During Thin Layer Chromatography, what method is typically used to apply the dilute solution of the sample to a TLC plate?

A thin glass spotter or capillary tube More test answers on https://studyhippo.com/chem-237-2/

What does Thin Layer Chromatography use for the stationary and mobile phases?

-Stationary phase: A plate of glass (flat, unreactive surface). -Mobile Phase: Silica/Aluminia (thin layer of an inert substance) More test answers on https://studyhippo.com/c7-chromatography/

What are paper and thin layer chromatography based on?

The differential partitioning of the samples (solutes) between a polar stationary phase and a nonpolar mobile phase. More test answers on https://studyhippo.com/paper-chromatography/

What are paper and thin layer chromatography often used for and why?

They are often used for the initial screening of urine samples for drug metabolites because they are inexpensive and quick. More test answers on https://studyhippo.com/paper-chromatography/

In the phase transfer catalysis experiment (10), what type of analysis was used to determine the progress of the reaction? A) Extraction B) Thin Layer Chromatography C) Gas Chromatography D) Distillation E) Recrystallization

B) Thin Layer Chromatography More test answers on https://studyhippo.com/chemistry-231-exam/

THIN LAYER CHROMATOGRAPHY uses:

TLC (abbreviated to) is widely used by chemists to assess the progress of reactions and purity of products More test answers on https://studyhippo.com/chromatography/

THIN LAYER CHROMATOGRAPHY general info:

Stationary phase of TLC is a thn layer of solid, e.g. silica gel. Mobile phase of TLC is an organic solvent More test answers on https://studyhippo.com/chromatography/

Explanation of events in THIN LAYER CHROMATOGRAPHY

1) Small spots of mixture to be seperated are added to TLC plate 2) Plate is then dipped in organic solvent WITHOUT immersing the spots of the mixture 3) As solvent moves up plate components of mixture are carried up a certain distance as well (depending on factors) 4) Migration up the plate of the spot is slowed by adsorption of the components to the stationary phase (thin layer of solid) More test answers on https://studyhippo.com/chromatography/

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Thin Layer Chromatography Flashcards, test questions and answers

Q: Thin Layer Chromatography (TLC)

is a specialized form of chromatography. It is a simple, sensitive and quick technique for analyzing and separating ultra small quantities of organic compounds. It is used specifically to check the purity of a compound, to determine the number of components in a mixture, to evaluate the progess of a chemical reaction, and to analyze the composition of a mixture. More test answers on https://studyhippo.com/chemistry-lab-final-exam-17732/

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What is Thin Layer Chromatography?

Thin Layer Chromatography (TLC) is a powerful analytical technique used to separate and identify components of a mixture. It is an inexpensive and fast method of separating, identifying and quantifying components in a variety of mixtures. TLC is often used in the analysis of various compounds such as drugs, pesticides, amino acids, fatty acids, vitamins, carbohydrates and proteins.TLC works by taking advantage of the fact that different compounds have different affinity for a stationary phase (usually silica gel or alumina) that is coated on a glass plate. A sample solution containing the compounds of interest will be applied to the plate in either liquid or vapor form. The compound will then travel across the plate due to capillary action caused by differences in polarity between the stationary phase and mobile phase (solvent). Different compounds move at different rates depending on their respective affinities for the stationary phase which allows them to be separated from one another.Once separation has occurred, identification can be made by comparing retention times with reference standards as well as observing any visible spots under UV light or other forms of visualization methods. Quantification can also be determined if calibration curves are created prior to analysis using solutions containing known concentrations of each component in question. Additionally, TLC can also be used to confirm purity or detect impurities in samples by comparing chromatograms obtained from pure samples with those that contain unknowns. In conclusion, Thin Layer Chromatography offers many advantages over other techniques such as its low cost, speed and relative simplicity making it an attractive technique for many applications including drug discovery and quality control laboratories around the world.