Test 1- Gram Pos Cocci – Flashcards
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| What part of the cell differentiates gram positive from gram negative organisms? |
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| the cell wall |
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| What kind of bacterial walls contain peptidoglycan? |
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| All bacteria except Mycoplasma |
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| What kind of bacterial cell walls contain teichoic acids? |
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| Gram positive bacteria only |
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| What kind of bacterial cell walls contain lipopolysaccharide? |
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| Gram negative bacteria only |
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| Which has a thicker cell wall- gram positive or gram negative bacteria? |
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| Gram positive |
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| How many layers are in a gram positive cell wall? |
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| 2 |
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| How many layers are in a gram negative cell wall? |
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| 3 |
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| Which kind of bacterial cell wall has more peptidoglycan? |
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| Gram positive has more than 50% while gram neg has 10-20% peptidoglycan |
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| Which bacteria are sensitive to penicillin- gram positive or gram negative? |
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| Gram positive bacteria are sensitive to penicillin |
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| Which kind of bacteria contain protein- gram negative or gram positive? |
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| gram negative |
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| What color do gram positive bacteria stain? |
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| purple |
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| What color do gram negative bacteria stain? |
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| pink |
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| What are the two types of pili? |
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| common pili and sex or F pili |
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| what kind of pili are multiple on cell surface and used for attachment? |
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| common pili |
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| What does it mean if a bacteria is F+? |
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| Bacteria that carry the F plasmid are F+. The F plasmid encodes enzymes and proteins that form the sex pilus |
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| What word describes a single flagellum at one end? |
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| Monotrichous |
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| Small bunches of flagella arising from one end of the cell |
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| Lophotrichous |
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| Having flagella at both ends of the cell |
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| Amphitrichous |
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| Having flagella dispersed over the surface of the cell |
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| Peritrichous |
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| What flagellar arrangement results in the slowest motility? |
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| Peritrichous |
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| How does the capsule protect the bacterial cell? |
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| by blocking phagocytosis |
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| What is the function of plasmids? |
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| to transfer genetic information between organisms |
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| bacterial transmission from person to person |
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| direct- horizontal |
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| bacterial transmission from mother to baby |
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| direct- vertical |
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| transmission of bacteria by a vehicle, airborne, or vector-borne |
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| indirect |
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| Four bacterial virulence properties |
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| capsule formation, enzyme production, adherence factors, toxin production |
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| what kind of toxins are produced by gram negative bacteria? |
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| endotoxins |
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| when are endotoxins released? |
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| when the cell lyses |
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| what kind of bacteria releases exotoxins? |
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| gram positive |
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| when are exotoxins released? |
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| exotoxins are released by living bacteria to target specific host cells |
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| bacteria that require oxygen to live |
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| obligate aerobes |
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| bacteria that will only grow when oxygen is present in amounts below 0.2 atm |
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| microaerophilic |
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| bacteria that will only grow when oxygen is not present |
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| obligate anaerobes |
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| bacteria that do not require oxygen for growth but utilize it when it's available |
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| facultative anaerobe |
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| Is oxygen required for fermentation? |
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| no |
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| what is produced during fermentation? |
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| high concentration of strong acids |
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| what are the 5 types of media? |
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| Supportive, selective, differential, enrichment, enriched |
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| what kind of media contains general nutrients, supports the growth of non-fastidious bacteria, and does not give one organism an advantage? |
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| supportive media |
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| Nutrient broth/agar, trypticase soy agar, and luria burtoni are examples of what kind of media? |
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| supportive media |
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| what kind of media contains inhibitory agents such as dyes, bile salts, alcohol, acid or antibiotics? |
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| selective media |
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| mannitol salt agar, hektoen-enteric agar, phenylethyl alcohol agar and campy blood agar are all examples of what kind of media? |
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| selective media |
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| what kind of media has distinguishing factors to differentiate bacteria? |
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| Differential media |
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| MacConkey agar, Mannitol-salt agar, blood agar and triple sugar iron agar are all examples of what kind of media? |
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| differential media |
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| what kind of media contains required nutrients for one particular pathogen? |
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| enrichment media |
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| buffered charcoal yeast extract agar, enriched thayer martin agar, and selonite broth are all examples of what kind of media? |
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| enrichment media |
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| what kind of media contains factors to aid growth of fastidious organisms? |
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| enriched media |
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| blood agar and chocolate agar are both examples of what kind of media? |
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| enriched media |
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| What is the first test used to differentiate staphylococcus species? |
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| coagulase test |
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| What test differentiates between streptococcus and staphylococcus? |
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| catalase test |
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| Is Staph aureus coagulase positive or negative? |
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| coagulase positive |
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| What are the two coagulase negative staphylococci most likely to cause disease? |
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| S. epidermidis and S. saprophyticus |
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| What is the most virulent Staph species? |
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| S. aureus |
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| What is the function of techoic acid? |
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| It mediates adherence as part of the cell wall |
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| Does Staph aureus ferment mannitol? |
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| yes. |
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| When Staph aureus is grown on mannitol agar, what color is produced? |
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| yellow |
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| What kind of hemolysis would you see with Staph aureus? |
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| Beta hemolysis |
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| What kind of hemolysis would you see with Staph aureus? |
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| Beta hemolysis |
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| What is hyaluronidase? |
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| virulence factor of staph aureus that digests connective tissue- spreading factor |
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| What is staphylokinase? |
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| virulence factor of s. aureus- enzyme that digests blood clots |
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| What gene is responsible for methicillin resistant staph aureus? |
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| mec gene |
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| Which staph species causes urinary tract infections? |
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| Staphylococcus saprophyticus |
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| What test uses H2O2 to see if the organism changes it into H2O and O2? |
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| Catalase test |
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| Which staph species is resistant to novobiocin? |
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| staph saprophyticus. all others are sensitive |
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| What group is Streptococcus pyogenes? |
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| Group A |
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| What group is Streptococcus agalactiae? |
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| Group B |
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| What are the two categories of Group D Strep? |
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| Enterococcus and nonenterococcal Group D |