jwcc micro test 1 – Flashcards
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uses biochemical and immunology tests, genetic analysis and microscopic morphology. |
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identification |
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OBSERVE MACROSCOPIC GROWTH CHARACTERISTICS AND MICROSCOPIC MORPHOLOGY |
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INSPECTION |
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GROWTH OF DISCRETE COLONIES ON AGAR |
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ISOLATION |
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PROVIDES THE IDEAL ENVIRONMENT FOR GROWTH |
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INCUBATION |
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THE SAMPLE/SPECIMEN IS INTRODUCED TO THE APPROPRIATE MEDIA |
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INOCULATION |
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THE ELECTRONS PASS THROUGH A SHIN SECTION OF THE SPECIMEN |
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TRANSMISSION ELECTRON MICROSCOPE |
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VISIBLE LIGHT PASSES THROUGH THE SPECIMEN; USES SEPARATE OBJECT AND OCULAR LENSES |
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COMPOUND LIGHT MICROSCOPE |
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DETAILS BECOME VISIBLE BECAUSE OF DIFFERENCES IN THE REFRACTIVE INDEX OF DIFFERENT PARTS OF THE CELL. |
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PHASE-CONTRAST MICROSCOPE |
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VISIBLE LIGHT IS SCATTERED AFTER STRIKING THE SPECIMEN, AND THE SPECIMEN IS VISIBLE AGAINST A DARKENED BACKGROUND. |
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DARKFIELD MICROSCOPE |
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A SPECIAL MICROSCOPE USING ULTRAVIOLET ILLUMINATION. |
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FLUORESCENCE MICROSCOPE |
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THE ELECTRONS STRIKE THE SURFACE OF THE SPECIMEN AND SECONDARY ELECTRONS LEAVING THE SURFACE ARE VIEWED ON A TV SCREEN, PROVIDES 3-D SURFACE IMAGE OF OBJECTS. |
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SCANNING ELECTRON MICROSCOPE |
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MSA |
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DIFFERENTIAL AND SELECTIVE |
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CHOCOLATE AGAR AND THAYER-MARTIN MEDIUM |
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ENRICHED AND SELECTIVE |
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EMB AGAR |
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SELECTIVE AND DIFFERENTIAL |
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SABAROUND'S AGAR |
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SELECTIVE MEDIA |
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TRYPTICASE SOY BROTH/AGAR |
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GENERAL PURPOSE |
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STUART'S AND AMIE'S MEDIA |
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TRANSPORT MEDIA |
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SHEEP BLOOD AGAR |
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DIFFERENTIAL AND ENRICHED |
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BHI (BRAIN HEART INFUSION BROTH) |
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DIFFERENTIAL AND ENRICHED |
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ADHERES BEST TO BACTERIA, WHICH HAVE A NEGATIVE CHARGE, BECAUSE THE COLOR MOLECULE HAS A POSITIVE CHARGE |
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BASIC DYES (+CHARGE) |
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USED IN DIAGNOSIS OF TUBERCULOSIS |
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ACID-FAST STAIN |
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INVOLVES THE USE OF A NEGATIVE STAIN MADE FROM INDIA INK PARTIVLES |
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ACIDIC DYES (-CHARGE) |
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USES MALACHITE GREEN AND APPLICATION OF STEAM |
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ENDOSPORE STAIN |
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REFLECTS THE CHEMICAL DIFFERENCE BETWEEN BACTERIAL CELL WALLS; DIFFERENTIAL STAIN |
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GRAM STAIN |
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CONTAINS ORGANIC SUBSTANCES AND COMPLEX GROWTH FACTORS, SUCH AS BLOOD |
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ENRICHED MEDIA |
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DESIGNED TO SUPPRESS THE GROWTH OF UNWANTED BACTERIA AND TO ENCOURAGE GROWTH OF DESIRED MICROBES |
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SELECTIVE MEDIA |
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INGREDIENTS SUCH AS SODIUM THIOGLYCOLATE CHEMICALLY COMBINED WITH DISSOLVED OXYGEN SUPPORTS GROWTH OF ANAEROBIC BACTERIA |
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GENERAL PURPOSE MEDIA |
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NUTRIENTS ARE DIGESTS OR EXTRACTS; EXACT CHEMICAL COMPOSITION VARIES LITTLE FROM BATCH TO BATCH |
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COMPLEX (NONSYNTHETIC) MEDIA |
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GROWS MOST NONFASTIDIOUS BACTERIA |
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GENERAL PURPOSE MEDIA |
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THE GROWTH OF SEVERAL BACTERIAL TYPES ARE READILY DISTINGUSHED FROM EACH OTHER BASED ON COLOR CHANGES IN THE MEDIA, GROWTH OF DIFFERENT COLORED COLONIES. ECT. |
