Unit 1 – Microbiology – Flashcards
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Leeuwenhoek |
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'Discovered' the previously unknown microbial world, dubbed the creatures 'beasties.' |
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Fungi |
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Heterotrophic eukaryotes with cell walls. |
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Molds |
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Multicellular organisms which sporulate into long intertwined filaments. (Fungi) |
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Yeasts |
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Unicellular oviods which reproduce asexually through budding. (Fungi) |
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Protazoa |
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Single cell eukaryotes capable of motion. |
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Algae |
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Photosynthetic eukaryotes, categorized by their pigmentation and cell walls. |
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Unicellular Algae |
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Provide most of the world's O2 through photosynthesis, and are a major food source for marine life. |
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Parasitic Worms |
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Most grow to a visible size, but their microscopic eggs can be found in blood, feces, urine,and lymph specimens. |
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Viruses |
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Acellular parasites composed of DNA and RNA, not visible under light micrscope. |
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Abiogenesis |
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Spontaneous generation of life, theory proposed by Aristotle. |
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Redi |
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Exposed meat to flies, theorized that "animals come from animals." |
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Needham |
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His experiments confirmed the idea of abiogenesis. |
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Spallanzi |
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Disproved Needham's experiment. |
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Pasteur |
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Put the idea of Abiogenesis to rest. |
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Pasteur |
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His work solidified the scientific method. |
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Theories or Laws |
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Accepted hypothesises which can explain many observations and be repeated. |
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Control Groups |
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Necessary for an experiment to be identified as valid. |
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Fermentation |
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Causes the formation of alcohol to sugar, the formation of lactic acid, and the putrefication of meat. |
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Yeast |
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Category of microbes responsible for fermentation. |
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Biotechnology/Industrial Microbiology |
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Filed begun by Pasteur, microbes being intentionally used to manufacture products. |
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Buchner |
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His experiments demonstrated the existence of enzymes. He also began the study of biochemistry (study of cellular metabolism). |
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Germ Theory of Disease |
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Pasteur's theory, disease is caused by a specific pathogen. (only applies to;infectious;disease) |
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Etiology |
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Field founded by Koch, the study of causation of disease. |
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Koch |
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Discovered disease which causes anthrax, grew multiple bacteria on agar plates. |
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Koch |
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Took the first photomicrograph of bacteria, as well as the first photograph of bacteria in diseased tissue. |
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Koch |
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Used sterile lab techniques |
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Beijernick |
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Pioneered the idea of a filterable virus - non bacterial pathogens which were small enough to go through filters. |
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Nosocomial Infections |
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Infections which occur in a healthcare setting. |
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Semmelweis |
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Wanted med students to wash their hands, to rid them of "cadaver particles." |
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Lister |
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Dressed and sprayed wounds with carbolic acid (phenol). |
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Epidemiology |
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Study of;occurrence;of distribution and spread of disease in humans, founded by Snow. |
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Immunology |
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Field founded by Jenner, using vaccines to prevent disease. |
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Ehlich |
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Sold "magic bullets," after finding that different chemicals could be used to kill microbes differently. |
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Biochemistry |
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Study of metabolic reactions which occur in all living organisms. |
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Molecular Biology |
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Study of genome sequencing and evolutionary relationships. |
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Woose |
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Divided organisms into 3 groups: Bacteria, Archaea, and Eukaryotes. |
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Gene Therapy |
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The insertion or repair of missing or defective genes. |
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Serology |
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The study of blood serum. |
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4 Requirements of Life |
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Taxis |
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Response to environmental stimuli. |
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Organelles |
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Specialized structures within a cell. |
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Glycocalyx |
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Gelatinous;substance surrounding the outside of a cell, composed of polyaccharides or polypeptides. Helps to prevent against;desiccation, and good for attachment of cells. Present in archaea, bacteria, and animal eukaryotic cells. |
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Capsule |
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Type of glycocalyx, composed of organized repeating units which are firmly attached to cell surface, may prevent bacteria from being recognized by host. |
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Slimy Layer |
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Type of glycocalyx. Loosely attached, water soluble layers which no not increase the virulence of bacteria. |
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Flagella. |
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Long structures which extend beyond the cell surface, responsible for motility. |
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Filament |
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Composed of gobular proteins called flagillin. |
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Basal Body |
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Anchors the filament and hook to a cell wall by a rod and series of either two or four rings of integral proteins. |
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Runs |
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Counter clockwise unified movement.; |
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Tumbles |
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Clockwise independent movement.; |
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Positive Taxis |
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Movement toward favorable stimuli |
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Negative Taxis |
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Movement away from negative stimuli |
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Fimbrae |
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Sticky bristle like projections all over a cell, allow for attachment to other bacteria or cells, ex. biofilms. |
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Biofilms |
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Slimy masses of microbes adhering to a substrate using fimbrae and glyocalyx. |
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Pili |
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Allows for the transfer of genetic information between cells. |
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Two glycan proteins which compose the peptidoglyan in bacteria cell walls. |
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Gram Positive Bacteria |
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Gram Negative Bacteria |
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lipid a |
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The lipid portion of LPS, very toxic and cancause damage if the cell is destroyed |
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periplasmic space |
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Space between outer and inner membranes, contains periplasam. |
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Mycoplasma pneumoniae |
