Microbiology Gram Pos. Rods – Flashcards
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| Corynebacterium diptheria |
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| Gram positive rods, L or V |
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| Corynebacterium diptheria |
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| potassium tellurite shows black colonies |
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| Corynebacterium diptheria |
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| Elek test shows precipitin line |
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| Corynebacterium diptheria |
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| Elek test shows precipitin line |
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| Elek test precipitin line from... |
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| immunocomplexs formed by antitoxin from test strip and toxin from bacteria = black color |
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| Corynebacterium diptheria |
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| grown on Loeffler's coagulated blood serum medium with methylene blue, polymerized metaphosphate granules appear beaded are babes ernst granules |
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| Corynebacterium diptheria |
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| Elek test positive |
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| Corynebacterium diptheria, Bacillus anthracis, Listeria monotcytogenes, Bacillus cereus |
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| aerobic |
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| diptheria toxin |
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| this A/B toxin ADP ribosylates EF-2 |
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| Corynebacterium diptheria |
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| pseudomembrane bleeds bc of inflammatory cells which contain fibrin |
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| HBEGF on heart cells and nerve cells |
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| diptheria toxin binds this factor |
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| Corynebacterium diptheria |
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| low Fe = high toxin |
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| Corynebacterium diptheria |
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| toxin encoded on lysogenic beta phage |
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| URTI, pharyngitis, bilateral cervical lymph enlargement, pseudomembrane |
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| sypmtoms of diptheria |
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| Corynebacterium diptheria, GAS, and others |
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| causes pharyngitis |
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| Corynebacterium diptheria |
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| transmitted by respiratory droplets |
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| fibrin, leukocytes, necrotic ep cells, bacteria cells, Corynebacterium diptheria |
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| pseudomembrane on pharynx is made of... and is involved with this bug |
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| Corynebacterium diptheria |
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| treat with antitoxin, penicillin/erythromycin, DPT vx |
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| Corynebacterium diptheria DTaP |
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| associated with herd immunity |
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| Clostridium dificile |
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| can cause peritoneal abscess |
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| Clostridium dificile, Clostridium botulinum |
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| gram + rod has some pink stain (spores) |
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| Clostridium dificile, Clostridium botulinum, Bacillus anthracis, Clostridium perfringens |
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| forms spores |
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| Clostridium dificile, Clostridum botulinum, Clostridium tetani, Clostridium perfringens |
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| anaerobic |
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| toxin B from Clostridium dificile |
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| this toxin causes CPE |
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| toxin A of Clostridium dificile |
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| this toxin causes fluid production and mucosal damage in GI, enterotoxin effects |
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| Clostridium dificile |
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| antibiotic associated pseudomembranous colitis |
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| Clostridium dificile and rotavirus |
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| causes watery diarrhea and vomiting |
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| Clostridum botulinum |
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| mouse protection tests |
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| Clostridum botulinum |
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| lysogenic conversion by phage |
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| Clostridum botulinum |
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| proteolytic enzymes form gas in body and in can |
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| Clostridum botulinum |
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| produces 8 toxins |
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| A B E |
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| most important toxins for Clostridum botulinum |
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| toxins from Clostridum botulinum |
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| toxin absorbed from gut, to bloodstream, to PNS, protease cleaves proteins involved in Ach release |
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| botulism |
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| has a wound, infant, and food version |
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| Clostridum botulinum |
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| diplopia, ascending paralysis, dilated pupils |
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| strep pn, Clostridum botulinum, and others |
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| causes food poisoning |
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| food botulism |
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| ds caused by preformed toxin |
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| Clostridum botulinum |
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| trivalent antitoxin made in horses |
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| botulin toxin |
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| heat labile toxin |
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| Bacillus anthracis, Listeria monotcytogenes |
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| can be found in CSF |
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| Bacillus anthracis |
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| gram + rods, boxcars in chains |
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| Bacillus anthracis |
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| antiphagocytic capsule made of D=glutamate |
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| Bacillus anthracis |
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| non hemolytic |
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| Bacillus anthracis |
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| pxo1 and px02 plasmids |
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| px01 |
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| plasmid encodes exotoxin PA, LF, EF |
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| EF of Bacillus anthracis |
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| causes inc cAMP fluid out of cells |
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| Bacillus anthracis |
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| PA BP important for the vx for... |
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| px02 |
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| plasmid encodes poly d glu capsule |
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| Bacillus anthracis |
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| gamma phage assay is a bacteriophage that specifically binds and lyses... |
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| inhalation anthrax |
