Helminths – Microbiology Answers – Flashcards
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Ascaris lumbricoides ID methods |
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egg in trichrome stain from stool sample |
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Ascaris lumbricoides diagnosis |
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egg is 55-40 uM, if fertilized it has a thick albumin coat, if non fertilized egg has a thin shell, worms are 10-30cm coming out of the anus |
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Ascaris lumbricoides special characteristics |
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most common in the world, looks like earth worm, nematode |
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Enterobius vermicularis ID methods |
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scotch tape prep to see eggs and larvae, worms can be seen in urine |
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Enterobius vermicularis diagnosis |
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egg has flattened side, 55-25 uM, worms are small |
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Enterobius vermicularis special characteristics |
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most common in US, pinworms, fecal/oral transmission, ingest the eggs they turn into larvae and then worms in intestine, females lay eggs outside the anus, nematode |
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Trichuris trichiura ID methods |
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egg in feces by trichrome |
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Trichuris trichiura diagnosis |
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2 hyaline plugs on eggs, 15-25 uM |
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Trichuris trichiura special characteristics |
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whip worm, fecal oral route transmission, diarrhea, rectal prolapsed = heavy with infestation, seen in United States, nematode |
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Ancyclostoma duodenale/Necator americanus ID methods |
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trichrome stain with eggs in stool, serology |
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Ancyclostoma duodenale/Necator americanus diagnosis |
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transparent egg with colored insides |
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Ancyclostoma duodenale/Necator americanus special characteristics |
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hook worms, eggs look identical in both species, fecal cutaneous transmission; infected larvae can enter through skin, suckers attach to intestine, larvae are infective = filariform, non- infective = rhabidopform, nematode |
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Strongyloides sterocoralis ID methods |
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larvae in feces and sputum, direct prep with iodine |
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Strongyloides sterocoralis diagnosis |
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short buccal cavity, transparent worm |
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Strongyloides sterocoralis special characteristics |
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transmitted fecal cutaneous route, complete life cycle in intestine, nematode |
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Trichinella spiralis ID methods |
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tissue biopsy of muscle |
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Trichinella spiralis diagnosis |
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encysted in muscle tissue |
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Trichinella spiralis special characteristics |
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ingesting undercooked pork, achy, can get into heart and cause an MI, nematode |
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Toxocara canes/cati and braziliense/caninum ID methods |
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eggs seen in O&P, serology or tissue biopsy |
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Toxocara canes/cati and braziliense/caninum diagnosis |
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larvae migrate through tissue, nematode |
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Toxocara canes/cati and braziliense/caninum special characteristics |
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cat and dog feces, ingest eggs, visceral larval migrans and cutaneous larval migrans, nematode |
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Wuchereria bancrofti ID methods |
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Blood smears, thick and thin |
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Wuchereria bancrofti diagnosis |
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sheath +, nuclei not in tail |
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Wuchereria bancrofti special characteristics |
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mosquito vector, elephantitis, blocks lymphs = edema, treadlike filarial, nematode, micro-filarial |
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Loa Loa ID methods |
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Blood smears, thick and thin |
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Loa Loa diagnosis |
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sheath +, 200-300 uM, continuous nuclei in tail |
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Loa Loa special characteristics |
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fly bite, eye worm, nematode, micro-filarial |
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Onchocerca volvulus ID methods |
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Blood smears, thick and thin |
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Onchocerca volvulus diagnosis |
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sheath -, no terminal nuclei |
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Onchocerca volvulus special characteristics |
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river blindness, South America, fly cute, nematode, micro-filarial |
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Brugia malayi/timori ID Methods |
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Blood smears, thick and thin |
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Brugia malayi/timori diagnosis |
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sheath +, discontinuous nuclei, 2 nuclei in tail |
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Brugia malayi/timori special characteristics |
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mosquito, nematode, micro-filarial |
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Taenia solium/saginata ID methods |
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proglotids (segments) in stool or ID egg |
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Taenia solium/saginata diagnosis |
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radial striations around the egg, 35-45 uM |
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Taenia solium/saginata special characteristics |
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pork and beef tapeworm, embedded in nucleus, differentiate via uterine branches (T. saginata has more), ingest proglotids, ingest with India ink and count uterine branches, consume human good, 10 m long, Cestode |
