamine
oligosaccharide
O- or N-linked to compatible amino acid side chains in proteins or to lipid moieties (see glycans).
PCR Temps
Melting: 95
Annealing: 50-65
Polymerization: 72
“Indirect Readout”
A protein senses the base sequence of DNA through the DNA’s backbone conformation and flexibility
Nucleosome
(binds 200bp) (80A/200bp)

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