Quant. Anal. Chp 18 Spectromet – Flashcards
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[Dual Nature of Light] Wave: |
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-Explains physical properties of light itself. -Physicist |
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[Dual Nature of Light] Particle (Photon): |
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-Explains how light interacts with matter. -Biologist/Chemist |
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1. Relation between frequency and wavelength:
2. Speed of light in other medium? |
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1. c= νλ
2. c/n |
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Relation between energy and frequency (or wavelength): |
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What are the ways light interacts with matter? |
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Light can be:
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Absorption- |
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Transfer of energy from photon to atom or molecule which produces transition from a lower energy to a higher energy level. |
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Emission- |
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Production of photon of energy from atom or molecule which is originally in a higher energy level and which returns atom or molecule to a lower energy level. |
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What are the 5 main components of a spectrophotometric experiment? |
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[image] |
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Transmittance: |
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Absorbance: |
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Beer-Lambert Law: |
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Aλ=ελbc
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Limitations of Beer-Lambert Law: |
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Common Cuvets for Ultraviolet and Visible Measurements: |
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