Connect Homework from 23, 21, 24 – Flashcards
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DNA microarrays contain ________.
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single-stranded DNA
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To use a microarray, mRNA from cells of interest are first converted to ________.
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cDNA
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In the microarray procedure, what molecule is labeled with a fluorescent tag?
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cDNA
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True/ False: DNA microarrays are used to study patterns of gene expression.
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True
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True/ False: DNA microarrays may be used to study how a cell responds to specific environmental conditions.
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True
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True/ False: The chromatin immunoprecipitation method can determine whether proteins bind to a particular region of DNA.
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True
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A cell, such as a fertilized egg, that can give rise to all of the cell types in the adult organism.
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Totipotent cell
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A cell that can differentiate into almost every cell type of the body.
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Pluripotent cell
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A cell that can differentiate into several cell types but far fewer than an embryonic stem cell.
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Multipotent cell
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Which of the following are used to introduce cloned genes into cells for human gene therapy? a) liposomes b) retroviral vectors c) T-DNA vectors d) liposomes and retroviral vectors e) retroviral vectors and T-DNA vectors
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liposomes and retroviral vectors
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Zebrafish naturally have black and white stripes. Why are transgenic zebrafish fluorescent?
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The zebrafish shown here are transgenic organisms resulting from a gene addition. These zebrafish received a gene from jellyfish or sea corals that encode a fluorescent protein, causing them to glow green, red, or yellow.
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A _________ is a normal cellular gene that through a ____________ mutation becomes an oncogene.
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proto-oncogene, gain-of-function
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What are the common types of mutations that occur to convert proto-oncogenes to oncogenes?
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- Missense Mutations - Gene Amplification - Chromosomal Translocation
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The proteins ________ work together to cause the release of the transcription factor that is bound by the retinoblastoma protein.
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cyclin and cyclin dependent kinase
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Mutated forms of the Rb protein....
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do not bind to E2F thereby promoting uncontrolled cell division.
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The protein ________ checks for damaged DNA, thereby acting as a "quality control" for the cell.
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p53
