Pure Culture Techniques – Flashcards
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| Bacteria exist in natural environments as MIXED CULTURES? |
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| True! |
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| Who is the father of medical microbiology? |
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| Robert Koch |
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| What does a pure culture contain? |
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| It contains only a single kind of an organism. |
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| What does a mixed culture contain? |
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| More than one kind of organism. |
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| What does a contaminated culture contain? |
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| The desired organism + unwanted organisms |
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| What are 2 procedures for obtaining pure cultures? |
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1. streak plate 2. pour plate |
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| What is the advantage of pour-plate method? |
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| Requires less skill |
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| What is the disadvantage of the pour-plate method? |
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| Requires more media, tubes and plates. |
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| Why would a culture of S. marcescens not be red if inoculated at a temperature higher than 25 C? |
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| Because higher temperatures inhibit the formation of the organism's red pigment. |
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| What kind of gram is S. marcescens? Shape? |
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| genative short rod |
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| What kind of gram is M. luteus? Shape? |
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| Positive coccus. |
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| What is a colony? |
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| Cells that result from one parents cell. |
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| Why is dilution a necessary prt of pure culture preparation? |
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| For getting isolated colonies. |
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| Before inoculating and pouring molten nutrient agar into a plate, why must the agar first be cooled to 50 C? |
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| In order to avoid condensation of moisture on the cover. |
