Prokaryotic – Flashcards

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Prokaryote Cells
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  • Comes from the Greek words Prenucleus
  • One circular chromosome not in membrane
  • No Histones
  • No Organelles
  • Examples:Bacteria: peptidoglycan cell walls & Archea Pseudomurein cell walls
  • Cell division is by Binary Fision
  • No sexual recombination
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What are the charateristics of bacteria
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  • Average size 0.2 -1.0um x 2-8um
  • Most bacteria are monomorphic
  • A few are Pleomorphic
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Monomorphic
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shape doesn't change
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Pleomorphic
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Shape may change
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What are the 3 types of basic shape of bacteria
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  • Bacillus,Coccus ; Spiral
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Types of Coccus
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  • Diploccocci,Streptococci,Tetrad,Sarcinae and Staphylococci.
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Diplococci
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Remain in pairs after dividing
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Streptococci
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attached in chainlike patterns
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Tetrad
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divide in two planes and remain in groups of four
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Sarcinae
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divide in three planes remain attached in cubelike groups of eight.
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Staphylococci
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divide in multiple planes and form grapelike clusters
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Types of Bacillus
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  • single bacillus,diplobacilli,streptobacilli,coccobacillus
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Single Bacillus
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single rods
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Diplobacilli
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rods in pairs
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Streptobacilli
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rods in chains
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Coccobacillus
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oval and look like cocci
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Types of Spiral bacteria
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Have one or more twists 
  • Spirillum 
  • Vibrio
  • Spirochete 
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Spirillum
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helical shape and are rigid
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Vibrio
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curved rods
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Spirochete
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  • helical and flexible
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What type of bacteria caused the Antrax virus
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Bacillus Subtilis
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Arrangements
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  • Pairs: Diplococci,Diplobacilli
  • Clusters: Staphylococci
  • Chains: Streptococci, Streptobacilli
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Pilus
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Use to transfer DNA horizontally(within the same generation) and assists w/ attachments to surfaces.
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Fimbriae
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Main job is attachment
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Glycocalyx
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  • Outside the cell wall
  • Usually sticky
  • Capsule: neatly organized
  • Slime Layer" unorganized and loose
  • Extracelullar polysaccharide allows cell to attach
  • Capsules prevent phagocytosis
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Flagella
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  • Outside cell wall
  • made of chains of flagelin
  • Attached to a protein hook
  • Anchored to the wall and membrane by the basal body
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Gram Positive Bacteria

 

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  • Many layers of Peptidoglycan 
  • Thick, rigid structure
  • Ex: Staphilococcus Aureus
  • contains Teichoic Acids
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Gram Negative Bacteria
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  • Thin Peptidoglycan due to outer membrane presence
  • Periplasmatic space between outer membrane and cell wall
  • Ex: Eschericia Coli
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Peptidoglycan
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  • Macromolecular network composed in the bacteria's cell wall
  • Polymer of disaccharide:
  1. N. acetylglucosamine (NAG)
  2. N. acetylmuramic (NAM)
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Arrangements of Bacterial Flagella
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  • Peritrichous: Evenly distributed flagella
  • Monotrichous: A single Flagella
  • Lopotrichous:2 or more on one end
  • Amphitrichous: one on each end
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Motile Cells
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Rotate flagella to run or tumble
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Peritrichous
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Evenly Distributed Flagella
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Monotrichous
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A single Flagella
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Lophotrichous
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2 or more on one end
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Amphitricous
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1 on each end
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Taxis
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movement of bacteria from or away from stimuli
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Axial Filaments or Endoflagella
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  • found in spirochetes
  • Anchored at one end of cell
  • Rotation causes cell to move
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Fimbriae
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Allows attachment to ach other or surfaces
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Pili (Pilus)
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Facilitates transfer of DNA from one cell to another

 

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Types of Motility Associated w/ Pili
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  • Gliding Montility 
  • Twitching Motility
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Cell Wall (Prokaryotes)
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  • Prevents osmotic lysis
  • Made of Peptidoglycan(in bacteria)
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Polypeptides
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Peptidoglycan in Gram Positive bacteria is linked by Polypeptides (Peptide portion of peptidoglycan)
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Types of Teichoic Acids in Gram Positive Cell Walls
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  • Lipoteichoic acid linked to the plasma membrane 
  • Wall teichoic acid links to peptidoglycan
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Teichoic Acids
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May regulate movement of cations and

Polysaccharides provide antigenic variation

 

