Microbiology chapters 4, 5, & 6 – Flashcards

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Eukaryotic Cell Characteristics

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  • animal, plant, fungi, and protists
  • Contain membrane- bound organelles
  • contain nucleus with DNA
  • ribosomes
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Prokaryotic Cell Characteristics
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  • NO nucleus
  • No membrane bound organelle
  • ribosomes
  • cytoplasm
  • chromosomes
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Characteristics of Life
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  • reproduction & heredity
  • growth and development
  • metabolism
  • movement or irritability
  • Cell support
  • Transport
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Flagella
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3 parts:

      filaments

        basal body

hook

 

pro: 360degree movement                 

 

Euk: whip

       9 + 2 arrangement

 

functions: motility

 

 

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Flagellar Arrangements
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Monotrichous- single flagellum at one end

 

Lophotrichous- small bunches ermerging from the same site

 

Amphitrichous- flagella at both ends of the cell

 

Peritrichous- flagella dispersed over surface of cell; slowest

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Flagellar Responses

 

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run-counterclockwise

tumble- clockwise

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Stimulus
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no stimulus- short movements

stimulus- long movements gradient of attractant concentration

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Fimbrae
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  • hairlike projections
  • used for attatchment
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Pili
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  • found only in Gram neg cells
  • join bacteria cells for partial DNA transfer
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Glycocalyx
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2 types: capsule or slime layer

 

coating of the molecule external to the cell wall

 

functions: protection from dehydration

inhibit killing of wbc

attatchment; adherence

signal reception

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Biofilm
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group of bacteria working together collectively

 

 

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Cell Envelope
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external covering outside the cytoplasm

 

contains cell wall and cell membrane

 

gram stain:

        positive- thick layer of peptidoglycan

                     has more cross-linking

 

        negative- thin layer of peptidoglycan

                      has outer membrane

 

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Cell Wall
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functions:

   structure

   protection

 

 

peptidoglycan is main component

 

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Gram Stain
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gram +: retain crystal violet and stain purple

gram -: lose crystal violet and stain red

 

Crystal violet -> stains peptidoglycan

Gram's Iodine -; sets the stain by causing clumping

Alcohol -; dissolves the membran; decolorizes gram -

Saferin -; counterstains gram -

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Cell Membrane
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phospholipid bilayer- selectively permeable

;

functions:

;;;;; provide site for energy reaction, nutreint processing, and synthesis.

;

;

;

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Cytoplasm
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dense gelatinous solution

;

a solvent for materials used in cell functions

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Ribosomes
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site of protein synthesis

;

;

prokaryotic- smaller and less in number

eukaryotic- bigger and more in number

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Inclusions
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intracellular storage bodies

;

;

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Nucleoid
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location of DNA

;

not membrane bound

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Endospores
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inactive metabolic state, which produces a thick glycocalyx during a vegetative state
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Bacterial Shapes
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Coccus- spherical

;

Bacillus- rod

;

Spirillum- spiral

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classification
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Genetic (phylogenic)- classified by composition of DNA

;

Phenogenic- classification by appearance

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Endosymbiotic Relationship
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Organelles originated from prokaryotic cells

;

Organelles: mitochondria and cytoplasm

;

why?

;; has own dna

;; has prokaryotic ribosomes

;; double membrane

;; replicates independently

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Cilia
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hairlike projections on the surface of cell

;

only in animal cells and some protozoa

;

functions:

;;;;; motility

;;;;; feeding

;;;;; filtering

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nucleolus
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inside nucleus

site of ribosome synthesis

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Endoplasmic Reticulum
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RER- has ribosomes on top

;;;;;;; proteins are synthesized

;

;

SER-lacks ribosomes

;;;;; lipids are synthesized

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Golgi Apparatus
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;where Proteins are modified and matured and sorted
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Lysosomes
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digestive enzymes

digesting food molecules and ivading microbes

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mitochondria
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produces atp
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chloroplast
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photosynthesis
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Fungi
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mushrooms, puffballs, gill fungi

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unicellular or colonial

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yeast or hyphae; sometimes both dimorphic

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heterotrophic

;

;

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reproduction

;

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asexual reproduction: spores are formed through budding or mitosis

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Sexual reproduction: spores are formed by fusion of two seperate strains

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Protista
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algae-unicellular and colonial; photosize with chlorophyll

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protozoa-unicellular and lack tissue. heterotrophic

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viruses
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nonliving

;

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capsids
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protect nucleic acid

;

enveloped- with envelope

naked-without envelope

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spikes
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proteins that attatch to host cell and determine which cells to infect
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Matrix Proteins
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essential for replication

not in all

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structures
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Helical

Icosahedral

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animal virus multiplication
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Adsorption- attatchement to host

Penetration- virus enters to host

uncoating- nucleic acid is released through the celll

synthesis- viral components are produced

assembly- new viral particles are constructed

release- assembled virus are released by budding or lysis

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Lysogeny
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Adsorption- attachment to cell

penetration- virus goes into cell

replicaton- virus material is replicated

assembly- assembly of new virus

maturation

cell breaks apart and virus are released

;

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prions
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misfolded proteins that induce normal proteins to misfold
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mitosis
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interphase-resting state

prophase- condensation of chromosomes

Metaphase- chromosomes line up

Anaphase- chromosomes pull cells apart

telophase- nuclear envelope reform, cytokenesis occurs

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