Unit 1 – Microbiology – Flashcards
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            | Leeuwenhoek | 
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        | 'Discovered' the previously unknown microbial world, dubbed the creatures 'beasties.' | 
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            | Fungi | 
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        | Heterotrophic eukaryotes with cell walls. | 
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            | Molds | 
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        | Multicellular organisms which sporulate into long intertwined filaments. (Fungi) | 
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            | Yeasts | 
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        | Unicellular oviods which reproduce asexually through budding. (Fungi) | 
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            | Protazoa | 
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        | Single cell eukaryotes capable of motion. | 
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            | Algae | 
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        | Photosynthetic eukaryotes, categorized by their pigmentation and cell walls. | 
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            | Unicellular Algae | 
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        | Provide most of the world's O2 through photosynthesis, and are a major food source for marine life. | 
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            | Parasitic Worms | 
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        | Most grow to a visible size, but their microscopic eggs can be found in blood, feces, urine,and lymph specimens. | 
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            | Viruses | 
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        | Acellular parasites composed of DNA and RNA, not visible under light micrscope. | 
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            | Abiogenesis | 
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        | Spontaneous generation of life, theory proposed by Aristotle. | 
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            | Redi | 
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        | Exposed meat to flies, theorized that "animals come from animals." | 
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            | Needham | 
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        | His experiments confirmed the idea of abiogenesis. | 
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            | Spallanzi | 
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        | Disproved Needham's experiment. | 
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            | Pasteur | 
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        | Put the idea of Abiogenesis to rest. | 
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            | Pasteur | 
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        | His work solidified the scientific method. | 
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            | Theories or Laws | 
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        | Accepted hypothesises which can explain many observations and be repeated. | 
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            | Control Groups | 
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        | Necessary for an experiment to be identified as valid. | 
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            | Fermentation | 
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        | Causes the formation of alcohol to sugar, the formation of lactic acid, and the putrefication of meat. | 
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            | Yeast | 
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        | Category of microbes responsible for fermentation. | 
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            | Biotechnology/Industrial Microbiology | 
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        | Filed begun by Pasteur, microbes being intentionally used to manufacture products. | 
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            | Buchner | 
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        | His experiments demonstrated the existence of enzymes. He also began the study of biochemistry (study of cellular metabolism). | 
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            | Germ Theory of Disease | 
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        | Pasteur's theory, disease is caused by a specific pathogen. (only applies to;infectious;disease) | 
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            | Etiology | 
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        | Field founded by Koch, the study of causation of disease. | 
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            | Koch | 
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        | Discovered disease which causes anthrax, grew multiple bacteria on agar plates. | 
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            | Koch | 
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        | Took the first photomicrograph of bacteria, as well as the first photograph of bacteria in diseased tissue. | 
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            | Koch | 
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        | Used sterile lab techniques | 
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            | Beijernick | 
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        | Pioneered the idea of a filterable virus - non bacterial pathogens which were small enough to go through filters. | 
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            | Nosocomial Infections | 
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        | Infections which occur in a healthcare setting. | 
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            | Semmelweis | 
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        | Wanted med students to wash their hands, to rid them of "cadaver particles." | 
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            | Lister | 
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        | Dressed and sprayed wounds with carbolic acid (phenol). | 
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            | Epidemiology | 
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        | Study of;occurrence;of distribution and spread of disease in humans, founded by Snow. | 
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            | Immunology | 
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        | Field founded by Jenner, using vaccines to prevent disease. | 
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            | Ehlich | 
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        | Sold "magic bullets," after finding that different chemicals could be used to kill microbes differently. | 
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            | Biochemistry | 
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        | Study of metabolic reactions which occur in all living organisms. | 
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            | Molecular Biology | 
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        | Study of genome sequencing and evolutionary relationships. | 
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            | Woose | 
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        | Divided organisms into 3 groups: Bacteria, Archaea, and Eukaryotes. | 
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            | Gene Therapy | 
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        | The insertion or repair of missing or defective genes. | 
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            | Serology | 
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        | The study of blood serum. | 
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            | 
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        | 4 Requirements of Life | 
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            | Taxis | 
