question

What color are Serratia marcescens colonies on TSA plates:

answer

Red

question

True or False : A filamentous colony is perfectly circular and self - contained?

answer

False

question

When bacteria grows in broth and is equally spread out, it is called:

answer

Diffused

question

You control the amount of light reaching your specimen on the stage by using the :

answer

Condenser

question

True or False : Candida albicans is an Eukaryote

answer

True

question

True or False : Eukaryotes are generally larger organisms than Prokaryotes.

answer

False

question

Staphylococcus aureus cells are arranged in:

answer

Grape - like Clusters

question

Teh total magnification when using a 40x objective and 10x eyepiece on a microscopeis:

answer

400

question

Why do we bleach the bench tops before and after lab?

answer

So that we don't contaminate our samples with previous microbes on the table surface or leave any after lab

question

True or False : Bacillus subtilis is a Gram negative microbe

answer

False

question

The correct way to write the following species is(italics are analogous to underlining):

answer

Escherichia coli

question

When putting your microscope away, what is something you are NOT to do:

answer

Wipe oil off the oil objective lems with a Kimwipe

question

Which of the following is NOT a cluture mdedium we have used in this class:

answer

Clear Gelattin slant (CGS)

question

True of False : We transfer organisms using only sterile techniques.

answer

False

question

True or False : Pure cultures are only one species of microorganisms.

answer

True

question

Cocci means:

answer

Spherical

question

Bacilli means:

answer

Rod Shaped

question

The correct amount of time to wait to transfer bacteria after you have flame - sterilized your loop is:;

answer

A few moments, and when the medium doesn't sizzle when you touch it

question

True or False : We evaporate the liquid on our loop before putting it in the falame to prevent aerosols.

answer

True

question

True or False : The correct way to open test tubes when you are inoculating them is to have your partner do it.

answer

False

question

The objective lens that uses immersin oil is:

answer

100x

question

If your field of vision is too dim when looking in a microscope a way to correct this is to:

answer

Raise the condenser
Open the iris diaphragm
Be sure that the objective is in the place, over the stage

question

True or False : You should move your inoculating loop in a zipzag pattern when inoculating a slant?

answer

True

question

True or False : Colony and cell morphology will allow you to determine species identification

answer

False

question

Plates need to be incubated upside down because:

answer

It prevents moisture from dripping on the cells and distributing them.

question

True or False : Individuals that are immunocompromised are less susceptible to the normal microbes around them.

answer

False

question

The first person to observe microorganisms was:

answer

Leeuwenhoek

question

The type of microscope we use in lab is:

answer

Bright field

question

When using the microscope for measurement at 1000x, one division is approximately equal to:

answer

0.000001 or 1.0 x 10^-6m

question

True or False: When beginning to look at a specimen in the microscope, you should start with the stage all the way down and move it up until the image comes into focus.

answer

False

question

True or False : Escherichia coli is a Gram negative coccus

answer

False

question

In the case of microbes, the term "ubiquitous" means that:

answer

Microbes are found on all types of surfaces and environments in this world

question

When Bacillus subtilis is grown on agar media, it:

answer

is cream-colored with a lighter center

question

This organism can cause the Toxic Shock Syndrome (TSS) in humans by releasing a dangerous exotoxin. We observed it under the microscope. This organism is:

answer

Staphylococcus aereus

question

When looking at the arrangement of microbial cells, the term "strepto" means:

answer

In chains

question

Which is NOT a typical laboratory procedure?

answer

Sterilize bench
Put unused media back onto shelves
Wash hands
Put all disposable/contaminated material in the biohazard bags after use

question

True or False : You should use the coarse objective adjustment knob after fouxing at 40x power.

answer

False

question

True or False : Growth patters in broth can indicate the oxygen requirements for organisms.

answer

True

question

True or False : Turbidity in a TSB broth tube indicates that it is sterile.

answer

False

question

True or False : A medical scientist who studies the disease and transmission of organisms is an epidemiologist.

answer

True

question

True or False : A colony arises from several different kinds of bacterial cells.

answer

False

question

When performing a cell culture transfer, it is important to:

answer

Flame the opening of the culture tube before and after opening it
Flame the loop before the transfer
Use aseptic technique

question

When agar media is used in test tubes, it is referred to as:

answer

Slants

question

True or False: Solid Media are essential for the separation of bacteria from specimens containing a mixture of organisms

answer

True

question

To prevent aerosols from forming:

answer

Hold the loop just outside the flame until all liquid evaporates and then place the loop in the flame.

question

What would be a possible correction if the microscope field is too dim?

answer

Raise the condenser
Open the iris diaphragm
Be sure the objective is in the place, directly over the stage

question

What possible correction would you do if the microscope has particles of dust or lint blocking your view?

answer

Wipe ocular and objective with lens paper

question

What possible correction would you do if the microscope has floating particles in a hazy field?

answer

To little oil, so the objective is not completely submerged, add oil
Bubbles in the oil, add more oil (be sure the high dry lens in not in oil

question

What shape are A. Cocci?

answer

Spherical Shape

question

What shape are B. Bacilli?

answer

Rod Shaped

question

What shape are C. Spirilla?

answer

Spiral Shaped

question

What is the purpose of washing the laboratory bench top with disinfectannt before transferring cultures?

answer

Microorganisms are ubiquitous. We must learn to handle them asecptically, techniques that prevent contamination. Cultures must not be contaminated with organisms from the lab environment, if they are, your experimental results will be erroneous.

question

How could you determine if the culture media given to you was sterile?

answer

question

What are the signs of bacterial growth in broth media?

answer

Uniform clouding of the broth
small puffballs floating in the broth

question

What are the components of Tryptic Soy Agar?

answer

casein
soybean meal
glucose
sodium chloride
Dipotassium phosphate
Agar

question

What are the components of Blood Agar?

answer

mammalian blood (usually sheep or horse), typically at a concentration of 5–10%
tryptone
agar
sodium chloride

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