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DIFFERENTIAL MEDIA |
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IN 1668, DEMONSTRATED THAT MAGGOTS APPEARED ONLY IN DECAYING MEAT THAT HAD BEEN EXPOSED TO FLIES |
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FRANCESCO REDI |
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INTRODUCED THE CONCEPT THAT LIVING CELLS ARISE FROM OTHER LIVING CELLS, BIOGENESIS RABIES VACCINE |
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LOUIS PASTUER |
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VIEWED AND SKETCHED ANIMALCULES WITH HIS SIMPLE MICROSCOPE |
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ANTON VAN LEEUWENHOCK |
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A SURGEON WHO USED CARBOLIC ACID TO CONTROL WOUND INFECTIONS. ASEPTIC TECHNIQUE |
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JOSEPH LISTER |
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DEMONSTRATED THAT INFECTIONS IN OBSTETRICAL WARDS COULD BE MINIMIZED BY DISINFECTING THE HANDS OF PHYSICIANS |
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IGNAZ SEMMELWEIZ |
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USING ANTHRAX AS A MODEL, DEMONSTRATED THAT A SPECIFIC MICROORGANISM IS THE CAUSE OF A SPECIFIC DISEASE, POSTULATES FORMED |
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ROBERT KOCH |
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ORIGINATE OUR SYSTEM OF SCIENTIFIC NOMENCLATURE |
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CARLOUS LINNAEUS (CARL VON LINNE) |
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DEMONSTRATED HEATING DECREASED MICROBES BUT DID NOT RENDER COMPLETE STERILITY |
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JOHN TYNDALL |
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DISCOVERED BACTERIAL ENDOSPORES WERE HEAT RESISTANT |
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FERDINARD COHN |
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DEVELOPED A CLASSIFICATION SYSTEM THAT GROUPS ORGANISMS BY THEIR CELLULAR ORGANIZATION |
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CARL WOESE |
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DISCOVERED PENICILLIN GROWING ON CULTURE PLATES INHIBITED THE GROWTH OF BACTERIA |
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ALEXANDER FLEMING |
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WORKED WITH BACTERIAL CULTURE TECHNIQUES, TB AND ANTHRAX MICROBES |
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ROBERT KOCH |
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ALSO CALLED BLUE GREEN ALGAE; MAY FIX NITROGEN FROM AIR. |
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CYANOBACTERIA |
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PHOTOSYNTHETIC EUCARYOTES |
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ALGAE |
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EUCARYOTES CLASSIFIED PRIMARILY BY THEIR MEANS OF LOCOMOTION |
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PROTOZOA |
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SOURCE OF ANTIBIOTIC, INCLUDES MOLDS AND YEASTS |
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FUNGI |
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GENERAL NAME FOR A ROD-SHAPED BACTERIUM |
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BACILLUS |
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GENERAL NAME FOR A SPHERICAL BACTERIUM |
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COCCUS |
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STUDIES PROTECTIVE SUBSTANCES AND CELLS PRODUCED IN RESPONSE TO INFECTION |
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IMMUNOLOGY |
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The branch of botany dealing with algae. |
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PHYCOLOGY |
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THE STUDY OF PARASITES AND PARASITIC ORGANISMS. |
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PARASITOLOGY |
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STUDY OF FUNGI |
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MYCOLOGY |
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STUDY OF VIRUSES |
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VIROLOGY |
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STUDY OF RELATIONSHIPS BETWEEN MICROBES AND THE ENVIRONMENT |
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ECOLOGY |
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STUDY AND CONTROL THE SPREAD OF DISEASES |
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EPIDEMIOLOGY |
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STUDY OF BACTERIA |
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BACTERIOLOGY |
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microscope used to view objects smaller than 0.2um, such as viruses |
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electron microscope |
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microscope used to view heat fixed stained bacterial cells |
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bright field or compound |
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microscope used to view microorganisms that cdan't be heated and or stained by standard methods |
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dark field |
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microscope to view the internal structure of living organisms |
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phase-contrast |
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microscope that scans the surface features of an object |
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scanning electron microscope |