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| enlarged mediastinum |
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| inhalation anthrax |
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| enlarged mediastinum |
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| Bacillus anthracis |
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| causes high neutrophils |
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| Bacillus anthracis |
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| causes inhalation and cutaneous dss |
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| Bacillus anthracis, hantavirus, flavivirus, togavirus |
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| cause fever, vomiting, elarged mediastinum? |
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| Bacillus anthracis |
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| no person to person infection in... |
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| Bacillus anthracis |
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| Tx with cipro or doxycycline |
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| supresses cytokine response |
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| what does LF of anthrax do? |
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| Clostridium tetani |
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| infects umbilicus |
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| Clostridium tetani |
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| forms a terminal spore at end of rod |
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| Clostridium tetani |
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| exotoxin has A and B domain |
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| Clostridium tetani |
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| local infection, systemic toxin spread, retrograde to CNS, GABA and glycine, neurons constantly firing |
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| Clostridium tetani |
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| no tests |
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| Clostridium tetani |
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| only 1 serotype |
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| Clostridium tetani |
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| plasmid encodes something important? |
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| spasm of face with tetanus neonatorum |
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| risus sardonicus |
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| Clostridium tetani, b. anracis |
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| spores in soil |
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| Clostridium tetani |
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| poor 02 supply makes u susceptible to... |
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| Clostridium tetani |
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| antitoxin is immune globulin |
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| Clostridium tetani |
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| tx with metronidazole, toxoid, pen G, resp support |
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| Listeria monotcytogenes |
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| small beta hemolytic colonies |
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| Listeria monotcytogenes |
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| motility umbrella agar |
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| Listeria monotcytogenes |
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| escapes macrophage |
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| phospholipase C and listeriolysin O |
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| L. mono escapes mac phagosome with... |
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| Listeria monotcytogenes |
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| actin rocket |
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| Listeria monotcytogenes |
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| heat-stable spores |
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| Listeria monotcytogenes |
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| pseudopodia |
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| Listeria monotcytogenes |
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| ActA receptor binds actin |
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| Listeria monotcytogenes,GBS, N meningitis, and many others |
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| meningitis? |
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| Listeria monotcytogenes |
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| can be confused for cocci |
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| Listeria monotcytogenes |
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| catalase positive |
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| Listeria monotcytogenes |
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| transmitted transplacentally |
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| Listeria monotcytogenes |
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| tx with ampicillin and trimethoprim-sulfamethoxazole |
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| Clostridium perfringens |
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| alpha toxin causes incomplete hemolysis beta toxin causes complete hemolysis == double hemolysis |
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| Clostridium perfringens |
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| litmus milk test shows organism produces H2o2 and CO |
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| lecithinase (phospholipase C) - breaks down phospholipids of eu membranes |
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| alpha toxin mode of action |
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| Clostridium perfringens |
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| theta toxin (hemolysis), DNAase, hyaluronidase, collagenase, hemolysin |
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| Clostridium perfringens |
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| Naeglar egg test shows precipitation in yolk agar |
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| Clostridium perfringens |
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| causes Gas gangrene |
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| Clostridium perfringens |
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| veg. cells are normal flora of colon and vagina |
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| Clostridium perfringens |
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| local infection with systemic toxemia grow in traumatized tissue, esp muscle |
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| Clostridium perfringens |
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| tx with hyperbaric O2 chamber to suppress bacteria and promote angiogenesis |
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| Bacillus cereus |
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| fried rice |
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| Bacillus cereus |
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| makes a toxin that inc cAMP and a superAg |
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| heat stabile - emetic and heat labile - diarrhea |
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| 2 forms of Bacillus cereus gastroenteritis |
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| Bacillus cereus, Staph aureus |
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| food poisoning |
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| bacillus, clostridium, cornybacterium, listeria |
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| 4 gram positive rods |
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| bacillus and clostridium |
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| form spores |
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| bacillus |
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| aerobic |
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| clostridium |
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| not aerobic |
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| bacillus and clostridium |
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| gram + rods long deeply staining |
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| cornybacterium |
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| club shaped G + rods |
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| cornybacterium and listeria |
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| V or L G+ rods |
