Unit 6: Protein Synthesis Study Guide KEY – Flashcards

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1. Why is the nucleus called the control center of the cell?
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It manages all of the cell's activities= it "tells" the cell what to do
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2. What are the similarities and differences between DNA and RNA?
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DNA: double stranded, DEOXYRIBOSE sugar, 4 bases (A,T,C,G) RNA: single stranded, RIBOSE sugar, 4 bases (URACIL not T, A, C, G)
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3. What is a gene?
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a section of DNA that codes for a protein that determines a particular trait
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4. What is a genome? .
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the complete set of DNA
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5. What is DNA called the "blueprint of life"?
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It contains instructions for building an organism. It stores the GENETIC CODE for PROTEIN synthesis.
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6. What is the structure of the DNA molecule?
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DOUBLE HELIX of many nucleotides; Backbone contains sugars and phosphates, while the rungs contain the base pairs.
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7. What are the three components of a nucleotide?
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SUGAR, PHOSPHATE, BASE
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8. What type of bond holds the two sides of the DNA ladder together?
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Hydrogen bonds
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9. What two scientists were credited with the discovery of the DNA structure?
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James Watson & Francis Crick
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10. What happens in the process of DNA replication? Where does it occur? .
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Copy of DNA is made in the NUCLEUS: DNA double helix unzips → Copy made from original strand by pairing up the bases → New DNA molecule formed
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11. What are the two purposes for DNA replication?
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Cell Division & Cell Repair
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12. How is DNA replication a semi-conservative process? .
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For each copy of DNA, it is "½ old" & "½ new". Half of the original DNA molecule is saved, or conserved in each new molecule of DNA.
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13. What is the replicated strand of DNA? CGA TGC CAA GTT
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GCT ACG GTT CAA
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14. What is the Central Dogma of Molecular Biology?
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DNA → RNA → protein >the major steps in the transfer of genetic information, from transcription of DNA to RNA to translation of the RNA to protein
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15. What are the TWO stages in protein synthesis and where does each occur?
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1. TRANSCRIPTION → NUCLEUS 2. TRANSLATION → IN CYTOPLASM AT RIBOSOMES
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16. Why does the DNA sequence that codes for a protein need to be copied onto RNA?
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DNA is too big to leave the nucleus. A copy of the gene is made onto mRNA since it is small and can leave the nucleus through the pore.
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17. What are the steps of transcription? Where does it occur? .
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This occurs in the nucleus. ∙DNA partially unzips to expose gene sequence ∙G,U,A,C bases pair up with bases on template DNA ∙mRNA leaves nucleus through pore
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18. What molecule is made during transcription?
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mRNA
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19 (HONORS 3) What are the steps of translation?
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∙Ribosome binds to mRNA at START CODON ∙Codon from mRNA and anticodon from tRNA line up ∙Amino acid gets added to the elongating polypeptide chain ∙Ribosome continues reading until it hits the STOP CODON on the mRNA, then it releases the protein and the mRNA
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20. How do the three kinds of RNA differ? mRNA, rRNA and tRNA
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∙mRNA carries genetic code from DNA to ribosomes ∙rRNA directs translation of mRNA; part of the ribosome ∙tRNA transfers amino acids to the ribosomes where proteins are synthesized THEY ALL MEET AT THE RIBOSOMES TO PARTICIPATE IN PROTEIN SYNTHESIS.
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21. What are the similarities and differences between codons and anticodons? .
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BOTH consist of *3* bases. CODONS are only found on mRNA ONLY 1 ANTICODON is found on a single tRNA; correct amino acid is released when it matches to codon on mRNA
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22. Every codon on a mRNA codes for _____ amino acid.
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Every codon on a mRNA codes for *1* amino acid.
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23. What is a polypeptide chain of amino acids?
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PROTEIN
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24. What type of bond holds the amino acids together in a protein?
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PEPTIDE BONDS
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25a. For the DNA sequence, GAC CTA AGG TGC, that codes for a protein: What is the transcribed mRNA strand? .
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CUG GAU UCC ACG
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25 b. For the DNA sequence, GAC CTA AGG TGC: What is the sequence of anticodons on the tRNA? .
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GAC CUA AGG UGC
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25 c. For the DNA sequence, GAC CTA AGG TGC: How many amino acids will this protein have?
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4 Every CODON codes for 1 Amino Acid. Since there are 4 codons, the protein will have 4 amino acids. Every CODON is made up of 3 bases.
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HONORS 1. During DNA replication, what enzymes play a key role and what do they do?
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HELICASE unwinds helix DNA POLYMERASE adds new nucleotides by matching bases, proofreads new strand DNA LIGASE seals up fragments
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HONORS 2. What are exons and introns?
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EXONS CODE FOR AMINO ACIDS INTRONS DO NOT CONTAIN CODE BOTH found on RNA after transcription occurs. During splicing, introns are removed before mRNA leaves nucleus
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HONORS 4. How does the ribosome know when to start and stop translation?
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During translation, when the ribosome reads the mRNA it begins protein elongation at the INITIATOR (start) CODON. When the ribosome reads the mRNA and it hits the TERMINATOR (Stop) CODON, the protein is complete and translation stops.
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