Microbiology Chapter 5 : Microbial Metabolism – Flashcards

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the enzyme-substrate complex
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Which of the following is temporarily formed during an enzymatic reaction A. a coenzyme
B. the enzyme-substrate complex
C. a proton gradient
D. activation energy
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the production of glyceraldehyde-3-phosphate by fixation of CO2 molecules
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What is the purpose of the Calvin-Benson cycle A. the formation of phosphorylated pentose sugars from glucose-6-phosphate
B. the catabolism of glucose to pyruvic acid using enzymes different from those of glycolysis
C. transfer of an amine group from one amino acid to another
D. the production of glyceraldehyde-3-phosphate by fixation of CO2 molecules
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glycolysis
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In eukaryotic cells, all of the following processes occur in the mitochondrion EXCEPT:
A. the Krebs cycle
B. electron transport
C. glycolysis
D. beta-oxidation
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30
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NADH generated during cellular respiration results in the production of how many ATP A. 30
B. 4
C. 38
D. 34
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an oxidoreductase
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Which of the following enzymes might be expected to catalyze the removal or addition of electrons of other molecules A. an oxidoreductase
B. a transferase
C. a ligase
D. an isomerase
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All photosynthetic organisms use the same type of chlorophyll
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All of the following are true statements concerning chlorophyll EXCEPT:
A. A magnesium ion is an integral part of a chlorophyll molecule.
B. Chlorophyll molecules function by absorbing light of various wavelengths.
C. All photosynthetic organisms use the same type of chlorophyll.
D. The active site of a chlorophyll molecule is structurally similar to a cytochrome molecule
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fats
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All of the following could be considered precursor metabolites EXCEPT ____________________.
A. glyceraldehyde-3-phosphate.
B. acetyl-CoA.
C. fats.
D. ribose-5-phosphate.
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pyruvic acid
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All of the following are byproducts of fermentation EXCEPT ____________________.
A. pyruvic acid.
B. ethanol.
C. acetic acid.
D. lactic acid.
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coenzyme
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Many vitamins are important for metabolism because they act as ____________________.
A. apoenzymes.
B. holoenzymes.
C. ribozymes.
D. coenzymes
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Both B and C are correct
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Changes in pH have an adverse effect on the shape of an enzyme because pH affects which levels of protein structure A. Primary structure
B. Secondary structure
C. Tertiary structure
D. Both B and C are correct.
E. A, B, and C are correct.
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The absorption of light energy and its conversion to ATP and NADPH
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Which of the following best describes the function of a photosystem A. The absorption of light energy and its conversion to ATP and NADPH
B. The generation of glucose from carbon dioxide and water
C. The generation of NADH and FADH2 from the oxidation of acetyl-CoA
D. The generation of a proton gradient in order to produce ATP
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The regeneration of NAD+ for glycolysis
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What is the major function of fermentation A. The production of ADP
B. The regeneration of NAD+ for glycolysis
C. The generation of a proton gradient
D. The reduction of glucose
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TCA
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All of the following are electron carrier molecules that are important for cellular redox reactions EXCEPT:
A. FAD
B. NAD+
C. TCA
D. NADP+
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competitive inhibition
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Sulfanilamide is a drug which interferes with bacterial enzymes in which of the following ways A. competitive inhibition
B. noncompetitive inhibition
C. feedback inhibition
D. end-product inhibition
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fatty acids
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Beta-oxidation is a method of catabolizing which of the following molecules A. proteins
B. fatty acids
C. glucose
D. glycerol
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polymerization
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The cellular process of linking chemical building blocks together into macromolecules is called ____________________.
A. Oxidation
B. Catabolism
C. Metabolism
D. Polymerization
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production of NADPH
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All of the following are associated with the process of glycolysis EXCEPT ____________________.
A. production of NADPH.
B. phosphorylation of glucose.
C. substrate-level phosphorylation.
D. production of pyruvic acid
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oxidation reactions
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Dehydrogenation reactions are an example of which of the following A. activation reactions
B. oxidation reactions
C. reduction reactions
D. endergonic reactions
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is the amount of energy it takes to trigger a chemical reaction
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Activation energy ____________________.
A. is the same for all chemical reactions.
B. can be achieved by lowering the temperature of a reaction.
C. is the amount of energy it takes to trigger a chemical reaction.
D. is higher in the presence of an enzyme
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electron transport
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All of the following processes are ways in which a cell can use a proton gradient EXCEPT ____________________.
A. active transport.
B. flagellar motion.
C. electron transport.
D. ATP production
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True
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Enzymes work most effectively at their optimal temperature and pH.
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True
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Enzymatic pathways that are regulated through feedback inhibition have at least one allosteric enzyme.
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False
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Both fermentation and aerobic respiration require oxygen.
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True
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Glycolysis produces ATP through substrate phosphorylation.
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False
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Bacterial heterotrophs always use sugar molecules for sources of energy and carbon.
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True
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The synthesis of sugars by using carbon atoms from CO2 gas is called carbon fixation.
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True
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NADPH produced in noncyclic photosynthesis is used to provide electrons for the fixation of CO2 in the Calvin Benson Cycle.
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True
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Biochemical tests for the identification of bacteria involve testing fermentation products or amino acid catabolism.
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False
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Lipids, proteins and sugars all may serve as substrates of glycolysis.
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True
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Metabolic pathways that function in both anabolism and catabolism are called amphibolic pathways.
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Coenzyme
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In the figure shown, what is the small organic molecule needed to activate the apoenzyme called?

