Helminths – Microbiology Answers – Flashcards

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Ascaris lumbricoides ID methods
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egg in trichrome stain from stool sample
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Ascaris lumbricoides diagnosis
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egg is 55-40 uM, if fertilized it has a thick albumin coat, if non fertilized egg has a thin shell, worms are 10-30cm coming out of the anus
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Ascaris lumbricoides special characteristics
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most common in the world, looks like earth worm, nematode
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Enterobius vermicularis ID methods
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scotch tape prep to see eggs and larvae, worms can be seen in urine
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Enterobius vermicularis diagnosis
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egg has flattened side, 55-25 uM, worms are small
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Enterobius vermicularis special characteristics
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most common in US, pinworms, fecal/oral transmission, ingest the eggs they turn into larvae and then worms in intestine, females lay eggs outside the anus, nematode
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Trichuris trichiura ID methods
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egg in feces by trichrome
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Trichuris trichiura diagnosis
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2 hyaline plugs on eggs, 15-25 uM
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Trichuris trichiura special characteristics
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whip worm, fecal oral route transmission, diarrhea, rectal prolapsed = heavy with infestation, seen in United States, nematode
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Ancyclostoma duodenale/Necator americanus ID methods
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trichrome stain with eggs in stool, serology
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Ancyclostoma duodenale/Necator americanus diagnosis
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transparent egg with colored insides
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Ancyclostoma duodenale/Necator americanus special characteristics
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hook worms, eggs look identical in both species, fecal cutaneous transmission; infected larvae can enter through skin, suckers attach to intestine, larvae are infective = filariform, non- infective = rhabidopform, nematode
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Strongyloides sterocoralis ID methods
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larvae in feces and sputum, direct prep with iodine
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Strongyloides sterocoralis diagnosis
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short buccal cavity, transparent worm
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Strongyloides sterocoralis special characteristics
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transmitted fecal cutaneous route, complete life cycle in intestine, nematode
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Trichinella spiralis ID methods
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tissue biopsy of muscle
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Trichinella spiralis diagnosis
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encysted in muscle tissue
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Trichinella spiralis special characteristics
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ingesting undercooked pork, achy, can get into heart and cause an MI, nematode
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Toxocara canes/cati and braziliense/caninum ID methods
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eggs seen in O&P, serology or tissue biopsy
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Toxocara canes/cati and braziliense/caninum diagnosis
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larvae migrate through tissue, nematode
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Toxocara canes/cati and braziliense/caninum special characteristics
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cat and dog feces, ingest eggs, visceral larval migrans and cutaneous larval migrans, nematode
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Wuchereria bancrofti ID methods
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Blood smears, thick and thin
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Wuchereria bancrofti diagnosis
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sheath +, nuclei not in tail
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Wuchereria bancrofti special characteristics
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mosquito vector, elephantitis, blocks lymphs = edema, treadlike filarial, nematode, micro-filarial
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Loa Loa ID methods
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Blood smears, thick and thin
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Loa Loa diagnosis
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sheath +, 200-300 uM, continuous nuclei in tail
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Loa Loa special characteristics
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fly bite, eye worm, nematode, micro-filarial
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Onchocerca volvulus ID methods
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Blood smears, thick and thin
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Onchocerca volvulus diagnosis
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sheath -, no terminal nuclei
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Onchocerca volvulus special characteristics
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river blindness, South America, fly cute, nematode, micro-filarial
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Brugia malayi/timori ID Methods
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Blood smears, thick and thin
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Brugia malayi/timori diagnosis
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sheath +, discontinuous nuclei, 2 nuclei in tail
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Brugia malayi/timori special characteristics
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mosquito, nematode, micro-filarial
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Taenia solium/saginata ID methods
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proglotids (segments) in stool or ID egg
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Taenia solium/saginata diagnosis
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radial striations around the egg, 35-45 uM
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Taenia solium/saginata special characteristics
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pork and beef tapeworm, embedded in nucleus, differentiate via uterine branches (T. saginata has more), ingest proglotids, ingest with India ink and count uterine branches, consume human good, 10 m long, Cestode
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Diphyllobothrium latum ID methods