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Gram Negative Outer Membrane
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  • Consists of Lypopolysaccharides,lipoproteins,phospholipids.
  • forms the periplasm between the outer membrane and the plasma membrane
  • Protection from phagocytes,complement, and antibiotics
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O Polysaccharide
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Antiagen Ex E. Coli O157:H7
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Lipid A
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Is an endotoxin
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Porins
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(proteins) from channels thru membranes
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The Gram Stain Mechanism
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Crystal violet is applied then Iodine and as a result large crystals are formed

 

In Gram Positive 

Alcohol is used to dehydrate the peptidoglycan as a result crystals do not escape

 

In Gram Negative

Alcohol dissolves the outer membrane leaving holes in Peptidoglycan and crystals escape

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Thick Peptidolycan Layer Makes
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For small pores which retains capsules
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Thin Peptidoglycan Layer Makes
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For Large pores allowing crystals to escape
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Gram Positive Cell Wall
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2 ring basal body

Disrupted by lysozyme (secreted in our sebum & sweat are able to destroy Peptidoglycan cross links)

They are Penicilin sensitive

*Penicilin:kills only actively growing cells by desruting cross-linkage, cell dies by Lysis(bursts)

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Gram Negative Cell Wall
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4 ring basal body

Endotoxin

They're Tetracycline Sensitive

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Atypical Cell Walls
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No walls or little wall material

Like Mycoplasmas and Archaea

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Mycoplasmas
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Lack cell walss, sterols inplasma membrane.
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Archaea
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Wall-less or walls of pseudomurein (lack NAM and D- amino acids)
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Acid-Fast Cell Walls
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Like gram positive cell walls

waxy lipid (Mycolic acid)bound to peptidoglycan

Ex:Mycobacterium and Norcadia

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Damages to the Cell Wall
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  • Lysosyme: digests discharide in peptidoglycan
  • Penicilin inhibits peptide bridges in peptidoglycan
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Protoplast
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Is a wall-less cell,susceptible to osmotic lysis
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Spheroplast
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is a wall-less gram positive cell, suceptible to osmotic lysis
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L-Forms
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are wall-less cells that swell into regular shape
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The Plasma Membrane (Prokatyotes)

 

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consist primarily of Phospolipids,lipid bilayer,

Protein Molecules:

Peripheral proteins

integral proteins

Transmembrane proteins

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Fluid Mosaic Model
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arrangment of phospholipids and proteins that allow membrane proteins to move freely to perform their function w/o damaging the structure of the membrane
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Selective Permeability
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Allows or prevents passage of some molecules.
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Plasma Membrane Functions
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Contain enzymes that catalize chemical reactions that breakdown nutrients and produce ATP

Photosynthetic pigments on foldings called:chromatophores and thylakoids

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What happens if the Plasma Membrane is damaged
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damage by alcohols,detergents& polymyxin antibiotics cause leakage of cell contents
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Simple Diffusion
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movement of a substance(not water) accross membranes from an area of high concentration to an area of low concentration

Small & nonpolar molecule

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Facilitated Diffusion
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Facilitators of diffusion are channel or carrier

Transporters protein in the membrane assists molecule with transport accross the membrane

large and/or polar molecule H+

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Osmosis
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The movement of water accross a selectively permeable membrane from an area of high water to an area of lower water concentration
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Osmotic Pressure
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The pressure needed to stop the movement of water accross the membrane
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Movement of Water Accross Membrane Occurs Through:
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Lipid Layer

Aquaporins(water channels)

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Osmotic Solutions:
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Isotonic,Hypotonic and Hypertonic
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Osmic Solution Isotonic
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Equal solute concentration=H2O
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Osmic Solution Hypotonic
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Less solute concentration = more H2O
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Osmic Solution Hypertonic
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More solute concentration = less H2O
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Active Transpor
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requires a transporter protein and ATP
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Group Translocation
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Requires a transporter protein and PEP(phosphoenolpyruvic acid)
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Cytoplasma
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The substance inside the plasma membrane
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The Nucleoid (Prokaryotes)
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Bacterial chromosome attached to the plasma membrane
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Prokaryotic Ribosome
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Protein Synthesis

70s

50s + 30s subunits

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Inclusions are:
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Reserve Deposits
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7 Types of Inclusion in Prokaryotes
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  • Metachromatic granules(volutin)-Phosphate reserve
  • Polysaccharide granule-energy reserves
  • Lipid inclusions-energy reserves
  • Sulfur granules-energy reserves
  • Carboxysomes-Ribulose, 1.5 disphosphate carboxylase for CO2 fixation
  • Gas Vacuoles-Protein covered cylinders
  • Magnetosomes-Iron oxide (destroys hydrogen peroxide H2 O2)
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Endospores
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Resting Cells 

Resistant to desiccation,heat,chemicals

Ex: Bacillus, Clostridium

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Sporulation
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Endospore Formation
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Germination
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Endospore return to vegetative state
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