Test 1- Gram Pos Cocci – Flashcards
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What part of the cell differentiates gram positive from gram negative organisms? |
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the cell wall |
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What kind of bacterial walls contain peptidoglycan? |
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All bacteria except Mycoplasma |
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What kind of bacterial cell walls contain teichoic acids? |
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Gram positive bacteria only |
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What kind of bacterial cell walls contain lipopolysaccharide? |
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Gram negative bacteria only |
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Which has a thicker cell wall- gram positive or gram negative bacteria? |
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Gram positive |
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How many layers are in a gram positive cell wall? |
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2 |
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How many layers are in a gram negative cell wall? |
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3 |
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Which kind of bacterial cell wall has more peptidoglycan? |
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Gram positive has more than 50% while gram neg has 10-20% peptidoglycan |
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Which bacteria are sensitive to penicillin- gram positive or gram negative? |
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Gram positive bacteria are sensitive to penicillin |
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Which kind of bacteria contain protein- gram negative or gram positive? |
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gram negative |
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What color do gram positive bacteria stain? |
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purple |
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What color do gram negative bacteria stain? |
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pink |
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What are the two types of pili? |
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common pili and sex or F pili |
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what kind of pili are multiple on cell surface and used for attachment? |
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common pili |
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What does it mean if a bacteria is F+? |
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Bacteria that carry the F plasmid are F+. The F plasmid encodes enzymes and proteins that form the sex pilus |
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What word describes a single flagellum at one end? |
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Monotrichous |
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Small bunches of flagella arising from one end of the cell |
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Lophotrichous |
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Having flagella at both ends of the cell |
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Amphitrichous |
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Having flagella dispersed over the surface of the cell |
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Peritrichous |
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What flagellar arrangement results in the slowest motility? |
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Peritrichous |
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How does the capsule protect the bacterial cell? |
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by blocking phagocytosis |
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What is the function of plasmids? |
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to transfer genetic information between organisms |
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bacterial transmission from person to person |
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direct- horizontal |
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bacterial transmission from mother to baby |
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direct- vertical |
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transmission of bacteria by a vehicle, airborne, or vector-borne |
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indirect |
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Four bacterial virulence properties |
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capsule formation, enzyme production, adherence factors, toxin production |
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what kind of toxins are produced by gram negative bacteria? |
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endotoxins |
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when are endotoxins released? |
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when the cell lyses |
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what kind of bacteria releases exotoxins? |
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gram positive |
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when are exotoxins released? |
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exotoxins are released by living bacteria to target specific host cells |
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bacteria that require oxygen to live |
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obligate aerobes |
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bacteria that will only grow when oxygen is present in amounts below 0.2 atm |
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microaerophilic |
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bacteria that will only grow when oxygen is not present |
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obligate anaerobes |
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bacteria that do not require oxygen for growth but utilize it when it's available |
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facultative anaerobe |
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Is oxygen required for fermentation? |
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no |
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what is produced during fermentation? |
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high concentration of strong acids |
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what are the 5 types of media? |
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Supportive, selective, differential, enrichment, enriched |
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what kind of media contains general nutrients, supports the growth of non-fastidious bacteria, and does not give one organism an advantage? |
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supportive media |
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Nutrient broth/agar, trypticase soy agar, and luria burtoni are examples of what kind of media? |
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supportive media |
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what kind of media contains inhibitory agents such as dyes, bile salts, alcohol, acid or antibiotics? |
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selective media |
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mannitol salt agar, hektoen-enteric agar, phenylethyl alcohol agar and campy blood agar are all examples of what kind of media? |
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selective media |
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what kind of media has distinguishing factors to differentiate bacteria? |
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Differential media |
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MacConkey agar, Mannitol-salt agar, blood agar and triple sugar iron agar are all examples of what kind of media? |
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differential media |
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what kind of media contains required nutrients for one particular pathogen? |
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enrichment media |
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buffered charcoal yeast extract agar, enriched thayer martin agar, and selonite broth are all examples of what kind of media? |
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enrichment media |
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what kind of media contains factors to aid growth of fastidious organisms? |
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enriched media |
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blood agar and chocolate agar are both examples of what kind of media? |
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enriched media |
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What is the first test used to differentiate staphylococcus species? |
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coagulase test |
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What test differentiates between streptococcus and staphylococcus? |
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catalase test |
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Is Staph aureus coagulase positive or negative? |
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coagulase positive |
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What are the two coagulase negative staphylococci most likely to cause disease? |
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S. epidermidis and S. saprophyticus |
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What is the most virulent Staph species? |
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S. aureus |
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What is the function of techoic acid? |
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It mediates adherence as part of the cell wall |
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Does Staph aureus ferment mannitol? |
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yes. |
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When Staph aureus is grown on mannitol agar, what color is produced? |
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yellow |
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What kind of hemolysis would you see with Staph aureus? |
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Beta hemolysis |
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What kind of hemolysis would you see with Staph aureus? |
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Beta hemolysis |
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What is hyaluronidase? |
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virulence factor of staph aureus that digests connective tissue- spreading factor |
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What is staphylokinase? |
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virulence factor of s. aureus- enzyme that digests blood clots |
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What gene is responsible for methicillin resistant staph aureus? |
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mec gene |
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Which staph species causes urinary tract infections? |
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Staphylococcus saprophyticus |
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What test uses H2O2 to see if the organism changes it into H2O and O2? |
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Catalase test |
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Which staph species is resistant to novobiocin? |
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staph saprophyticus. all others are sensitive |
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What group is Streptococcus pyogenes? |
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Group A |
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What group is Streptococcus agalactiae? |
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Group B |
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What are the two categories of Group D Strep? |
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Enterococcus and nonenterococcal Group D |