Prokaryotic & Eukaryotic Cell – Flashcards

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prokaryotic cell structure
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glycocalyx
- capsule (slime layer): layer of gelatinous material covering the cell.

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- composition varies with species: polypeptides, polysaccharides, glycoprotein
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function of glycocalyx (capsule or slime layer)
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- ahesion to surfaces
- osmotic barriers (protection from pressure)
- protect bacteria from phagocytosis
- storage of nutrient
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prokaryotic cells
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- pro = before karyon = kernel
- kingdom monera
- no true nucleus
- genetic material is in "nucleiod" region
- no membrane bound organelles
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flagella
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fx. locomotion/motility
- peritrichous (all around)
- amphitrichous (both ends)
- monotrichous (one end)
- lophotrichous (a group, but going at one end)
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fimbriae and pili
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- small hair-like projection
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function of fimbrae and pili
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-sexual conjugation - pili form tubes for transfer of genetic material
- surface adhesion
-bacteriophage receptors - some viruses attach to pili
- NOT involved in motility
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function of cell wall
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- maintains shape of the cell
- physical protection
- enables the cell to escape rupture (lysis) in a hypotonic environment
- lattice/net-like structure
- consists of peptidoglycan units that repeat over and over
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gram positive structure
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- many layers of peptidoglycan
- forms a thick rigid structure
- contains techoic acid
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gram negative structure
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-thin layer of peptidoglycan
- looser lattice (less rigid cell wall)
- high lipid content
- no teichoic acid (largely lipid)
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plasma membrane
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- regulates diffusion in and out of cell
- contains active transport system
- site of enzymes involved in ATP production
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fluid mosaic model
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- phospholipid bilayer
- a "dynamic" arrangement of phospholipids and proteins
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chromatophores
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- contains chlorophyll
- only found in photosynthetic bacteria
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nuclear material
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- 1 chromosome (DNA) loose in the cytoplasm in the nucleoid region
- bacteria often contain small circular, double-stranded DNA molecules called plasmids.
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ribosomes
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- granules scattered throughout the cytoplasm
- protein synthesis, not membrane bound, organelle in both pro. and euk.
- protein synthesis = assemble a.a. into polypeptides
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cytoplasmic inclusions
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-accumulated stored materials, valuable stored materials
-metachromatic granules (volutin) stored form of phosphate. primary in gram negative. take up stain more readily (suck up stain fast)
- polysaccharide granules
- lipid inclusions
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magnetosomes
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-inclusions of iron oxide
- act like magnets
- may protect cells from accumulation of hydrogen peroxide
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endospores
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-highly resistant stucture
- produced by certain bacteria
- all members of the family Bacillaceae produce endospores
- e.g. Genus Bacillus and Clostridium
- Resistance : the most resistant of all living cells
- highly resistant to: dessication (drying out), heat and toxic chemicals
- low metabolic activity (dormant)
- low water content
- thick spore coat
- heat resistant chemicals (calcium and dipicolinic acid)
- formation of endospores: not a reproductive process
- vegetative cell forms a single spore
- germinates to form one vegetative cell
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eukaryotic cells
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- eu - true or good, karyon = kernel
- kingdoms: protista, plantae, fungi, animalia
- true nucleus containing genetic material
- contain membrane bound organelles
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cytoskeleton
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-network of fibers that form a dynamic framework for support and movement
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microfilaments, actin filaments, intermediate filaments
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microtubules
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- form: cilia, flagella, and centrioles
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organelles
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- specialized structures that perform specific functions
- sequester reactions
- many contain folded membranes (increased surface area)
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nucleus
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spherical or oval, contains DNA
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nuclear envelope/membrane
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- double membrane perforated by pores
- maintains nuclear shape and regulates transport in and out of the nucleus
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nucleolus
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- one or more spherical bodies within the nuclear envelope
- functions in the synthesis of ribosomal RNA
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rough ER
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- studded with ribosomes
- site for synthesis of secretory proteins and membrane molecules
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smooth ER
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- lack ribosomes
- synthesizes phospholipids, fats, and steroids
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ribosomes (euk.)
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- formed of 2 subunits: 60S and 40S
- free ribosomes:suspended in the cytoplasm
- bound ribosomes: attached to ER
- sites of protein synthesis
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golgi apparatus
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- flattened sacs, stacked on one another (pancakes)
- receive and modify products from the ER
- packages products in vesicles that transport, secretory, and storage
- lysosomes: membrane-enclosed sac of hydrolytic enzymes
- functions in phagocytosis, recycling cells own organic material
- programmed cell destruction
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lysosomal storage disease
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- lysosomes lack one of the hydrolytic enzynmes
- i.e. Tay-Sachs diseas: lack enzyme to digest lipid. excess lipid accumulates and damages brain cells
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mitochondria
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"power house of the cell"
- synthesis of ATP, sites of cellular respiration
- inner membranes folds into cristae
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plasma membrane
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-semipermeable/selectively permeable
- controls what enters and leaves the cell
- maintains the cells "homeostasis" (homo = same; stasis = standing)
- keeps the internal chemical composition within narrow limits
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transport
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the movement of materials across the plasma membrane
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passive transport
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does not require energy from cell
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simple diffusion
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molecules move from an area of greater concentraiton ot an area of lesser concentraion
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facilitated diffusion
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a carrier protein combines with a specific substance and moves it across the membrane
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osmosis
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the diffusion of water through a semipermeable membrane (water moves from a higher to lower concentration)
- water moves toward a region of higher solute (dissolved substance)concentration.
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osmoregulation
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the control of water balance
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isotonic
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-(iso = same; tonus = tension)
- concentration of dissolved substances are the same inside and outside the cell; no net flow of water
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hypertonic
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-(hyper = above/exceeding)
- high solute concentration
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hypotonic
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hypo = lower/below
- low solute concentration
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plasmoptysis
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- (osmotic lysis) bursting of a cell in a hypotonic solution n(taking up the water)
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plasmolysis
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- shrinkage of the cytoplasm due to loss of water in a hypertonic solution
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channel proteins
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proteins which form channels through the membrane allowing passage of certain molecules.
- gated channels: some can act as "gates" that open and close
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active transport
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-can move substances against their concentration gradient
- requires that the cell extend energy (ATP) e.g. sodium-potassium pump
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transport of large particles
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- endocytosis
- exocytosis
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endocytosis
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- phagocytosis (cell eating)
- pinocytosis (cell drinking)
- receptor-mediated endocytosis
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exocytosis
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-vacuole fuses with plasma membrane and expels contents from cell
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