jwcc micro test 1 – Flashcards

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uses biochemical and immunology tests, genetic analysis and microscopic morphology.
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identification
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OBSERVE MACROSCOPIC GROWTH CHARACTERISTICS AND MICROSCOPIC MORPHOLOGY
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INSPECTION
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GROWTH OF DISCRETE COLONIES ON AGAR
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ISOLATION
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PROVIDES THE IDEAL ENVIRONMENT FOR GROWTH
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INCUBATION
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THE SAMPLE/SPECIMEN IS INTRODUCED TO THE APPROPRIATE MEDIA
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INOCULATION
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THE ELECTRONS PASS THROUGH A SHIN SECTION OF THE SPECIMEN
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TRANSMISSION ELECTRON MICROSCOPE
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VISIBLE LIGHT PASSES THROUGH THE SPECIMEN; USES SEPARATE OBJECT AND OCULAR LENSES
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COMPOUND LIGHT MICROSCOPE
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DETAILS BECOME VISIBLE BECAUSE OF DIFFERENCES IN THE REFRACTIVE INDEX OF DIFFERENT PARTS OF THE CELL.
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PHASE-CONTRAST MICROSCOPE
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VISIBLE LIGHT IS SCATTERED AFTER STRIKING THE SPECIMEN, AND THE SPECIMEN IS VISIBLE AGAINST A DARKENED BACKGROUND.
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DARKFIELD MICROSCOPE
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A SPECIAL MICROSCOPE USING ULTRAVIOLET ILLUMINATION.
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FLUORESCENCE MICROSCOPE
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THE ELECTRONS STRIKE THE SURFACE OF THE SPECIMEN AND SECONDARY ELECTRONS LEAVING THE SURFACE ARE VIEWED ON A TV SCREEN, PROVIDES 3-D SURFACE IMAGE OF OBJECTS.
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SCANNING ELECTRON MICROSCOPE
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MSA
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DIFFERENTIAL AND SELECTIVE
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CHOCOLATE AGAR AND THAYER-MARTIN MEDIUM
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ENRICHED AND SELECTIVE
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EMB AGAR
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SELECTIVE AND DIFFERENTIAL
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SABAROUND'S AGAR
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SELECTIVE MEDIA
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TRYPTICASE SOY BROTH/AGAR
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GENERAL PURPOSE
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STUART'S AND AMIE'S MEDIA
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TRANSPORT MEDIA
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SHEEP BLOOD AGAR
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DIFFERENTIAL AND ENRICHED
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BHI (BRAIN HEART INFUSION BROTH)
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DIFFERENTIAL AND ENRICHED
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ADHERES BEST TO BACTERIA, WHICH HAVE A NEGATIVE CHARGE, BECAUSE THE COLOR MOLECULE HAS A POSITIVE CHARGE
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BASIC DYES (+CHARGE)
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USED IN DIAGNOSIS OF TUBERCULOSIS
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ACID-FAST STAIN
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INVOLVES THE USE OF A NEGATIVE STAIN MADE FROM INDIA INK PARTIVLES
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ACIDIC DYES (-CHARGE)
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USES MALACHITE GREEN AND APPLICATION OF STEAM
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ENDOSPORE STAIN
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REFLECTS THE CHEMICAL DIFFERENCE BETWEEN BACTERIAL CELL WALLS; DIFFERENTIAL STAIN
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GRAM STAIN
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CONTAINS ORGANIC SUBSTANCES AND COMPLEX GROWTH FACTORS, SUCH AS BLOOD
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ENRICHED MEDIA
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DESIGNED TO SUPPRESS THE GROWTH OF UNWANTED BACTERIA AND TO ENCOURAGE GROWTH OF DESIRED MICROBES
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SELECTIVE MEDIA
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INGREDIENTS SUCH AS SODIUM THIOGLYCOLATE CHEMICALLY COMBINED WITH DISSOLVED OXYGEN SUPPORTS GROWTH OF ANAEROBIC BACTERIA
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GENERAL PURPOSE MEDIA
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NUTRIENTS ARE DIGESTS OR EXTRACTS; EXACT CHEMICAL COMPOSITION VARIES LITTLE FROM BATCH TO BATCH
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COMPLEX (NONSYNTHETIC) MEDIA
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GROWS MOST NONFASTIDIOUS BACTERIA
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GENERAL PURPOSE MEDIA
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THE GROWTH OF SEVERAL BACTERIAL TYPES ARE READILY DISTINGUSHED FROM EACH OTHER BASED ON COLOR CHANGES IN THE MEDIA, GROWTH OF DIFFERENT COLORED COLONIES. ECT.
