study for lab quiz 1 – Flashcards
56 test answers
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| what are the 3 stain? |
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| gram stain, acid-fast stain, and endospore stain |
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| what are the two main type of media used? |
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| artificial culture media and tissue culture media |
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| what media is made of agar or broth which contain the necessary nutrients to grow a variety of bacteria and fungi? |
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| artificial culture media |
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| nutrient included in artificial media culture are what 4 things? |
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| carbohydrates (glucose, lactose), amino acids, growth factors, and buffers |
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| what is an example of the type of bacteria that will not grow on artificial culture? |
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| chlamydiae and rickettsiae |
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| what is the genus name of chlamydiae? |
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| chlamydia causative agent of the STD chlamydia |
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| what is the genus name for rickettsiae? |
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| rickettsia causative agent of Rocky Mountain Spotted Fever |
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| what media contains living cells/tissue which act like "host" cells in order to grow and study viruses and other obligate intracellular organisms? |
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| cell or tissue culture media |
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| chlamydia and rickettsia bacteria are both __________ _________ that must have host cells to grow |
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| intracellular pathogens |
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| what is a liquid media that becomes turbid as bacteria grow? |
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| broths |
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| _________ refers to the change that occurs in broth as bacteria grow |
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| turbidity |
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| true or false the more bacteria grow the more they increase the cloudiness of the broth, changing it form the original clear to a "dirty" appearance |
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| true |
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| example to turbidity is going to the swimming pool to ________ __________ |
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| jordan lake |
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| what is a texture much like firm gelatin, but made of seaweed? |
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| agar |
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| when agar is poured into a test tube and allowed to solidify so that the agar surface in the tst tube is slanted it is called? |
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| agar slant or "slant" |
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| agar slant or "slant" is placed in storage for how long? |
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| 4-6 weeks |
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| whan agar is poured into a petri dish and allowed to solidify it is called? |
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| agar plates |
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| what produces a relatively large flat surface for bacterial growth and isolation? |
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| agar plates |
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| what are the 3 different type of agar and broth? |
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| non-selective, differential, and selective |
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| the media that will support the growth of many (but not all) bacteria of clinical interest is called what variety of agar |
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| non-selective |
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| sheep's blood agar (SBA) is what variety of agar? |
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| non-selective and differential |
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| trypticase Soy Agar (TSA) is what variety of agar? |
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| non-selective |
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| chocolate agar is what variety of agar? |
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| non-selective |
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| nutrients agar (NA) is what variety of agar? |
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| non-selective |
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| blood agar is made by adding 5% sheep's blood to a complex nutrient ________ _________ _________media |
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| trypticase soy agar |
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| in blood agar the ______ ________ cells remain intact and the medium resembles fresh blood |
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| red blood |
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| blood agar can be differential by means of what? |
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| hemolysis |
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| what are the 3 hemolysis used in blood agar? |
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| gamma hemolysis, beta hemolysis, and alpha hemolysis |
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| what hemolysis indicates no lysis of RBC's |
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| gamma hemolysis |
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| what hemolysis has bacteria grow on blood agar without disturbing the red blood cell- leaving the RBCs intact? |
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| gamma hemolysis |
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| what hemolysis causes complete breakdown of RBC? |
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| beta hemolysis |
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| what hemolysis causes clearing of the "blood" around the colonies? |
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| beta hemolysis |
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| what hemolysis cause partial breakdown of RBC? |
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| alpha hemolysis |
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| what hemolysis has bacteria lyse the cells but do not completely break down the hemolysis, which leaves a greenish hue (methemoglobin) in the medium? |
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| alpha hemolysis |
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| what not only helps to support the growth of the bacteria, but it also can serve to provide clues to the identity of the bacteria growing? |
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| SBA sheep blood agarr |
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| what variety of agar has media in which some metabolic activity of an organism can be detected by inspection of the growth of the organism on the medium? |
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| differential media |
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| differential media is often accomplished by observing changes in the color of ____ indicator |
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| ph |
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| triple sugar iron agar (TSI) is an example of what type of media? |
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| differential media |
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| mannito salt agar (MSA) is an example of what type of media? |
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| differential media |
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| eosin methylene blue agar is an example of what type of media? |
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| differential media |
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| MacConkey agar is an example of what type of media? |
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| differential media |
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| other non-colorimetric indicators include _______ __________ |
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| hemolysis patterns |
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| in the broadest sense, all media are __________ , in that there is no universal medium on which all species of bacteria can grow |
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| selective media |
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| what type of media is restricted to situation where an ingredient is added which allows the growth of a particular organism, while inhibiting to a considerable extent the growth of other organisms which might be found in the same environment |
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| selective media |
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| normal flora verses pathogen is an example of what type of media |
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| selective media |
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| what selective media agar contains bile salts and crystal violet dye, which inhibits Gram-positive organism? |
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| MacConkey agar |
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| what selective media agar contain small quantities of the antimictobial agents vancomycin, colistin, and nystatin, inhibits the common mictobiota of the genital area, while selection for Neisseria? |
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| thayer-Martin agar |
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| what selective media agar contain 7.5% salt, which inhibits many common microorganisms, but allows for the growth of Staphylococcus species? |
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| mannitol salt agar |
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| what is SALT-TOLERANT? |
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| staph |
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| inhibitors in selective media are (1)_________ in low concentration,(2) ______ _______, high (3)______ concentration and other substances such as (4)__________ __________ are often used to inhibit and/or encourage growth |
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| (1) dyes (2) bile salts (3)NaCl (4)phenylethyl alcohol |
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| many but not all selective media are also _________, often allowing for discrimination between differing species of one genus |
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| differential |
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| what only allows for growth of Staphylococci species, and uses pH indicator and the sugar alcohol Mannitol to distinguish between S. aureus and S. epidermidis species |
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| MSA |
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