Exam 2 – Microbiology Test – Flashcards
Unlock all answers in this set
Unlock answersquestion
| Blood agar is a chemically defined media. T or F |
answer
| F |
question
| Question: Biofilms are important because I. they faciltate quorum sensing II. they allow bacteria to form two-dimensional structures III. they might facilitate gene transfer IV. they are involved in the majority of bacterial infections V. they show how bacteria do not like to live in communities |
answer
| I, III and IV |
question
| Cyanide poisoning results in the inhibition of ATP synthesis in human cells. Which of the following would you expect to be a mechanism for cyanide poisoning? |
answer
| Binding of Iron in the cytochrome, which is a component of the electron transport chain |
question
| How many molecules of ATP can be generated from the complete oxidation of glucose to CO2 and H2O? |
answer
| 38 |
question
| You streak out a bacterial strain on two nutrient agar plates. You incubate one plate in an anaerobic incubator, while you place the other plate in a regular incubator (both incubators are set at 37C). After two days, you notice that there is no growth on either plate. Assuming you had correct positive controls on each plate, what could you likely conclude about your bacterial strain? |
answer
| My bacterial strain is a microaerophile. |
question
| What is the fate of pyruvic acid in an organism that uses aerobic respiration? |
answer
| It is oxidized in the Krebs cycle. |
question
| Which of the following is not true about anaerobic respiration? |
answer
| It involves glycolysis only. |
question
| A strictly fermentative bacteria produces energy |
answer
| By glycolysis only |
question
| Enzymes that cut at specific DNA sequences are called restriction enzymes True False |
answer
| True |
question
| 2. DNA polymerase makes new DNA by catalyzing which reaction Dehydration of amino acids Condensation of 3 prime hydroxyl and following phosphate group Hydrolysis of the phosphate/oxygen bond Hydrolysis of two phosphate groups |
answer
| Condensation of 3 prime hydroxyl and following phosphate group |
question
| Using your genetic code chart (on page 219), what is the translation of a protein that has the following sequence: AUG GCG UAU UUU CCA GAC CAG UGA UGC CGU AUA UAA Met - Ala - Ile - Lys - Gly - Leu - Val Met - Ala - Tyr - Phe - Pro - Asp - Gln Met - Ala - Tyr - Phe - Pro - Asp - Gln - Gly - Cys - Arg - Ile Met - Arg - Ile - Lys - Gly - Asp - Gln - Gly - Cys - Arg - Ile Met - Arg - Ile - Lys - Pro - Asp - Gln |
answer
| Met - Ala - Tyr - Phe - Pro - Asp - Gln |
question
| Which of the following types of RNA molecules are involved in translation mRNA rRNA tRNA All of these |
answer
| all of these |
question
| 5. Which of the following is true about transformationI. It is the same as conjugation II. It's the process by which "naked" DNA in solution from one bacterium is transferred to another bacterium III. It was observed by Frederick Griffith when he mixed encapsulated S. pneumoniae which had been heat killed with non-encapsulated S. pneumoniae IV. It requires cells that are competent V. It happens naturally among most bacterial genera II, III and IV I, III and V III and V only II and III only II and V only |
answer
| Your Answer: II, III and IV |
question
| The universal genetic code has instructions that specify when to start and stop translating a protein True False |
answer
| True |
question
| 7. The process of making many copies of a DNA molecule is called Gene cloning Western blotting PCR amplification Southern blotting DNA fingerprinting |
answer
| PCR amplification |
question
| You suspect that your patient has a bacterial infection that results in septicemia. You take a blood sample with the idea of looking for bacterial proteins. Unfortunately, you realize that your sample was contaminated with proteases (enzymes that destroy proteins). Which of the following methods could you NOT use for diagnosis Southern blotting Western blotting PCR DNA fingerprinting RFLP |
answer
| western blotting |
question
| The complete collection of an organism’s genes is called its Genome Proteome Chromosome Transcriptome Allele |
answer
| genome |
question
| In addition to RNA polymerase, a helicase is needed for transcription of a gene to begin. True False |
answer
| False |
question
| The method that is used to insert plasmid DNA into bacterial cells is called Transfection Transposition Mutation Transformation Injection |
answer
| Transformation |
question
| 12. (Please refer to the diagram of the Trp operon in your book.) You make a mutation in the Trp repressor protein so that it can no longer bind tryptophan as a corepressor. You expect that the Trp operon would Turn OFF Cease transcription Remain ON Remain OFF Turn ON |
answer
| Remain ON |
question
| Which of the following pairs is mismatched Penicillium - Roquefort cheese Propionobacter - Swiss cheese Acetobacter - vinegar Saccharomyces cerevisiae - Blue Cheese Saccharomyces cerevisiae - beer |
answer
| Saccharomyces cerevisiae - Blue Cheese |
question
| Which of the following is not a DIRECT cause of mutations Chemical mutagens Free radicals Ultraviolet light Faulty DNA replication Rapid cell division |
answer
| Rapid Cell Division |
question
| Mutations can result in which of the following a change in the protein that will negatively affect the organism a change in the protein that will positively affect the organism a change in the protein that is irrelevant to the organism All of these -------------------------------------------------------------------------------- |
answer
| All of these |
question
| Page 1 1. Which of the following species are not inhibited by quaternary ammonium compounds, but instead grow very well on them. Staphylococcus aureus Entamoeba hystolytica Saccharomyces cerevisiae Pseudomonas aeruginosa Herpes simplex virus |
answer
| Pseudomonas aeruginosa |
question
| The first antibiotic discovered was Quinine Salvarsan Streptomycin Penicillin Gentamicin |
answer
| Penicillin |
question
| Which type of reaction does Chloramphenicol inhibit Hydrolysis Transamination Dehydration/Condensation Photosynthesis Carboxylation |
answer
|
question
| 4. Bacterial death will result from damage to which of the following structures? Cell wall Plasma membrane Proteins Nucleic acids All of the above |
answer
| all the above |
question
| Which of the following is best to sterilize heat-labile solutions? Dry heat Membrane filtration Autoclave Pasteurization None of the above |
answer
| Membrane filtration |
question
| Filtration is a physical method of controlling microbial growth True False |
answer
| True |
question
| 7. Which of the following is NOT true about Penicillins Injure the plasma membrane Prevent formation of peptide cross-bridges of peptidoglycan Require that cells are actively dividing to be effective Susceptible to beta-lactamases Can be modified chemically to extend their activity spectrum |
answer
| Injuy plasma membrane |
question
| The E test I. is also called the Kirby-Bauer test II. allows us to estimate the minimal inhibitory concentration for an antibiotic III. results in a zone of inhibition around a circular disk impregnated with the antibiotic IV. uses an increasing gradient of an antibiotic V. is a diffusion method I and III only II, IV and V II only I and IV only II and V only |
answer
| II IV V |
question
| Which of the following methods is NOT used to test antibiotic effectiveness against specific bacteria The Kirby-Bauer test The Disk-Diffusion test The synergism/antagonism diffusion test The gradient diffusion method (E-test) The broth dilution method |
answer
| The synergism/antagonism diffusion test |
question
| Which of the following is NOT a mechanism of drug resistance Destruction or inactivation Change in target site accessibility Alteration of drug target sites Mutation of target site DNA Efflux of drug |
answer
| Mutation of target site DNA |
