Unit 1 – Microbiology – Flashcards

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Leeuwenhoek

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'Discovered' the previously unknown microbial world, dubbed the creatures 'beasties.'
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Fungi
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Heterotrophic eukaryotes with cell walls.
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Molds
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Multicellular organisms which sporulate into long intertwined filaments. (Fungi)
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Yeasts
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Unicellular oviods which reproduce asexually through budding. (Fungi)
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Protazoa
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Single cell eukaryotes capable of motion.
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Algae
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Photosynthetic eukaryotes, categorized by their pigmentation and cell walls.
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Unicellular Algae
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Provide most of the world's O2 through photosynthesis, and are a major food source for marine life.
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Parasitic Worms
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Most grow to a visible size, but their microscopic eggs can be found in blood, feces, urine,and lymph specimens.
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Viruses
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Acellular parasites composed of DNA and RNA, not visible under light micrscope.
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Abiogenesis
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Spontaneous generation of life, theory proposed by Aristotle.
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Redi
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Exposed meat to flies, theorized that "animals come from animals."
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Needham
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His experiments confirmed the idea of abiogenesis.
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Spallanzi
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Disproved Needham's experiment.
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Pasteur
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Put the idea of Abiogenesis to rest.
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Pasteur
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His work solidified the scientific method.
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Theories or Laws
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Accepted hypothesises which can explain many observations and be repeated.
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Control Groups
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Necessary for an experiment to be identified as valid.
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Fermentation
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Causes the formation of alcohol to sugar, the formation of lactic acid, and the putrefication of meat.
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Yeast
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Category of microbes responsible for fermentation.
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Biotechnology/Industrial Microbiology
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Filed begun by Pasteur, microbes being intentionally used to manufacture products.
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Buchner
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His experiments demonstrated the existence of enzymes. He also began the study of biochemistry (study of cellular metabolism).
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Germ Theory of Disease
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Pasteur's theory, disease is caused by a specific pathogen. (only applies to;infectious;disease)
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Etiology
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Field founded by Koch, the study of causation of disease.
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Koch
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Discovered disease which causes anthrax, grew multiple bacteria on agar plates.
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Koch
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Took the first photomicrograph of bacteria, as well as the first photograph of bacteria in diseased tissue.
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Koch
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Used sterile lab techniques
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Beijernick
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Pioneered the idea of a filterable virus - non bacterial pathogens which were small enough to go through filters.
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Nosocomial Infections
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Infections which occur in a healthcare setting.
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Semmelweis
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Wanted med students to wash their hands, to rid them of "cadaver particles."
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Lister
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Dressed and sprayed wounds with carbolic acid (phenol).
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Epidemiology
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Study of;occurrence;of distribution and spread of disease in humans, founded by Snow.
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Immunology
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Field founded by Jenner, using vaccines to prevent disease.
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Ehlich
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Sold "magic bullets," after finding that different chemicals could be used to kill microbes differently.
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Biochemistry
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Study of metabolic reactions which occur in all living organisms.
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Molecular Biology
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Study of genome sequencing and evolutionary relationships.
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Woose
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Divided organisms into 3 groups: Bacteria, Archaea, and Eukaryotes.
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Gene Therapy
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The insertion or repair of missing or defective genes.
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Serology
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The study of blood serum.
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  • Growth
  • Reproduction
  • Responsiveness
  • Metabolism
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4 Requirements of Life
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Taxis
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Response to environmental stimuli.
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Organelles
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Specialized structures within a cell.
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Glycocalyx
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Gelatinous;substance surrounding the outside of a cell, composed of polyaccharides or polypeptides. Helps to prevent against;desiccation, and good for attachment of cells. Present in archaea, bacteria, and animal eukaryotic cells.
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Capsule
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Type of glycocalyx, composed of organized repeating units which are firmly attached to cell surface, may prevent bacteria from being recognized by host.
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Slimy Layer
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Type of glycocalyx. Loosely attached, water soluble layers which no not increase the virulence of bacteria.
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Flagella.
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Long structures which extend beyond the cell surface, responsible for motility.
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Filament
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Composed of gobular proteins called flagillin.
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Basal Body
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Anchors the filament and hook to a cell wall by a rod and series of either two or four rings of integral proteins.
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Runs
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Counter clockwise unified movement.;
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Tumbles
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Clockwise independent movement.;
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Positive Taxis
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Movement toward favorable stimuli
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Negative Taxis
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Movement away from negative stimuli
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Fimbrae
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Sticky bristle like projections all over a cell, allow for attachment to other bacteria or cells, ex. biofilms.
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Biofilms
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Slimy masses of microbes adhering to a substrate using fimbrae and glyocalyx.
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Pili
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Allows for the transfer of genetic information between cells.
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  1. N-acetylglucosamine (NAG)
  2. N-acetylmuramic acid (NAM)

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Two glycan proteins which compose the peptidoglyan in bacteria cell walls.
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Gram Positive Bacteria
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  • Have a thick layer of peptidoglycan, which contain techoic acids
  • Negatively Charged
  • Retains purple dye
  • Techoic acids link to lipids, which form lipotechoic acids that anchor the peptidoglycan to cell walls

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Gram Negative Bacteria
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  • Contain a thin layer of peptidoglcan
  • Asymmetric bilayer
  • Lipopolysaccharide outer layer