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        | Response to environmental stimuli. | 
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            | Organelles | 
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        | Specialized structures within a cell. | 
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            | Glycocalyx | 
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        | Gelatinous;substance surrounding the outside of a cell, composed of polyaccharides or polypeptides. Helps to prevent against;desiccation, and good for attachment of cells. Present in archaea, bacteria, and animal eukaryotic cells. | 
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            | Capsule | 
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        | Type of glycocalyx, composed of organized repeating units which are firmly attached to cell surface, may prevent bacteria from being recognized by host. | 
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            | Slimy Layer | 
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        | Type of glycocalyx. Loosely attached, water soluble layers which no not increase the virulence of bacteria. | 
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            | Flagella. | 
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        | Long structures which extend beyond the cell surface, responsible for motility. | 
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            | Filament | 
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        | Composed of gobular proteins called flagillin. | 
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            | Basal Body | 
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        | Anchors the filament and hook to a cell wall by a rod and series of either two or four rings of integral proteins. | 
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            | Runs | 
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        | Counter clockwise unified movement.; | 
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            | Tumbles | 
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        | Clockwise independent movement.; | 
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            | Positive Taxis | 
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        | Movement toward favorable stimuli | 
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            | Negative Taxis | 
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        | Movement away from negative stimuli | 
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            | Fimbrae | 
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        | Sticky bristle like projections all over a cell, allow for attachment to other bacteria or cells, ex. biofilms. | 
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            | Biofilms | 
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        | Slimy masses of microbes adhering to a substrate using fimbrae and glyocalyx. | 
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            | Pili | 
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        | Allows for the transfer of genetic information between cells. | 
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            | 
 | 
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        | Two glycan proteins which compose the peptidoglyan in bacteria cell walls. | 
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            | Gram Positive Bacteria | 
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        | 
 | 
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            | Gram Negative Bacteria | 
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        | 
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            | lipid a | 
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        | The lipid portion of LPS, very toxic and cancause damage if the cell is destroyed | 
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            | periplasmic space | 
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        | Space between outer and inner membranes, contains periplasam. | 
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            | Mycoplasma pneumoniae | 
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        | Type of bacteria without a cell wall. | 
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            | Mycobacterium | 
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        | Contain mycolic acid, which results in the need for an acid-fast stain. | 
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            | Cytoplasmic Membrane | 
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        | Composed of a phospholipid bilayer, which can be described using the fluid mosaic model. Selectively permeable. | 
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            | 
 | 
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        | Name the 3 passive processes. | 
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            | Diffusion | 
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        | Movement from high concentration to low concentration of small or lipid soluble chemicals. | 
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            | Facilitated Diffusion | 
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        | Membrane proteins provide pathways for large or electrically charged molecules. Maintain the electrical gradient. | 
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            | Permease | 
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        | Proteins used in facilitated diffusion, which have a specific binding site for one substance. | 
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            | Osmosis | 
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        | Diffusion of water across a selectively permeable membrane. | 
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            | Isotonic | 
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        | Same water concentration across membrane. | 
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            | Hypertonic | 
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        | Higher concentration of solute vs. water. | 
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            | Hypotonic | 
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        | Lower concnetration of solute vs. water. | 
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            | Uniport | 
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        | Transport of one substance. | 
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            | Antiport | 
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        | Transport of two chemicals in the;opposite;direction. | 
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            | Symport | 
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        | Two substances moving in the same direction | 
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            | Coupled Transport | 
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        | The use of one chemical's electrical gradient to transport a second chemical. | 
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            | Group Translocation | 
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        | Substance being transported is chemically changed. | 
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            | Cytosol | 
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        | The mostly water, liquid portion of cytoplasm | 
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            | Cytoplasm | 
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        | Composed of cytosol, inclusions, ribosomes, and the cytoskeleton | 
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            | Nucleoid | 
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        | The region in prokaryotes which contains the cell's DNA(usually one circular ring), also the site of some chemical reactions. | 
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            | Inclusions | 
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        | Deposits in cytosol, whichcontain lipids, starch, N, P, or S.Used when nutrients are scarce. | 
