Test 1- Gram Pos Cocci – Flashcards

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question
What part of the cell differentiates gram positive from gram negative organisms?
answer
the cell wall
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What kind of bacterial walls contain peptidoglycan?
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All bacteria except Mycoplasma
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What kind of bacterial cell walls contain teichoic acids?
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Gram positive bacteria only
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What kind of bacterial cell walls contain lipopolysaccharide?
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Gram negative bacteria only
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Which has a thicker cell wall- gram positive or gram negative bacteria?
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Gram positive
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How many layers are in a gram positive cell wall?
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2
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How many layers are in a gram negative cell wall?
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3
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Which kind of bacterial cell wall has more peptidoglycan?
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Gram positive has more than 50% while gram neg has 10-20% peptidoglycan
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Which bacteria are sensitive to penicillin- gram positive or gram negative?
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Gram positive bacteria are sensitive to penicillin
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Which kind of bacteria contain protein- gram negative or gram positive?
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gram negative
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What color do gram positive bacteria stain?
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purple
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What color do gram negative bacteria stain?
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pink
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What are the two types of pili?
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common pili and sex or F pili
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what kind of pili are multiple on cell surface and used for attachment?
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common pili
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What does it mean if a bacteria is F+?
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Bacteria that carry the F plasmid are F+. The F plasmid encodes enzymes and proteins that form the sex pilus
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What word describes a single flagellum at one end?
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Monotrichous
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Small bunches of flagella arising from one end of the cell
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Lophotrichous
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Having flagella at both ends of the cell
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Amphitrichous
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Having flagella dispersed over the surface of the cell
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Peritrichous
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What flagellar arrangement results in the slowest motility?
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Peritrichous
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How does the capsule protect the bacterial cell?
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by blocking phagocytosis
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What is the function of plasmids?
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to transfer genetic information between organisms
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bacterial transmission from person to person
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direct- horizontal
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bacterial transmission from mother to baby
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direct- vertical
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transmission of bacteria by a vehicle, airborne, or vector-borne
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indirect
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Four bacterial virulence properties
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capsule formation, enzyme production, adherence factors, toxin production
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what kind of toxins are produced by gram negative bacteria?
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endotoxins
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when are endotoxins released?
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when the cell lyses
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what kind of bacteria releases exotoxins?
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gram positive
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when are exotoxins released?
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exotoxins are released by living bacteria to target specific host cells
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bacteria that require oxygen to live
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obligate aerobes
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bacteria that will only grow when oxygen is present in amounts below 0.2 atm
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microaerophilic
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bacteria that will only grow when oxygen is not present
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obligate anaerobes
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bacteria that do not require oxygen for growth but utilize it when it's available
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facultative anaerobe
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Is oxygen required for fermentation?
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no
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what is produced during fermentation?
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high concentration of strong acids
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what are the 5 types of media?
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Supportive, selective, differential, enrichment, enriched
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what kind of media contains general nutrients, supports the growth of non-fastidious bacteria, and does not give one organism an advantage?
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supportive media
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Nutrient broth/agar, trypticase soy agar, and luria burtoni are examples of what kind of media?
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supportive media
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what kind of media contains inhibitory agents such as dyes, bile salts, alcohol, acid or antibiotics?
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selective media
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mannitol salt agar, hektoen-enteric agar, phenylethyl alcohol agar and campy blood agar are all examples of what kind of media?
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selective media
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what kind of media has distinguishing factors to differentiate bacteria?
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Differential media
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MacConkey agar, Mannitol-salt agar, blood agar and triple sugar iron agar are all examples of what kind of media?
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differential media
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what kind of media contains required nutrients for one particular pathogen?
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enrichment media
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buffered charcoal yeast extract agar, enriched thayer martin agar, and selonite broth are all examples of what kind of media?
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enrichment media
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what kind of media contains factors to aid growth of fastidious organisms?
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enriched media
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blood agar and chocolate agar are both examples of what kind of media?
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enriched media
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What is the first test used to differentiate staphylococcus species?
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coagulase test
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What test differentiates between streptococcus and staphylococcus?
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catalase test
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Is Staph aureus coagulase positive or negative?
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coagulase positive
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What are the two coagulase negative staphylococci most likely to cause disease?
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S. epidermidis and S. saprophyticus
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What is the most virulent Staph species?
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S. aureus
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What is the function of techoic acid?
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It mediates adherence as part of the cell wall
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Does Staph aureus ferment mannitol?
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yes.
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When Staph aureus is grown on mannitol agar, what color is produced?
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yellow
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What kind of hemolysis would you see with Staph aureus?
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Beta hemolysis
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What kind of hemolysis would you see with Staph aureus?
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Beta hemolysis
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What is hyaluronidase?
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virulence factor of staph aureus that digests connective tissue- spreading factor
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What is staphylokinase?
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virulence factor of s. aureus- enzyme that digests blood clots
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What gene is responsible for methicillin resistant staph aureus?
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mec gene
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Which staph species causes urinary tract infections?
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Staphylococcus saprophyticus
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What test uses H2O2 to see if the organism changes it into H2O and O2?
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Catalase test
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Which staph species is resistant to novobiocin?
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staph saprophyticus. all others are sensitive
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What group is Streptococcus pyogenes?
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Group A
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What group is Streptococcus agalactiae?
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Group B
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What are the two categories of Group D Strep?
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Enterococcus and nonenterococcal Group D
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