 

[image]

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higher; quicker
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[image]

 

In the graph shown, the ________ the temperature, the _______ the enzymatic activity until the optimal temperature is reached.

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It is always a reversible process.
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[image]

 

Which of the following is NOT correct regarding the process of denaturation, seen in this figure?

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Test tube (d)
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Which of the test tubes in the photo is producing both acid and gas?

 

[image]

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Oxygen
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[image]

 

According to the figure, during aerobic respiration, what is the final electron acceptor?

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acetyl
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All of the following are enzymes except which one
urease

threonine deaminase

peroxidase

acetyl
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They are used up in the reactions they catalyze
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All of the following are true of enzymes except:
Answer

Each enzyme has a characteristic three-dimensional shape.

They have active sites that interact with substrate molecules.

They serve as catalysts.

They are used up in the reactions they catalyze.

They lower the activation energy of a reaction.
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increase local temperatures to increase rates of reactions
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Enzymes are important in living organisms because they:
Answer

bring together reactants or properly orient a molecule for a reaction.

bind irreversibly to substrates and remove them from the cellular pool.

interact with and destroy unwanted cellular substrates.

increase local temperatures to increase rates of reactions.

catalyze the covalent bonding of NAD+ molecules to substrates.
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conversion of pyruvic acid to lactic acid
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All of the following are required for the generation of ATP by chemiosmosis except:
Answer

conversion of pyruvic acid to lactic acid.

formation of a H+ ion.

use of proton flow by ATP synthase.

active transport of protons across a phospholipid membrane,

passage of electrons through electron carrier chains.
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competition with the substrate for binding at the active site
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Competitive inhibition of enzyme action involves:

1) competition for binding at the enzyme allosteric site.

2) alteration of the enzyme's active site.

3) destruction of the enzyme substrate.

4) competition with the substrate for binding at the active site.

4) irreversible binding to the enzyme active site.
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Glycolysis, the Krebs Cycle, and the electron transport chain
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The complete oxidation of glucose typically involves which three stagesAnswer

Substrate phosphorylation, pentose phosphate shunt, and the Calvin Cycle.

Photosynthesis, fermentation, and oxidation.

Catabolism, anabolism, and metabolism.

Chemiosmosis, photophosphorylation, and reduction.

Glycolysis, the Krebs Cycle, and the electron transport chain.
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NAD+
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During glycolysis, electrons & hydrogens from the oxidation of glucose are transferred to:
Answer

FADH2.

Lactic acid.

NADH.

FAD.

NAD+
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substrate level phosphorylation
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ATP produced during the Krebs Cycle is formed via a process called:
Answer

photophosphorylation.

oxidative phosphorylation.

chemiosmosis.

ATP anabolism.

substrate level phosphorylation.
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Electrons are transferred to O2
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All of the following steps are involved in the aerobic electron transport chain. Which step happens lastAnswer

Protons move to the outside of the membrane.

NAD+ is formed.

NADH gives up hydrogen.

Electrons are transferred to O2.

Electrons flow between membrane bound electron carriers.
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occurs at the plasma membrane and not the mitochondria
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Unlike eukaryotes, in prokaryotes chemiosmosis:
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electrons are eventually passed to organic molecules instead of inorganic acceptors.

does not require a membrane.

moves iron instead of protons across the membrane.

occurs at the plasma membrane and not the mitochondria.

does not require ATP synthase.
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NO3-
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In anaerobic respiration, which of the following could serve as the final electron acceptorAnswer

O2

NAD+

NO3-

cAMP
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Pyruvic acid
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Which of the following is not a possible end product of fermentationAnswer

Lactic acid.

Carbon dioxide.

Ethanol.

Pyruvic acid.
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Photoautotroph : CO2
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Which organism is not correctly matched to its energy sourceAnswer

Photoautotroph : CO2

Photoheterotroph : light

Chemoheterotroph : glucose

Chemoautotroph : iron

Anoxygenic autotroph : light
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chemoautotrophs
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Organisms that use carbon dioxide as a carbon source and energy sources such as ammonia or hydrogen sulfide are called:
Answer

photoheterotrophs.

photoautotrophs.

chemoheterotrophs.

chemoautotrophs.

saprophytes.
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Krebs cycle
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Which of the following four stages of glucose oxidation requires molecular oxygen?
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oxidative phosphorylation
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Which of the following four stages of glucose oxidation produces the most ATP?
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It is an alternative way to return electron carriers to their oxidized state.
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Which of the following statements about fermentation is true?
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