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Type of bacteria without a cell wall. |
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Mycobacterium |
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Contain mycolic acid, which results in the need for an acid-fast stain. |
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Cytoplasmic Membrane |
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Composed of a phospholipid bilayer, which can be described using the fluid mosaic model. Selectively permeable. |
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Name the 3 passive processes. |
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Diffusion |
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Movement from high concentration to low concentration of small or lipid soluble chemicals. |
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Facilitated Diffusion |
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Membrane proteins provide pathways for large or electrically charged molecules. Maintain the electrical gradient. |
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Permease |
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Proteins used in facilitated diffusion, which have a specific binding site for one substance. |
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Osmosis |
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Diffusion of water across a selectively permeable membrane. |
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Isotonic |
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Same water concentration across membrane. |
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Hypertonic |
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Higher concentration of solute vs. water. |
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Hypotonic |
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Lower concnetration of solute vs. water. |
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Uniport |
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Transport of one substance. |
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Antiport |
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Transport of two chemicals in the;opposite;direction. |
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Symport |
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Two substances moving in the same direction |
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Coupled Transport |
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The use of one chemical's electrical gradient to transport a second chemical. |
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Group Translocation |
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Substance being transported is chemically changed. |
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Cytosol |
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The mostly water, liquid portion of cytoplasm |
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Cytoplasm |
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Composed of cytosol, inclusions, ribosomes, and the cytoskeleton |
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Nucleoid |
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The region in prokaryotes which contains the cell's DNA(usually one circular ring), also the site of some chemical reactions. |
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Inclusions |
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Deposits in cytosol, whichcontain lipids, starch, N, P, or S.Used when nutrients are scarce. |
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Endospore |
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Vegetative cell transforms into this when under stress. |
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Sporulation |
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The 8 to 10 hour process of endospore formation, can form centrally, suberminally, or terminally. |
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Ribosomes |
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Found in cytoplasm, and the site of protein synthesis in bacterial cells.; |
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Hami |
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Numerous helical filaments on archaea, which 'hook' archaea to surfaces. |
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Binary Fission |
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1) Cell replicates its DNA 2) Cytoplasmic membrane elongates 4) Wall completely forms 5) Daughter cells separates |
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Snapping Diffsion |
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Reproductive process of some gram positive bacilli. |
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Spores |
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Repoductive cells which can develop into clones of the original. |
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Budding |
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Out growth of the original cell develops, and is eventually cut off. |
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Fragmentation |
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Reproductive development of small motile filaments, which leave parental cell. |
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Viviparity |
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Reproductive process of only Epulospicscum, live offspring emerge from dead mother cell. |
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Methonine |
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Start codon of archaea. |
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Hyperthermophiles |
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Require 80C+ to function |
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Halophiles |
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Live in extremely saline environments. Need ; 9% NaCl to live |
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Bacteriodopsins |
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Pigments in halophiles which absorb light energy to pump proteins. |
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Methanogens |
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Convert CO2 and H2 to CH4 Responsible for major greenhouse gas |
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Deeply Branching Bacteria |
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Autotrophs which "branched" off the tree of life very early, live in hot acidic environments. |
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Photosynthetic Lamellae |
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Pigments located in thylakoids where light is absorbed for photosynthesis. |
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Cyanobacteria |
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Oxygenic gram negative cocci which are Nitrogen Fixers. |
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Oxygenic |
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Type of organisms which generate oxygen using chrlorophyl a. |
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Nitrogen Fixation |
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Reduction of N2 to NH3, ex. cyanobacteria. |
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Heterocysts |
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Wall in a cell which eparates nitrogen fixation from oxygenic photosynthesis. |
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Green and Purple Phototropic Bacteria |
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Use bacteriochlorophylls for photosynthesis,andare anoxygenic(don't generate oxygen). |
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dehydration |
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loss of H+ |
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Substrate Level Phosphorylation |
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Transfer of PO4 3- to ADP from a phosphorylated organic compound. |
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oxidative phosphorylation |
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Energy from a redox reaction is used to transfer inorganic phosphate to ADP |
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Photophosphrylation |
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light energy is used to transfer inorganic PO4 3- to ADP |
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Yes, ADP is "rechargeable." |
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Can ADP be used to generate ATP more than once? |
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Enzymes |
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Organic Catalysts |
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Hydrolases |
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Enzyme that adds water in decomposition. |
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Isomeraes |
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Enzymes which rearrange atoms within a molecule. |
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Neither |
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Are Isomerases anabolic or catabolic? |
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Ligase/Polymerase |
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Enzymes which join 2 molecules together. |
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Lysases |
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Split large molecules without water. |
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Oxidoreductases |
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Enzymes which oxidize or reduce substrates. |
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Transferases |
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Enzymes which transfer functional groups between molecules. |
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Apoenzymes |
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Enzymes which require cofactors to become activated. |
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Cofactor |
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Non protein ions or coenzymes used to activate enzymes. |
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Holoenzyme |
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apoenzyme + cofactor = |
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Ribozyme |
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Enzymes made of RNA,they make protein enzymes. |
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Active Site |
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The enzyme's functional site. |
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Cellular Respiration |
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Breakdown of glucose into CO2 and H2O |