Beet root cells contains a red pigment, which is found in the cell vacuole. The vacuole has a membrane in which its function is mainly to prevent the betacyanins from leaving the cell. Leakage of these betacyanins into the external solution can be used as an indication of membrance pereambility changes. As part of my experiment, my aim is to see if temperature affects the membrane by witnessing the amount of betacynanins that leaks from the beetroot cells.
The cell membrane covers the outside of a cell and consists of a double layered sheet of lipid molecules interspered with proteins. It seperates the cell from the external environment, gives phyisical protection and allows the import and export of selected chemicals.
Aim:
I will commence the experiment by placing beetroots in a test tube of deionised water, I will use a range of temperatures i
...n which I will place the beetroot cell to see the damage later on. After placing them in series of test tubes of deionised water for 30 minutes. I will pour out each solution into a cuvette and place it in a colorimeter, This will enable me to witness variations in my experiment.
Prediction:
My prediction to this investigation is that, as the tempertaure of the deionised water increases, so will the membrane permeability of the beetroot cell. In doing so, this will cause the beetroot release more betacyanins, because high temperatures have a tendency to damage cell membrane, thus releasing the red pigment (betacyanin).
If the cell membrane and the cell wall are damaged due to high temperature, there will be nothing to prevent the betacyanin from ecsaping the beetroot cell. Hence Membrane permeability would increase.
Variables :
Some of the measuring techniques that I will use during this experiment will be.
- A ruler; This equipment will enable me to measure the beetroot cells accurately and to ensure that the beetroot cells are all the same sizes.
- Digital stopwatch; This will enable me to ensure the beetroot cells stay in the deionised water for the same exact time.
- Measuring cylinder: The amount of deionised water in each test tube has be exactly the same. Otherwise this will create an error in my experiment. Therefore using a measuring cylinder enable my experiment to be fair and accurate.
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