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eggs in feces, proglotids in feces
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Diphyllobothrium latum diagnosis
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egg has operculum and knob at terminal end, small uterine branches, egg is 70-45uM
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Diphyllobothrium latum special characteristics
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undercooked fish, vitamin B12 deficiency, Cestode
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Hymenolepsis nana/ diminuta ID methods
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eggs in feces
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Hymenolepsis nana/ diminuta diagnosis
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colorless shell, 3 hooklets inside eggs, 50-30uM
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Hymenolepsis nana/ diminuta special characteristics
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dwarf, Cestode
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Echinococcus granulosus ID methods
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serology, biopsy cysts
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Echinococcus granulosus diagnosis
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3 proglotids, larvae in tissue
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Echinococcus granulosus special characteristics
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hydatid cyst disease, dog feces, if cysts burst can cause anaphylactic shock, not in stool, Cestode
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Fasciolopsis buski ID methods
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egg in feces, trichrome, direct or concentrated
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Fasciolopsis buski diagnosis
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operculum on egg, 150-90 uM
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Fasciolopsis buski special characteristics
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fresh water plants contain larvae, intestinal biopsy for worm, Trematode
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Clonorchis sinensis ID methods
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egg in feces
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Clonorchis sinensis diagnosis
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small egg, operculum and knob, looks like a lemon
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Clonorchis sinensis special characteristics
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Chinese liver fluke from undercooked fish, snail vector, liver biopsy, Trematode
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Fasciola hepatica ID methods
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egg in feces
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Fasciola hepatica diagnosis
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operculum without knob
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Fasciola hepatica special characteristics
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liver fluke, water plants, liver biopsy, Trematode
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Paragonimus westermani ID methods
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egg in stool
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Paragonimus westermani diagnosis
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operculum, 85-55uM, thick posterior
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Paragonimus westermani special characteristics
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fresh water crabs, lung fluke, tropica climate, Trematode
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Schistosoma mansoni/japonicum/haematobium ID methods
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egg in stool
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Schistosoma mansoni/japonicum/haematobium diagnosis
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travel in pairs, S. mansoni egg has lateral spine, s. haematobium egg has terminal spine and s. japonicum has no spine, has knob and tiny lateral spine, Trematode
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Schistosoma mansoni/japonicum/haematobium special characteristics
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infected by cercaria invading skin, fecal cutaneous route, snail, fresh water, s. haematobium egg can be seen in urine
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anacyclostoma braziliense and caninum worm
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ascaris lumbricoides egg
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ascaris lumbricoides worm
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Clonorchis sinensis egg
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Clonorchis sinensis fluke
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Diphyllabothrium latum egg
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Diphyllabothrium latum worm
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Echinococcus granulosus Hydatid cysts
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Echinococcus granulosus worm
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Enterobuis vernicularis egg
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enterobuis vernicularis worm
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Fasciola Hepatica egg
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Fasciola hepatica fluke
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Fasciolopsis buski egg
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fasciolopsis buski fluke
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hook worm egg
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Hymenolepsis egg
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Hymenolepsis worm
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Loa Loa worm
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Necator americanis and anacyclostoma duodenale larvae
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Necator americanis and anacyclostoma duodenale worm
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oncherca volulus worm
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Paragonimus westermani egg
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Paragonimus westermani fluke
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Schitsoma cercaria
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Schitsoma egg
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Schitsoma worm
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Strongyloides sterocroalis egg
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strongyloides stercoralis worm
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T. saginata worm
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T. solium worm
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Taenia egg
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Taenia scolex
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Toxocara canis and cati egg
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Trichinella spiralis
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Trichuris trichiura egg
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trichuria trichiura worm
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Wuchereria bancrofti
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