Use of Micropipette & Bacterial Effects of High temperatures – Flashcards
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1000 micropipetor |
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- can only put in a solution up to 1000ul -reads [0][5][5] for 550 |
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| 200 Micropipetor |
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-Only can accrately measure out solutions between 20-180ul -[0][5][5], 55 ul
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| 20 micropipetor |
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-only can measure solutions accurately for 2-18 ul -[0][5][5], 5.5 ul |
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| Hot air oven |
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-instrument used for sterilization |
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| Sterilization |
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| -The process to kill all bacteria in a defined area |
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| autoclave |
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| -sterilization under steam and pressure; endospores will usually be killed within 10-20 minutes |
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| Decimal Reduction Time |
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-reflects the effectiveness of heat killing at a particular temperature -the value of D is the time required for one tenfold reduction in the population |
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Serial Dilution: CFU at time 0 |
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Most accurate # plated x amount of solution [usually 1/10, 10ml]x 10^+power |
