Unit 1 Lab – Flashcards
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Unlock answersWhy was it necessary to move the letter "e" to the center of the low power field before changing to high power?
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| to keep the image in your field of view |
| How does the microscope change the position of the object being observed? |
| The object is upside down and backwards in orientation. |
| What is the total magnification of objects observed through a 40x high power objective with a 7.5x ocular? |
| 300X |
| If you were getting a total magnification of 270x while using a 45x objective, what would be the power of the eyepiece? |
| 6x |
| Trace the path of light through a microscope numbering each part of the microscope as the light passes through from the light to the oculars. |
1. light source 2. diaphragm 3. condenser 4. stage 5. objectives 6. revolving nose piece 7. body tube 8. ocular |
| To bring your specimen into focus when using the high power objective, which focusing knob should you use? |
| fine adjustment |
| Explain this statment: With high power you see more of less |
| You see less of the slide area but more specific detail |
Base: microscope rests on this part Coarse adjustment: used to initially focus the microscope Ocular: magnification and viewing of the specimen Revolving Nose Piece: hold the objectives and allows you to change magnifications Stage: a platform that holds the specimen/slide for viewing Condenser: directs the path of light up into a straighter, more concentrated beam that enters into the lenses of the microscope |
Diaphragm: regulates the amount of light entering into the lenses of the microscope Objectives: magnification Arm: support the upper part of the microscope Light: provides illumination fo the specimen Body Tube: holds the oculars and directs the light to the eye Stage Clips: hold the speimen/slide in place |
| The smallest distance between two objects that allows the objects to be distinguished as two separate objects and not as one |
| resolution |
| As magnification increases |
| the field of view decreases |
| Magnification |
| is due to the outward bending of light rays as they pass through a lens |
| Once a microscope has been initially focused, the ability of this scope to stay focused when changing to a higher magnification is |
| parfocal |
| The actual amount of area on a slide that can be viewed under the microscope at one time without having to move the slide |
| field of view |
| The distance from the specimen on the slide to the objective lens of the microscope, when the specimen is in focus |
| working distance |
