Inhibition of Alcohol Dehydrogenase Essay Example

and in the presence of 1. 0 :M inhibitor. The data are presented in Table 13.

1 . Table 13. 1: Inhibition of mouse ADHI by N,1-5-dimethylhexylformamide Ethanol Concentration, mM ADHI velocity, ) NADH absorbance/min (without inhibitor) absorbance/min (with nhibitor) 120 1036 1022 25 1042 1024 1027 1065 1029 1033 a. What are the KM and Vmax values for ADH in the absence of inhibitor? in the presence of the inhibitor? b. What type of inhibitor is N-l ,5-dimethylhexylformamide?

Explain.

c. Calculate the 'alues of " and/or v", if they are significantly different from 1 . What kind of inhibitor is N-l ,5-dimethylhexylformamide? Explain. d. Calculate the Ki and/or Ki' (whichever is appropriate) for N-l dimethylhexylformamide (Hint: You can obtain these values from " and 4.

The authors describe the mechanism of ADH as an "ordered bi-bi" mechanism. Give a written description of the mechanism, as shown in Figure 13. . How does N-l ,5dimethylhexylformamide inhibit the activity of the ADH enzyme?

How does N-l ,5dimethylhexylformamide differ from the "classic" inhibitors of this type that are described in our textbook? 5. The authors found that a class of compounds called pyrazoles were also inhibitors of ADH.

These inhibitors bind to the E-NAD+ complex. What kind of inhibitor are pyrazoles? Are these inhibitors the same or different as the formamides? 5. a. Would N-l ,5-dimethylhexylformamide be an effective alternative for the treatment of methanol and ethylene glycol poisoning, assuming that it is non-toxic self (and as an alternative to getting the patient drunk, as described.