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            | Endospore | 
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        | Vegetative cell transforms into this when under stress. | 
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            | Sporulation | 
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        | The 8 to 10 hour process of endospore formation, can form centrally, suberminally, or terminally. | 
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            | Ribosomes | 
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        | Found in cytoplasm, and the site of protein synthesis in bacterial cells.; | 
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            | Hami | 
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        | Numerous helical filaments on archaea, which 'hook' archaea to surfaces. | 
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            | Binary Fission | 
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        | 1) Cell replicates its DNA 2) Cytoplasmic membrane elongates 4) Wall completely forms 5) Daughter cells separates | 
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            | Snapping Diffsion | 
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        | Reproductive process of some gram positive bacilli. | 
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            | Spores | 
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        | Repoductive cells which can develop into clones of the original. | 
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            | Budding | 
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        | Out growth of the original cell develops, and is eventually cut off. | 
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            | Fragmentation | 
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        | Reproductive development of small motile filaments, which leave parental cell. | 
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            | Viviparity | 
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        | Reproductive process of only Epulospicscum, live offspring emerge from dead mother cell. | 
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            | Methonine | 
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        | Start codon of archaea. | 
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            | Hyperthermophiles | 
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        | Require 80C+ to function | 
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            | Halophiles | 
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        | Live in extremely saline environments. Need ; 9% NaCl to live | 
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            | Bacteriodopsins | 
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        | Pigments in halophiles which absorb light energy to pump proteins. | 
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            | Methanogens | 
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        | Convert CO2 and H2 to CH4 Responsible for major greenhouse gas | 
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            | Deeply Branching Bacteria | 
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        | Autotrophs which "branched" off the tree of life very early, live in hot acidic environments. | 
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            | Photosynthetic Lamellae | 
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        | Pigments located in thylakoids where light is absorbed for photosynthesis. | 
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            | Cyanobacteria | 
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        | Oxygenic gram negative cocci which are Nitrogen Fixers. | 
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            | Oxygenic | 
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        | Type of organisms which generate oxygen using chrlorophyl a. | 
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            | Nitrogen Fixation | 
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        | Reduction of N2 to NH3, ex. cyanobacteria. | 
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            | Heterocysts | 
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        | Wall in a cell which eparates nitrogen fixation from oxygenic photosynthesis. | 
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            | Green and Purple Phototropic Bacteria | 
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        | Use bacteriochlorophylls for photosynthesis,andare anoxygenic(don't generate oxygen). | 
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            | dehydration | 
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        | loss of H+ | 
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            | Substrate Level Phosphorylation | 
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        | Transfer of PO4 3- to ADP from a phosphorylated organic compound. | 
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            | oxidative phosphorylation | 
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        | Energy from a redox reaction is used to transfer inorganic phosphate to ADP | 
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            | Photophosphrylation | 
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        | light energy is used to transfer inorganic PO4 3- to ADP | 
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            | Yes, ADP is "rechargeable." | 
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        | Can ADP be used to generate ATP more than once? | 
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            | Enzymes | 
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        | Organic Catalysts | 
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            | Hydrolases | 
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        | Enzyme that adds water in decomposition. | 
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            | Isomeraes | 
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        | Enzymes which rearrange atoms within a molecule. | 
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            | Neither | 
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        | Are Isomerases anabolic or catabolic? | 
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            | Ligase/Polymerase | 
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        | Enzymes which join 2 molecules together. | 
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            | Lysases | 
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        | Split large molecules without water. | 
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            | Oxidoreductases | 
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        | Enzymes which oxidize or reduce substrates. | 
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            | Transferases | 
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        | Enzymes which transfer functional groups between molecules. | 
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            | Apoenzymes | 
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        | Enzymes which require cofactors to become activated. | 
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            | Cofactor | 
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        | Non protein ions or coenzymes used to activate enzymes. | 
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            | Holoenzyme | 
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        | apoenzyme + cofactor = | 
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            | Ribozyme | 
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        | Enzymes made of RNA,they make protein enzymes. | 
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            | Active Site | 
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        | The enzyme's functional site. | 
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            | Cellular Respiration | 
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        | Breakdown of glucose into CO2 and H2O | 
