Microbiology Lab Practice Test – Flashcards

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Lobbyist
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Contracting a public official - to persuade the official to support the groups interest
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Semi-solid tryptone soy broth is used for growing bacteria in Petri dishes.
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false
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Pure colonies can develop from one single bacterial cell or a colony forming unit.
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true
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Broth tubes can be used to exhibit morphology features such as color, texture, andelevation.
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false
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An organism that can survive in a pH of 2 would be called a neutrophile.
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false
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Anaerobes can be found in the soil and can cause human infections.
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true
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A differential medium enables you to tell the difference between bacteria and viruses.
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false
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Fungal spores would be able to withstand 100C heat, just like bacterial endospores can.
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false
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Fungi that can cause human disease include the genera Trichphyton and Candida.
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true
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Nonhalophile bacteria would burst when exposed to 15% salt (NaCl) concentration.
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false
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All disinfectant chemicals are as potent against gram positive organisms as they areagainst gram negative organisms.
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false
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Which of the following techniques would not exhibit motility:
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negative stain
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A good selective medium used to grow molds would be:
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Sabourauds Agar
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Pellicles, turbidity, and flocculent formations of bacteria can be seen in:
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broth tubes
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The motility test medium is:
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an agar deep with TTC
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Thioglycollate added to a culture tube would:
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act to create anaerobic conditions
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Which of the following is true concerning agar (agar-agar):
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Is dissolved into media by warming and stirring.
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Gram positive bacilli:
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Include disease causing members such as tetanus and anthrax.
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Which of the following is not usually observed on agar plates:
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turbidity
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Flagella found all over the cell is an arrangement called:
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amphitrichous
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Molds are characterized by
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Type of asexual spore.
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A complex culture medium for bacteria, would be similar to hot dogs for humans (hot dogs are made out of a mix of meats, and rarely do we know exactly what’s in them).
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true
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A rodac plate is normally inoculated by means of sterile swabs.
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false
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The hanging drop procedure involves the use of a plain glass slide, a cover slip, and adrop of liquid.
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false
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Anaerobes can grow in all the following except:
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Candle jars
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When a methylene blue pad is placed in an anaerobe jar and it turns blue, this would mean:
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that the system did not work and air is still present.
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Brownian movement is seen as a purposeful darting motion which is caused by tiny bacterial pili.
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false
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Ubiquitous means:
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microbes
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A pure culture is the same as an isolated mixed culture.
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false
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A disinfectant that is bacteriocidal will only stop the growth of bacteria, and won’t kill it.
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false
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Which of the following is true of disinfectants?
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They are important to use when handling food, in order to avoid contamination with Salmonella, Clostridium, E. coli, or other food spoilers.
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Increased UV exposure to bacteria causes higher rates of cell death, because UV light causes:
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pyrimidine (thymine) dimers
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Gram positive cocci are commonly found on toilet seats and chairs becausecommon skin commensals.
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true
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All motile bacteria produce a red pigment that turns motility deeps red when they move.
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false
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Bacteria that can grow well from 0C up to 15C, but do not grow at higher temperatures, would probably be classified as
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psychrophiles
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Drastically increased temperature and drastically acidic or basic pH, for organisms considered meosphiles and neutrophiles, would have what effect?
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cause denaturation of proteins, enzymes, and membranes
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Which of the following is true of disinfectants?
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can be evaluated by immersing a disk in the disinfectant, placing it on a lawn of bacteria, and measuring the inhibiting of growth around the disk after incubation.
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List and describe two methods to grow anaerobes
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anaerobic chamber, anaerobe jar
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while anaerobes cannot survive in oxygen
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do not possess the defenses that make aerobic life possible and therefore cannot survive in air
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What makes aerobes able to survive in oxygen
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The excited singlet oxygen molecule is very reactive. Therefore, superoxide must be removed for the cells to survive in the presence of oxygen.
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