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Adrien Vincent
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Livia Baldwin
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Forensic Science Increased Heart Rate Psychology Thin Layer Chromatography True And False
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James Storer
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Richard Molina
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Compounds Thin Layer Chromatography
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Judith Simpson
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Identify The Components Molecules Thin Layer Chromatography
Experiment Three: Separation and Identification of Food Dyes by Paper Chromatography 15 terms
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Christine Brunetti
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A Level Biology Thin Layer Chromatography
paper chromatography -identify unknown amino acids 17 terms
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Tiffany Hanchett
17 terms
During Thin Layer Chromatography, what method is typically used to apply the dilute solution of the sample to a TLC plate?
A thin glass spotter or capillary tube
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What does Thin Layer Chromatography use for the stationary and mobile phases?
-Stationary phase: A plate of glass (flat, unreactive surface). -Mobile Phase: Silica/Aluminia (thin layer of an inert substance)
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What are paper and thin layer chromatography based on?
The differential partitioning of the samples (solutes) between a polar stationary phase and a nonpolar mobile phase.
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What are paper and thin layer chromatography often used for and why?
They are often used for the initial screening of urine samples for drug metabolites because they are inexpensive and quick.
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In the phase transfer catalysis experiment (10), what type of analysis was used to determine the progress of the reaction? A) Extraction B) Thin Layer Chromatography C) Gas Chromatography D) Distillation E) Recrystallization
B) Thin Layer Chromatography
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THIN LAYER CHROMATOGRAPHY uses:
TLC (abbreviated to) is widely used by chemists to assess the progress of reactions and purity of products
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THIN LAYER CHROMATOGRAPHY general info:
Stationary phase of TLC is a thn layer of solid, e.g. silica gel. Mobile phase of TLC is an organic solvent
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In thin layer chromatography (TLC) , the distance the solute migrates divided by the distance the solvent migrates is the : A. tRB. KdC. RfD. pK
C. Rf
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Explanation of events in THIN LAYER CHROMATOGRAPHY
1) Small spots of mixture to be seperated are added to TLC plate 2) Plate is then dipped in organic solvent WITHOUT immersing the spots of the mixture 3) As solvent moves up plate components of mixture are carried up a certain distance as well (depending on factors) 4) Migration up the plate of the spot is slowed by adsorption of the components to the stationary phase (thin layer of solid)
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Thin Layer Chromatography (TLC)
is a specialized form of chromatography. It is a simple, sensitive and quick technique for analyzing and separating ultra small quantities of organic compounds. It is used specifically to check the purity of a compound, to determine the number of components in a mixture, to evaluate the progess of a chemical reaction, and to analyze the composition of a mixture.
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