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Diphyllobothrium latum ID methods |
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eggs in feces, proglotids in feces |
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Diphyllobothrium latum diagnosis |
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egg has operculum and knob at terminal end, small uterine branches, egg is 70-45uM |
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Diphyllobothrium latum special characteristics |
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undercooked fish, vitamin B12 deficiency, Cestode |
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Hymenolepsis nana/ diminuta ID methods |
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eggs in feces |
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Hymenolepsis nana/ diminuta diagnosis |
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colorless shell, 3 hooklets inside eggs, 50-30uM |
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Hymenolepsis nana/ diminuta special characteristics |
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dwarf, Cestode |
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Echinococcus granulosus ID methods |
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serology, biopsy cysts |
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Echinococcus granulosus diagnosis |
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3 proglotids, larvae in tissue |
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Echinococcus granulosus special characteristics |
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hydatid cyst disease, dog feces, if cysts burst can cause anaphylactic shock, not in stool, Cestode |
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Fasciolopsis buski ID methods |
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egg in feces, trichrome, direct or concentrated |
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Fasciolopsis buski diagnosis |
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operculum on egg, 150-90 uM |
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Fasciolopsis buski special characteristics |
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fresh water plants contain larvae, intestinal biopsy for worm, Trematode |
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Clonorchis sinensis ID methods |
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egg in feces |
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Clonorchis sinensis diagnosis |
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small egg, operculum and knob, looks like a lemon |
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Clonorchis sinensis special characteristics |
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Chinese liver fluke from undercooked fish, snail vector, liver biopsy, Trematode |
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Fasciola hepatica ID methods |
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egg in feces |
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Fasciola hepatica diagnosis |
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operculum without knob |
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Fasciola hepatica special characteristics |
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liver fluke, water plants, liver biopsy, Trematode |
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Paragonimus westermani ID methods |
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egg in stool |
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Paragonimus westermani diagnosis |
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operculum, 85-55uM, thick posterior |
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Paragonimus westermani special characteristics |
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fresh water crabs, lung fluke, tropica climate, Trematode |
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Schistosoma mansoni/japonicum/haematobium ID methods |
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egg in stool |
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Schistosoma mansoni/japonicum/haematobium diagnosis |
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travel in pairs, S. mansoni egg has lateral spine, s. haematobium egg has terminal spine and s. japonicum has no spine, has knob and tiny lateral spine, Trematode |
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Schistosoma mansoni/japonicum/haematobium special characteristics |
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infected by cercaria invading skin, fecal cutaneous route, snail, fresh water, s. haematobium egg can be seen in urine |
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anacyclostoma braziliense and caninum worm |
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ascaris lumbricoides egg |
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ascaris lumbricoides worm |
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Clonorchis sinensis egg |
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Clonorchis sinensis fluke |
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Diphyllabothrium latum egg |
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Diphyllabothrium latum worm |
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Echinococcus granulosus Hydatid cysts |
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Echinococcus granulosus worm |
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Enterobuis vernicularis egg |
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enterobuis vernicularis worm |
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Fasciola Hepatica egg |
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Fasciola hepatica fluke |
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Fasciolopsis buski egg |
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fasciolopsis buski fluke |
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hook worm egg |
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Hymenolepsis egg |
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Hymenolepsis worm |
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Loa Loa worm |
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Necator americanis and anacyclostoma duodenale larvae |
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Necator americanis and anacyclostoma duodenale worm |
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oncherca volulus worm |
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Paragonimus westermani egg |
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Paragonimus westermani fluke |
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Schitsoma cercaria |
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Schitsoma egg |
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Schitsoma worm |
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Strongyloides sterocroalis egg |
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strongyloides stercoralis worm |
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T. saginata worm |
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T. solium worm |
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Taenia egg |
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Taenia scolex |
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Toxocara canis and cati egg |
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Trichinella spiralis |
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Trichuris trichiura egg |
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trichuria trichiura worm |
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Wuchereria bancrofti |