Exam 1 Micro Fall 2016 – Flashcards

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What is microbiology? (Definition)
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The study of Microbes
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What are microbes? (definition)
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living organisms too small for the naked eye... microscope
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What are three exceptions to the definition of microbiology?
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Viruses, protozoa/helminthes, and arthropods
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What makes viruses an exception?
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non-living --> no metabolism or homeostasis
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What makes protozoa/helminthes an exception?
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You can see some species without the help of a microscope.
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Why are arthropods considered an exception? What do they include?
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They are big enough to see with naked eye. Include mosquitoes, ticks fleas --> forms of parasites
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How do you organize microbiology?
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Division and fields
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What are the five groups microbes can be organized by types?
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bacteria, viruses, fungi, algae, protozoa
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what is virology?
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study of viruses
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What is bacteriology?
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study of bacteria
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What are pathogens?
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disease causing agents
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What are non-pathogens?
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non disease causing agents
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What is mycology?
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study of fungi
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What is phycology?
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study of algae (micro and macro)
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What is protozoology?
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Study of protozoa
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What is parisitology?
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Study of parasites
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What is a parasite?
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An organism that lives at the expense of a host.
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What is immunology?
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Study of the mechanisms used by one organism to fend off another organism
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What roles do microbes play? (list at least 4)
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industry and environment, decomp, agriculture, bioremediation, biotech, o2 contribution, nutrient recycling
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Antonie van Leeowenhoek
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duch draper, invented first handheld microscope, first to see microbes. Identified the 3 shapes of bacteria
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Edward Jenner
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discovered small pox vaccine
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How did Edward Jenner discover the small pox vaccine?
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Inoculated people with cowpox, something in the cowpox prevented people from attracting small pox
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Louis Pasteur
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Invented Vaccines for rabies and andthrax. Pasteurization. "father of microbiology". came up with germ theory
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Robert Koch
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Came up with Koch's postulate. ID agent of anthrax and TB
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What is Koch's postulate?
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Procedure used to get a pure culture or something.
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Ronald Ross
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ID malaria parasite and found out it was transmitted by mosquitoes
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Alexander Flemming
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INvented penicillin in WWII and found out penicillin can be used to cure syphilis
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Karl Landsteiner
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Human ABO blood groups and Rh factors. First advances in immunology --> Nobel prize winner
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What is taxonomy? (definition)
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Study of phylogenetic relationships b/w organisms
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What was aristotles contribution of microbiology>
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Put all organisms in two groups, mobile and nonmobile
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What is Carolus Linnaeus' nickname?
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Father of taxonomy
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What is Carolus Linnaeus' contribution to microbiology?
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Came up with taxonomy. Kingdom, Phylum, Class, Family, Order, Genus, Species
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Ernest Haeckel
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Added kingdom protista
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What is PH Whittakers contribution to taxonomy?
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expanded to 5 kingdom system: Monera, protista, fungi, plantae, animalia
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What is Carl Woese contribution to taxonomy?
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Able to identify organisms by their sequences of rRNA
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What are the three domains and how are they organized?
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Bacteria achaea and eukarya. organized based on rRNA.
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What falls under eukarya?
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multi-cellular organisms including protista, fungi, plantae, animalia
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How are bacteria and archaea distinguished between each other? What makes them similar?
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rRNA, both prokaryotic unicellular organisms
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What similarities does an archaea cell have to prokaryotes?
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external structures and shape, both lack membranous organelles
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How are archaea cells similar to eukaryotes?
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Have ribosomes and metabolic enzymes
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What is the current record holder for surviving high temps
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Geogamma
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What are extremophiles?
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archaea which require extreme environmental conditions in order to survive: high salt, high temp, acidity
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What are thermophiles?
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archaea which require high temps
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What are halophiles?
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Archaea which require high salt conc.
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What are methanogens
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Archaea which create methane gas, convert organic waste into methane
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How are bacterial cells organized?
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shape size and colony arrangement
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What are the three shapes of bacteria?
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coccus, bacillus, and spirillum
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Describe coccus in one word
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Spherical
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Describe bacillus in one word
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rod
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Describe spirillus in one word
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spiral
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How might a bacterial cell be classified in arrangement? What are the categories?
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Single, pairs (diplo-), Cluster (staphlo-), chain (strepto-), groups of four(tetrads)
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What is the glycocalyx?
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"sweet cup" - outermost surface exposed to environment - sugar layer (glyco)
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What is the functionality of glycocalyx?
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protection, adhesion, and reception
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What are the two forms of glycocalyx?
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Slime layer or capsule
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What is the difference between the two forms of glycocalyx?
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slime layer is a loose shield, can be washed off or removed; capsule is a thick structured layer.
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What is a biofilm?
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aggregates of many bacteria living together or a surface
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What are the functions of the cell wall? (3)
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Determine shape of cell, give structural support so cell can withstand osmotic pressures
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What is the cell wall comprised of?
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mainly peptidoglycan
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Why do drugs and disinfectants target the cell wall?
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when they break down the cell wall it allows the cell to lyse due to osmotic pressures
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What procedure is carried out in order to determine if a cell is gram- or gram+?
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gram staining
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What distinguishing feature does gram+ cell wall have?
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Very thick peptidoglycan layer
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What function does lipoteichoic acid have in gram positive bacteria?
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anchor the cell wall to the cytoplasm membrane
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What function does the teichoic acids have when staining?
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teichoic acids have negative charge which bonds well with crystal violet dye used to determine if gram+
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What distinguishing feature does the gram- cell wall have?
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thin layer of peptidoglycan with additional outer cell membrane composed of lipopolysacharrides, phospholipids, and proteins
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Why are gram- bacteria harder to treat? Hint:lipid A
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The lipid A can shed when given antibiotics, that layer that sheds can trigger toxic substances causing fever, shock, blood clotting
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What is the cell membrane comprised of?
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typical lipid bilayer with proteins embedded in it
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What are endospores?
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unique cell structure separate front he vegetative cell.
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What is the purpose of endospores?
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Survival/defense against hostile or unfavorable conditions
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What are fimbriae?
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fine, hairlike bristles from the cell surface
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What purpose does fimbriae serve?
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Adhesioni to other cells and surfaces
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What is a nucleoid?
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DNA found in a bacterial cell, the condensed area where this is found
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What is a pilus? function?
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Long hollow appendage. Used in transferring DNA to other cells and cell adhesion
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What is a mesosome?
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extension of the cell membrane that folds into the cytoplasm in order to increase surface area
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What is the main function of flagellum?
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motility
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Whatis found in the ribosomes? what purpose do ribosomes serve?
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RNA. site of protein synthesis
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Purpose of Nucleus?
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store DNA
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Purpose of nucleolus?
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Store RNA and Ribosomal subunits
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Purpose of E.R.?
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make proteins and lipids
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Purpose of golgi?
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sorting and protection
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Purpose of Mitochondria?
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power house
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