Genetics Review Ch 17 – Flashcards
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To create transgenic mice DNA is injected into
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a pronucleus of a zygote
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hat is the purpose of the P element in the creation of transgenic Drosophila?
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Efficiently transfer DNA into Drosophila chromosomes
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The Ti plasmid allows for
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the transfer of the plasmid from Agrobacterium to a plant cell.
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Transgenic organisms can help clarify which gene may be responsible for a particular phenotype since
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individuals with the phenotype frequently carry other mutations as well.
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How can transgenics be used in gene expression studies?
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A reporter gene can be cloned so that it is under the control of tissue specific regulatory regions.
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Although bacteria can be used for the production of proteins useful for therapeutic uses what can be a disadvantage to using a prokaryotic system?
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Proteins may not be post-translationally processed correctly
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What is a possible disadvantage of using genetically modified crops?
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The transgene may make its way into non-transgenic plants.
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Transgenic animals can be limited in terms of their study of the links between diseases and specific genes since
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the transgene must be dominant and some diseases are caused by recessive mutations.
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A unique trait of embryonic stem cells that make them useful for generating knockout animals is that they
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are totipotent.
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Why are conditional knockout animals desirable?
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genes that are essential for development can be deleted in the adult.
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Knockin mice can be created using the Cre-lox system. In this procedure the mutation to be knocked in is
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cloned outside of the two lox sites.
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TALENs are bioengineered molecules that include an endonuclease peptide and a
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DNA binding domain created from motifs of a bacterial protein
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Currently the two main vectors for delivery of therapeutic genes for human gene therapy are
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AAV and retroviruses
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What is one problem associated with using retroviruses as human gene therapy vectors?
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Their random integration into the host chromosome may result in detrimental mutations.
