Microbial Metabolism Flashcard
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Metabolism |
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all chemical reactions taking place within a cell |
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Physiology |
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the study of life processes |
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Anabolism 2 things |
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energy requiring, produces cell components |
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Catabolism 2 things |
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energy yielding, produces waste products |
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Is the cell an open or closed system? |
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open |
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Microbial nutrition |
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supplying cells with the chemical constituents (nutrients) of monomers |
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What are the 4 nutrients that the cells eat |
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macronutrients, micronutrients, essential nutrients, metabolite |
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what percent of the cell is carbon? |
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50 |
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Heterotroph |
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carbon from organic carbon sources (eating from building blocks) |
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Autotroph |
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carbon from CO2, energy from light or organic molecules |
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What percent of the cell is nitrogen? |
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12 |
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ammonia |
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NH3 |
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nitrate |
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NO3- |
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nitrogen |
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N2 |
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Nitrogen fixation |
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fill in card |
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Where is a large source of oxygen? |
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water |
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molecular oxygen is required for what |
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energetic needs and terminal |
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Obligate aerobes |
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require oxygen |
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Obligate anaerobes |
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poisoned by O2 |
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facultative anaerobes |
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can work either way |
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What are some other macronutrients? |
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phosphorus, sulfur, potassium, magnesium, calcium, sodium, iron |
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Micronutrients |
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are organic molecules required in small amounts |
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Vitamins are what? Think physiology |
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coenzymes! |
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What are 4 vitamins mentioned in lecture? |
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thiamine, biotin, pyridoxine, coalbumin |
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Growth factor requirements: phototroph |
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derives all carbon requirements from the principle carbon source (glucose) |
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Growth factor requirements: auxotroph |
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Requires one or more organic nutrients in addition to the principle carbon source. (can make all required C from media along, need one thing provided) |
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Catalysis and enzymes control what? |
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the rate of the reaction |
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What is activation energy? |
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amount of energy required to bring all molecules in a chemical reaction to the reactive state |
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What does a catalyst do? |
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lowers the activation energy, increases rate of reaction and is unchanged |
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What is an enzyme (general) |
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biological catalyst |
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Exoenzyme |
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gets sent outside the cell to aid in absorption from the outside |
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8 things about enzymes |
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proteins, catalyze a specific rxn, substrate, enzyme-substrate complex, active site, product, endoenzymes, exoenzymes |
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What are the two requirements of catalysis? |
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bind correct substrate, position substrate relative to active site |
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What is the structure of enzymes? |
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precise conformation, prosthetic groups, coenzymes |
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What is a prosthetic group? |
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a larger group attached to an enzyme |
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Is the enzyme chemically changed in a reaction? |
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no |
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What is one way that the cell generates energy? |
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oxidation-reduction |
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what is oxidation? |
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removal of electrons |
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what is reduction? |
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addition of electrons |
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what is a redox reaction? |
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e- donor and e- acceptor |
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in a redox rxn, what e- is the energy source? |
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the e- donor (oxidation) |
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What is dehydrogenation |
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removal of H |
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What are the intermediates in electron transfers? |
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electron carriers |
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What is the primary electron donor? |
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electron carriers |
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What is the terminal electron acceptor? |
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electron carriers |
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What kind of energy change is there with electron carrieres? |
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net |
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What are the three stages of redox rxns? |
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removal of electrons from primary donor, transfer of electrons through one or a series of electron carriers, and addition of electrons to terminal acceptor |
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how is energy stored? |
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as ATP |
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What type of bonds are present in the storage of electrons? |
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high energy phosphate bonds |
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ATP 4 points |
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- prime energy carrier - two high energy P bonds - formed during exergonic rxns - drives endergonic rxns |
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Types of Redox: Fermentation--are electron terminals added to aid the process? |
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No, absence of them |
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Types of Redox: Fermentation - what is oxidation couples to? |
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reduction of substrate derived organic compound |
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Types of Redox: Fermentation, what are the products and what is the net ATP production? |
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fermentation products, 2 |
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Types of Redox: Respiration - what is the process |
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O2, some other oxidant is terminal electron acceptor |
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Types of Redox: Respiration: what is oxidation coupled to? |
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reduction of electron acceptor |
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Types of Redox: Reduction - what does it form and what is the ATP production? |
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CO2 and 36 (proks) 38 (euks) |
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respiration and electron transport systems control the transfer of ____ |
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e- |
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Respiration and electron transport systems: what is it? |
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membrane-associated electron carriers |
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Respiration and electron transport systems: Functions |
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Accept, transfer electrons and conserve energy |
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What generates proton motive force? |
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Respiration and electron transport systems |
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What is the proton motive force? |
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electron transport carriers orientated in membrane |
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What occurs across the membrane of the proton motive force? |
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separation of protons from electrons |
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Where are protons transported in proton motive force? |
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outside of the cell |
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Where are electrons transported in proton motive force? |
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through chain to the cell, where they pass to a final acceptor |
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What does the proton motive force generate? |
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a pH gradient and an electrochemical potential |
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What are the two major energy categories of microorganisms? |
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phototrophs and chemotrophs (chemotrophs break down into chemoorganotrophs and chemolithotrophs) |
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How do phototrophs get energy? |
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photosynthesis |
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How do chemotrophs get energy? |
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O2 (chemical energy) |
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Chemotrophs: chemoorgaanotrophs energy requiremetns |
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organic molecules- carbon, etc |
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Chemotrophs: chemolithotrophs energy requirement |
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need inorganic molecules (aid in creating the biosphere) |
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Carbon: energy/carbon categories - photoautotroph |
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photosynthesis (fixes CO2) |
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Carbon: energy/carbon categories - photoheterotroph |
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light but need preformed organic molecules |
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Carbon: energy/carbon categories - chemoheterotroph (chemoorganoheterotroph |
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premade organic molecules and same molecules for building blocks |
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Carbon: energy/carbon categories - chemoheterotroph (chemoorganoautotroph) |
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Get carbon from Carbon fixation |
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Carbon: energy/carbon categories - chemolithoautotroph |
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*** listen to lecture and fill in |