study for lab quiz 1 – Flashcards
Unlock all answers in this set
Unlock answersquestion
| what is the commonly used stain? |
answer
| gram stain |
question
| growth media is used to culture what |
answer
| microbes |
question
| what 3 things do microbes need to grow? |
answer
| nutrients, moisture, and other chemicals |
question
| the shape and arrangement of the cells with their staining properties are used for what ? |
answer
| classifying and preliminary identifying clinical isolates |
question
| what are the 3 stain? |
answer
| gram stain, acid-fast stain, and endospore stain |
question
| what are the two main type of media used? |
answer
| artificial culture media and tissue culture media |
question
| what media is made of agar or broth which contain the necessary nutrients to grow a variety of bacteria and fungi? |
answer
| artificial culture media |
question
| nutrient included in artificial media culture are what 4 things? |
answer
| carbohydrates (glucose, lactose), amino acids, growth factors, and buffers |
question
| what is an example of the type of bacteria that will not grow on artificial culture? |
answer
| chlamydiae and rickettsiae |
question
| what is the genus name of chlamydiae? |
answer
| chlamydia causative agent of the STD chlamydia |
question
| what is the genus name for rickettsiae? |
answer
| rickettsia causative agent of Rocky Mountain Spotted Fever |
question
| what media contains living cells/tissue which act like "host" cells in order to grow and study viruses and other obligate intracellular organisms? |
answer
| cell or tissue culture media |
question
| chlamydia and rickettsia bacteria are both __________ _________ that must have host cells to grow |
answer
| intracellular pathogens |
question
| what is a liquid media that becomes turbid as bacteria grow? |
answer
| broths |
question
| _________ refers to the change that occurs in broth as bacteria grow |
answer
| turbidity |
question
| true or false the more bacteria grow the more they increase the cloudiness of the broth, changing it form the original clear to a "dirty" appearance |
answer
| true |
question
| example to turbidity is going to the swimming pool to ________ __________ |
answer
| jordan lake |
question
| what is a texture much like firm gelatin, but made of seaweed? |
answer
| agar |
question
| when agar is poured into a test tube and allowed to solidify so that the agar surface in the tst tube is slanted it is called? |
answer
| agar slant or "slant" |
question
| agar slant or "slant" is placed in storage for how long? |
answer
| 4-6 weeks |
question
| whan agar is poured into a petri dish and allowed to solidify it is called? |
answer
| agar plates |
question
| what produces a relatively large flat surface for bacterial growth and isolation? |
answer
| agar plates |
question
| what are the 3 different type of agar and broth? |
answer
| non-selective, differential, and selective |
question
| the media that will support the growth of many (but not all) bacteria of clinical interest is called what variety of agar |
answer
| non-selective |
question
| sheep's blood agar (SBA) is what variety of agar? |
answer
| non-selective and differential |
question
| trypticase Soy Agar (TSA) is what variety of agar? |
answer
| non-selective |
question
| chocolate agar is what variety of agar? |
answer
| non-selective |
question
| nutrients agar (NA) is what variety of agar? |
answer
| non-selective |
question
| blood agar is made by adding 5% sheep's blood to a complex nutrient ________ _________ _________media |
answer
| trypticase soy agar |
question
| in blood agar the ______ ________ cells remain intact and the medium resembles fresh blood |
answer
| red blood |
question
| blood agar can be differential by means of what? |
answer
| hemolysis |
question
| what are the 3 hemolysis used in blood agar? |
answer
| gamma hemolysis, beta hemolysis, and alpha hemolysis |
question
| what hemolysis indicates no lysis of RBC's |
answer
| gamma hemolysis |
question
| what hemolysis has bacteria grow on blood agar without disturbing the red blood cell- leaving the RBCs intact? |
answer
| gamma hemolysis |
question
| what hemolysis causes complete breakdown of RBC? |
answer
| beta hemolysis |
question
| what hemolysis causes clearing of the "blood" around the colonies? |
answer
| beta hemolysis |
question
| what hemolysis cause partial breakdown of RBC? |
answer
| alpha hemolysis |
question
| what hemolysis has bacteria lyse the cells but do not completely break down the hemolysis, which leaves a greenish hue (methemoglobin) in the medium? |
answer
| alpha hemolysis |
question
| what not only helps to support the growth of the bacteria, but it also can serve to provide clues to the identity of the bacteria growing? |
answer
| SBA sheep blood agarr |
question
| what variety of agar has media in which some metabolic activity of an organism can be detected by inspection of the growth of the organism on the medium? |
answer
| differential media |
question
| differential media is often accomplished by observing changes in the color of ____ indicator |
answer
| ph |
question
| triple sugar iron agar (TSI) is an example of what type of media? |
answer
| differential media |
question
| mannito salt agar (MSA) is an example of what type of media? |
answer
| differential media |
question
| eosin methylene blue agar is an example of what type of media? |
answer
| differential media |
question
| MacConkey agar is an example of what type of media? |
answer
| differential media |
question
| other non-colorimetric indicators include _______ __________ |
answer
| hemolysis patterns |
question
| in the broadest sense, all media are __________ , in that there is no universal medium on which all species of bacteria can grow |
answer
| selective media |
question
| what type of media is restricted to situation where an ingredient is added which allows the growth of a particular organism, while inhibiting to a considerable extent the growth of other organisms which might be found in the same environment |
answer
| selective media |
question
| normal flora verses pathogen is an example of what type of media |
answer
| selective media |
question
| what selective media agar contains bile salts and crystal violet dye, which inhibits Gram-positive organism? |
answer
| MacConkey agar |
question
| what selective media agar contain small quantities of the antimictobial agents vancomycin, colistin, and nystatin, inhibits the common mictobiota of the genital area, while selection for Neisseria? |
answer
| thayer-Martin agar |
question
| what selective media agar contain 7.5% salt, which inhibits many common microorganisms, but allows for the growth of Staphylococcus species? |
answer
| mannitol salt agar |
question
| what is SALT-TOLERANT? |
answer
| staph |
question
| inhibitors in selective media are (1)_________ in low concentration,(2) ______ _______, high (3)______ concentration and other substances such as (4)__________ __________ are often used to inhibit and/or encourage growth |
answer
| (1) dyes (2) bile salts (3)NaCl (4)phenylethyl alcohol |
question
| many but not all selective media are also _________, often allowing for discrimination between differing species of one genus |
answer
| differential |
question
| what only allows for growth of Staphylococci species, and uses pH indicator and the sugar alcohol Mannitol to distinguish between S. aureus and S. epidermidis species |
answer
| MSA |