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lipid a
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The lipid portion of LPS, very toxic and cancause damage if the cell is destroyed
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periplasmic space
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Space between outer and inner membranes, contains periplasam.
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Mycoplasma pneumoniae
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Type of bacteria without a cell wall.
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Mycobacterium
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Contain mycolic acid, which results in the need for an acid-fast stain.
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Cytoplasmic Membrane
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Composed of a phospholipid bilayer, which can be described using the fluid mosaic model. Selectively permeable.
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  • Diffusion
  • Facilitated Diffusion
  • Osmosis

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Name the 3 passive processes.
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Diffusion
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Movement from high concentration to low concentration of small or lipid soluble chemicals.
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Facilitated Diffusion
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Membrane proteins provide pathways for large or electrically charged molecules. Maintain the electrical gradient.
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Permease
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Proteins used in facilitated diffusion, which have a specific binding site for one substance.
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Osmosis
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Diffusion of water across a selectively permeable membrane.
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Isotonic
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Same water concentration across membrane.
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Hypertonic
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Higher concentration of solute vs. water.
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Hypotonic
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Lower concnetration of solute vs. water.
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Uniport
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Transport of one substance.
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Antiport
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Transport of two chemicals in the;opposite;direction.
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Symport
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Two substances moving in the same direction
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Coupled Transport
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The use of one chemical's electrical gradient to transport a second chemical.
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Group Translocation
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Substance being transported is chemically changed.
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Cytosol
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The mostly water, liquid portion of cytoplasm
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Cytoplasm
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Composed of cytosol, inclusions, ribosomes, and the cytoskeleton
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Nucleoid
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The region in prokaryotes which contains the cell's DNA(usually one circular ring), also the site of some chemical reactions.
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Inclusions
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Deposits in cytosol, whichcontain lipids, starch, N, P, or S.Used when nutrients are scarce.
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Endospore
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Vegetative cell transforms into this when under stress.
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Sporulation
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The 8 to 10 hour process of endospore formation, can form centrally, suberminally, or terminally.
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Ribosomes
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Found in cytoplasm, and the site of protein synthesis in bacterial cells.;
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Hami
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Numerous helical filaments on archaea, which 'hook' archaea to surfaces.
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Binary Fission
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1) Cell replicates its DNA

2) Cytoplasmic membrane elongates
3) Wall forms, membrane invanginates

4) Wall completely forms

5) Daughter cells separates

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Snapping Diffsion
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Reproductive process of some gram positive bacilli.
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Spores
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Repoductive cells which can develop into clones of the original.
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Budding
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Out growth of the original cell develops, and is eventually cut off.
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Fragmentation
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Reproductive development of small motile filaments, which leave parental cell.
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Viviparity
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Reproductive process of only Epulospicscum, live offspring emerge from dead mother cell.
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Methonine
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Start codon of archaea.
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Hyperthermophiles
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Require 80C+ to function
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Halophiles
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Live in extremely saline environments.

Need ; 9% NaCl to live

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Bacteriodopsins
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Pigments in halophiles which absorb light energy to pump proteins.
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Methanogens
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Convert CO2 and H2 to CH4
Largest group of Archaea

Responsible for major greenhouse gas

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Deeply Branching Bacteria
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Autotrophs which "branched" off the tree of life very early, live in hot acidic environments.
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Photosynthetic Lamellae
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Pigments located in thylakoids where light is absorbed for photosynthesis.
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Cyanobacteria
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Oxygenic gram negative cocci which are Nitrogen Fixers.
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Oxygenic
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Type of organisms which generate oxygen using chrlorophyl a.
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Nitrogen Fixation
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Reduction of N2 to NH3, ex. cyanobacteria.
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Heterocysts
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Wall in a cell which eparates nitrogen fixation from oxygenic photosynthesis.
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Green and Purple Phototropic Bacteria
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Use bacteriochlorophylls for photosynthesis,andare anoxygenic(don't generate oxygen).
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dehydration
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loss of H+
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Substrate Level Phosphorylation
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Transfer of PO4 3- to ADP from a phosphorylated organic compound.
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oxidative phosphorylation
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Energy from a redox reaction is used to transfer inorganic phosphate to ADP
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Photophosphrylation
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light energy is used to transfer inorganic PO4 3- to ADP
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Yes, ADP is "rechargeable."
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Can ADP be used to generate ATP more than once?
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Enzymes
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Organic Catalysts
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Hydrolases
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Enzyme that adds water in decomposition.
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Isomeraes
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Enzymes which rearrange atoms within a molecule.
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Neither
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Are Isomerases anabolic or catabolic?
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Ligase/Polymerase
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Enzymes which join 2 molecules together.
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Lysases
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Split large molecules without water.
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Oxidoreductases
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Enzymes which oxidize or reduce substrates.
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Transferases
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Enzymes which transfer functional groups between molecules.
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Apoenzymes
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Enzymes which require cofactors to become activated.
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Cofactor
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Non protein ions or coenzymes used to activate enzymes.
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Holoenzyme
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apoenzyme + cofactor =
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Ribozyme
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Enzymes made of RNA,they make protein enzymes.
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Active Site
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The enzyme's functional site.
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Cellular Respiration
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Breakdown of glucose into CO2 and H2O
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