Microbiology 12 – Flashcards
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Large structures (e.g., ribosomes) form spontaneously from their macromolecular components by a process known as __________.
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self-assembly
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Eukaryotic cells compartmentalize some anabolic and catabolic pathways so that these pathways can be operate ______________.
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independently
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Cells save energy and materials by using many of the same enzymes for both __________ and __________.
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anabolism; catabolism
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Different proteins have different amino acid __________.
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sequences
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Many enzyme catalyzed reactions are freely reversible; however, some key steps require separate enzymes for the reverse step in order to provide for independent regulation of anabolic and catabolic pathways.
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TRUE
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Nongrowing cells continuously degrade and resynthesize cellular molecules during a process known as cellular turnover.
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TRUE
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The use of compartmentation for the simultaneous but independent regulation of anabolic and catabolic pathways occurs generally in eukaryotes but not in prokaryotes.
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TRUE
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Anabolic and catabolic pathways use different cofactors for similar reactions.
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TRUE
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In the synthesis of amino acids the addition of the amino nitrogen usually occurs __________ the synthesis of the carbon skeletons.
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after
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Although most autotrophic microorganisms fix carbon dioxide using the Calvin-Benson cycle, this pathway is absent in the Archaea.
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TRUE
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Photoautotrophs are the only organisms that can incorporate (fix) carbon dioxide into biomolecules.
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FALSE
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Because peptidoglycan lies outside the plasma membrane, all steps in the complex synthesis process of this molecule takes place outside the cell.
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FALSE
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Nitrogen fixation is used to form ATP, which is then used for energy reserves.
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FALSE
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In the biosynthesis of nucleotides, pyrimidines are assembled starting with ribose-5-phosphate while ribose-5-phosphate is added after synthesis of the nitrogenous base for purines.
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FALSE
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Unsaturated fatty acids are those containing carbon-carbon ________ bonds.
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double
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Bacteria can synthesize unsaturated fatty acids only under anaerobic conditions.
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FALSE
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Which of the following is true about the synthesis of macromolecules from monomeric subunits?
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A. It saves genetic storage capacity.
B. It saves biosynthetic raw materials.
C. It saves energy.
D. all of the choices
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Synthesis of complex molecules from simpler ones with the input of energy is called
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anabolism.
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There are __________ common amino acids used to synthesize proteins.
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2021
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Which of the following is a source of carbon skeletons that are used in the synthesis of amino acids?
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A. Acetyl-CoA.
B. TCA cycle intermediates.
C. Glycolysis intermediates.
D. All of the choices.
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Assemblies of macromolecules are referred to as __________ complexes.
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supramolecular
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Microorganisms assimilate large amounts of all of the following except __________ into organic molecules.
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sodium
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The process of carbon dioxide fixation refers to
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reduction of carbon dioxide and incorporation into organic molecules that are assimilated.
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Which pathway(s) are used by eukaryotic microorganisms to fix carbon dioxide?
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Calvin cycle.
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Which pathway(s) is used to fix carbon dioxide?
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Reductive tricarboxylic pathway.
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Which of the following is not a phase of the Calvin Cycle?
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oxidation phase
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In order to form a single molecule of glucose, it takes __________ turns through the reactions of the Calvin cycle
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2
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Large numbers of small monomeric molecules join together to form large molecules that are known as:
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macromolecules
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Photoautotrophs use light energy directly for
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the production of ATP and NADPH.
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The photosynthetic production of one molecule of glucose requires __________ molecule(s) of NADPH.
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12
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The photosynthetic production of one molecule of glucose requires __________ molecule(s) of ATP.
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18
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The series of enzymatic steps that fixes carbon dioxide into carbohydrate is called the ______.
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Calvin cycle
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The synthesis of glucose from non-carbohydrate precursors is called
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gluconeogenesis.
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In order to synthesize new peptidoglycan for bacterial cell walls, the existing walls must be partially digested by __________ to provide acceptor ends for the new synthesis.
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autolysins
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__________ is the carrier molecule required to transport peptidoglycan subunits to the location where they will be incorporated into the growing bacterial cell wall.
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Bactoprenol
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__________ reactions are used to replace TCA cycle intermediates that have been used to provide carbon skeletons for amino acid biosynthesis.
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Anaplerotic
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Symbiotic nitrogen fixation can consume up to __________% of the ATP generated by the host plant.
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20
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The major reductive amination pathway for incorporation of nitrogen into amino acids initially involves the production of __________ from alpha-ketoglutarate.
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glutamate
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Most microorganisms assimilate nitrogen as
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nitrate
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The reduction of sulfate as a terminal electron acceptor during anaerobic respiration is called __________ sulfate reduction.
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dissimilatory
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The reduction of sulfate for use in the production of compounds such as cysteine is called __________ sulfate reduction.
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assimilatory
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Many microbes use pyruvate carboxylase or phosphoenolpyruvate carboxylase to
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synthesize oxaloacetate.
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The reduction of atmospheric nitrogen to ammonia is called
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nitrogen fixation
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Nitrogen fixation depends on the enzyme nitrogenase which is produced by some
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bacteria archaea but no eukaryotes
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Phosphorous is usually assimilated by microorganisms as
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phosphate
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Which of the following cofactors is very important in the synthesis of purines?
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folic acid
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Assimilation of organic phosphates from the surroundings uses enzymes known as
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phosphatases.
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Purines and pyrimidines are precursors for synthesis of
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nucleic acids.
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A purine or pyrimidine that is attached to a sugar, either ribose or deoxyribose, to which is attached one or more phosphate groups is called a:
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nucleotide
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A purine or pyrimidine that is attached to a sugar, either ribose or deoxyribose, is called a
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nucleoside
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__________ are cyclic nitrogenous bases with two rings and pronounced aromatic properties.
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Purines
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Which of the following is not generally true about fatty acids produced by microorganisms?
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Gram-positive bacteria often make cyclopropane fatty acids.
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Which of the following do(es) not contribute to the construction of membrane phospholipids?
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Nucleotide biosynthesis.
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Unsaturated fatty acids are those containing __________ carbon-carbon double bond(s).
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1 or more
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Enzymes catalyze a reaction by
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decreasing the activation energy of the reaction.
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In the TCA cycle, two carbons in the form of __________ are added to oxaloacetate at the start of the cycle.
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acetyl-CoA 21.