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DIFFERENTIAL MEDIA
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IN 1668, DEMONSTRATED THAT MAGGOTS APPEARED ONLY IN DECAYING MEAT THAT HAD BEEN EXPOSED TO FLIES
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FRANCESCO REDI
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INTRODUCED THE CONCEPT THAT LIVING CELLS ARISE FROM OTHER LIVING CELLS, BIOGENESIS RABIES VACCINE
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LOUIS PASTUER
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VIEWED AND SKETCHED ANIMALCULES WITH HIS SIMPLE MICROSCOPE
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ANTON VAN LEEUWENHOCK
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A SURGEON WHO USED CARBOLIC ACID TO CONTROL WOUND INFECTIONS. ASEPTIC TECHNIQUE
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JOSEPH LISTER
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DEMONSTRATED THAT INFECTIONS IN OBSTETRICAL WARDS COULD BE MINIMIZED BY DISINFECTING THE HANDS OF PHYSICIANS
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IGNAZ SEMMELWEIZ
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USING ANTHRAX AS A MODEL, DEMONSTRATED THAT A SPECIFIC MICROORGANISM IS THE CAUSE OF A SPECIFIC DISEASE, POSTULATES FORMED
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ROBERT KOCH
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ORIGINATE OUR SYSTEM OF SCIENTIFIC NOMENCLATURE
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CARLOUS LINNAEUS (CARL VON LINNE)
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DEMONSTRATED HEATING DECREASED MICROBES BUT DID NOT RENDER COMPLETE STERILITY
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JOHN TYNDALL
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DISCOVERED BACTERIAL ENDOSPORES WERE HEAT RESISTANT
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FERDINARD COHN
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DEVELOPED A CLASSIFICATION SYSTEM THAT GROUPS ORGANISMS BY THEIR CELLULAR ORGANIZATION
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CARL WOESE
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DISCOVERED PENICILLIN GROWING ON CULTURE PLATES INHIBITED THE GROWTH OF BACTERIA
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ALEXANDER FLEMING
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WORKED WITH BACTERIAL CULTURE TECHNIQUES, TB AND ANTHRAX MICROBES
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ROBERT KOCH
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ALSO CALLED BLUE GREEN ALGAE; MAY FIX NITROGEN FROM AIR.
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CYANOBACTERIA
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PHOTOSYNTHETIC EUCARYOTES
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ALGAE
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EUCARYOTES CLASSIFIED PRIMARILY BY THEIR MEANS OF LOCOMOTION
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PROTOZOA
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SOURCE OF ANTIBIOTIC, INCLUDES MOLDS AND YEASTS
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FUNGI
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GENERAL NAME FOR A ROD-SHAPED BACTERIUM
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BACILLUS
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GENERAL NAME FOR A SPHERICAL BACTERIUM
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COCCUS
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STUDIES PROTECTIVE SUBSTANCES AND CELLS PRODUCED IN RESPONSE TO INFECTION
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IMMUNOLOGY
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The branch of botany dealing with algae.
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PHYCOLOGY
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THE STUDY OF PARASITES AND PARASITIC ORGANISMS.
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PARASITOLOGY
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STUDY OF FUNGI
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MYCOLOGY
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STUDY OF VIRUSES
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VIROLOGY
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STUDY OF RELATIONSHIPS BETWEEN MICROBES AND THE ENVIRONMENT
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ECOLOGY
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STUDY AND CONTROL THE SPREAD OF DISEASES
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EPIDEMIOLOGY
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STUDY OF BACTERIA
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BACTERIOLOGY
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microscope used to view objects smaller than 0.2um, such as viruses
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electron microscope
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microscope used to view heat fixed stained bacterial cells
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bright field or compound
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microscope used to view microorganisms that cdan't be heated and or stained by standard methods
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dark field
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microscope to view the internal structure of living organisms
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phase-contrast
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microscope that scans the surface features of an object
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scanning electron microscope
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